Bacterial endophytes of Trans-Ili Alatau region's plants as promising components of a microbial preparation for agricultural use

• Authors: Togzhan Mukasheva , Ramza Berzhanova , Lyudmila Ignatova , Anel Omirbekova , Yelena Brazhnikova , Raikhan Sydykbekova , Maya Shigaeva
• Languages of publication: EN

Abstracts

EN

In this study, 382 isolates of bacterial endophytes from tissues of plants grown in the foothills and piedmont plains of the Trans-Ili Alatau were isolated. It was found that certain strains actively produce indole-3-acetic acid (IAA) in a medium containing l-tryptophan. Among the strains studied, 26 strains (66%) showed a positive response to production of IAA. Bacteria synthesized IAA in the range of 18.6 ± 1.1 to 82.4 ± 2.3 µg/ml. IAA was synthesized more actively by Streptosporangium sp. KK1 (44.1 µg/ml), Rhodococcus sp. KK 2 (42.5 µg/ml), Streptomyces tendae KK3 (44.9 µg/ml) strains. The most active auxin's producer is a Jeotgalicoccus halotolerans BAK1 strain, whose total level of IAA production reached 82.4 µg/ml. Such strains as Streptomyces griseorubiginosis KK4, Streptomyces sp. KK5 and Jeotgalicoccus halotolerans BAK1 were found to have a significant stimulating effect which was reflected in the increase of the length of the roots of soybean and barley. As a result, 8 promising strains with fungicidal, growth-stimulating, phosphorus dissolving and enzymatic activities were selected for the further development of highly microbiological preparations for crop research.

Keywords

EN

bacterial endophytes; growth-stimulating effect; plant growth-promoting bacteria; 3-indolylacetic acid; barley; soybean

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 2
• Pages: 321-328

Dates

published

2016

received

2015-07-31

revised

2015-12-18

accepted

2016-01-24

(unknown)

2016-03-31

Contributors

author

Togzhan Mukasheva

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

author

Ramza Berzhanova

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

author

Lyudmila Ignatova

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

author

Anel Omirbekova

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

author

Yelena Brazhnikova

• Research Institute of Biology and Biotechnology Problems, Almaty, Kazakhstan

author

Raikhan Sydykbekova

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

author

Maya Shigaeva

• Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan

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Identifiers

DOI

10.18388/abp.2015_1157