The presence of Chlamydia phage PhiCPG1 capsid protein VP1 genes and antibodies in patients infected with Chlamydia trachomatis

• Authors: Jingyue Ma , Yuan Liu , Yuanjun Liu , Lingjie Li , Shuping Hou , Xibo Gao , Manli Qi , Quanzhong Liu
• Languages of publication: EN

Abstracts

EN

Chlamydia phage PhiCPG1 has been found in Chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that Chlamydia phage is also present in patients infected with C. trachomatis (Ct). In the present study, we assayed for presence of Chlamydia phage capsid protein VP1 genes and antibodies in 84 non-Ct controls and 206 Ct patients using an enzyme-linked immunoassay (ELISA), followed by verification with Western blot. None of the subjects were exposed to an antibiotic treatment or had a C. pneumoniae infection. The VP1 antibody test was positive in both, the ELISA and Western blot assay, in 4 Ct patients. PCR amplification experiments revealed presence of the VP1 gene in 5 Ct patients. The results suggest that Chlamydia phage capsid protein VP1 may exist in some Ct patients.

Keywords

EN

Chlamydia trachomatis; Chlamydia bacteriophage; capsid protein; VP1

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 3
• Pages: 501-504

Dates

published

2016

received

2015-09-07

revised

2015-12-30

accepted

2016-03-11

(unknown)

2016-05-20

Contributors

author

Jingyue Ma

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Yuan Liu

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Yuanjun Liu

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Lingjie Li

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Shuping Hou

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Xibo Gao

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Manli Qi

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

author

Quanzhong Liu

• Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China

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Identifiers

DOI

10.18388/abp.2015_1185