Probiotic preparation reduces faecal water genotoxicity and cytotoxicity in chickens fed ochratoxin A contaminated feed (in vivo study)

• Authors: Katarzyna Śliżewska , Adriana Nowak , Stefania Smulikowska
• Languages of publication: EN

Abstracts

EN

The aim of the present study was to assess the genotoxicity and cytotoxicity of the faecal water of chickens fed ochratoxin A (OTA) contaminated feed with and without probiotic preparation. The study was performed on 20 healthy female Ross broiler chickens divided into 4 groups: control chickens - fed with non-supplemented feed; PP chickens - fed feed supplemented with the probiotic preparation; OTA chickens - fed feed contaminated with 1 mg per kg of OTA; OTA + PP chickens - fed feed contaminated with 1 mg per kg of OTA and supplemented with the probiotic preparation. Faecal water samples were collected on the 35th day of life of chickens from each group. Genotoxicity was measured using the comet assay, and cytotoxicity by means of MTT tests. Mean DNA damage, measured as the percentage of DNA in the tails of the comets, was 8.50 ± 1.10 for chickens fed OTA at 1 mg/kg and 6.41 ± 0.67 in the controls. The supplementation of feed with the probiotic preparation decreased the extent of DNA damage to 4.74 ± 0.78. In the control group of chickens the average cytotoxicity was 38.5 ± 0.5 (in MTT), while in the probiotic preparation group (PP group) it was 31.8 ± 0.7 (in MTT). After supplementation of the feed with the probiotic preparation, the genotoxicity and cytotoxicity were decreased in a statistically significant manner.

Keywords

EN

ochratoxin A; probiotics; chicken; genotoxicity; cytotoxicity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 2
• Pages: 281-286

Dates

published

2016

received

2015-07-15

revised

2015-09-08

accepted

2015-10-09

(unknown)

2016-01-07

Contributors

author

Katarzyna Śliżewska

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland

author

Adriana Nowak

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland

author

Stefania Smulikowska

• Polish Academy of Sciences, The Kielanowski Institute of Animal Psysiology and Nutrition, Jabłonna, Poland

References

• Akande KE, Abubaker MM, Adegbola TA, Bogoro SE (2006) Nutritinal and health implications of mycotoxins in animal feeds: a review. Pakistan J Nutr 5: 398-403.
• Allameh A, Safamehr A, Mirhandi S, Shivazad M, Razzaghi-Abyaneh M, Afshar-Naderi A (2005) Evaluation of biochemical and production parameters of broiler chicks fed ammonia treated aflatoxin contaminated maize grains. Anim Feed Sci Tech 122: 289-301. doi: 10.1016/j.anifeedsci.2005.03.005.
• Bayman P, Baker JL (2006) Ochratoxins: A global perspective. Mycopathologia 162: 215-223. doi: 10.1007/s11046-006-0055-4.
• Blasiak J, Kowalik J (2000) A comparison of the in vitro genotoxicity of tri- and hexavalent chromium. Mutat Res 469: 135-145. doi: 10.1016/S1383-5718(00)00065-6.
• Castegnaro M, Mohr U, Pfohl-Leszkowicz A, Estève J, Steinmann J, Tillman T, Michelon J, Bartsch H (1998) Sex- and strain-specific induction of renal tumors by ochratoxin A in rats correlates with DNA adduction. Int J Cancer 77: 70-75. doi: 10.1002/(SICI)1097-0215(19980703).
• Creppy EE, Chiarappa P, Baudrimont I, Borracci P, Moukha S, Carratù MR (2004) Synergistic effects of fumonisin B1 and ochratoxin A: are in vitro cytotoxicity data predictive of in vivo acute toxicity? Toxicology 201: 115-123. doi: 10.1016/j.tox.2004.04.008.
• de Groene EM, Hassing IG, Blom MJ, Seinen W, Fink-Gremmels J, Horbach GJ (1996) Development of human cytochrome P450-expressing cell lines: application in mutagenicity testing of ochratoxin A. Cancer Res 56: 299-305.
• Dietrich DR, O'Brien E, Stack ME, Heussner AH (2001) Species- and sex-specific renal cytotoxicity of ochratoxin A and B in vitro. Exp Toxicol Pathol 53: 215-225.
• Doorten YS, Nijmeijer S, de Nijs-Tjon L, Fink-Gremmels J (2006) Metabolism-mediated Ochratoxin A genotoxicity in the single-cell gel electrophoresis (Comet) assay. Food Chem Toxicol 44: 261-270. doi: 10.1016/j.fct.2005.07.009.
• El Adlouni C, Pinelli E, Azémar B, Zaoui D, Beaune P, Pfohl-Leszkowicz A (2000) Phenobarbital increases DNA adduct and metabolites formed by ochratoxin A: role of CYP 2C9 and microsomal glutathione-S-transferase. Environ Mol Mutagen 35: 123-131. doi: 10.1002/(SICI)1098-2280(2000).
• El-Nezami H, Mykkänen H, Kankaanpää P, Salminen S, Ahokas J (2000) Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodeum. J Food Prot 63: 549-552.
• FAO/WHO (2001) Safety evaluation of certain mycotoxins in food. Joint FAO/WHO Expert Committee on Food Additives, 56th Meeting, Geneva, 6-15 February.
• Faucet V, Pfohl-Leszkowicz A, Dai J, Castegnaro M, Manderville RA (2004) Evidence for covalent DNA adduction by ochratoxin A following chronic exposure to rat and subacute exposure to pig. Chem Res Toxicol 17: 1289-1296. doi: 10.1021/tx049877s.
• Fraga ME, Curvello F, Gatti MJ, Cavaglieri LR, Dalcero AM, da Rocha Rosa CA (2007) Potential aflatoxin and ochratoxin A production by Aspergillus species in poultry feed processing. Vet Res Commun 31: 343-353. doi: 10.1007/s11259-006-3434-x.
• Fuchs S, Sontag G, Stidl R, Ehrlich V, Kundi M, Knasmüller S (2008) Detoxification of patulin and ochratoxin A, two abundant mycotoxins, by lactic acid bacteria. Food Chem Toxicol 46: 1398-1407. doi: 10.1016/j.fct.2007.10.008.
• Goldblatt LA (1969) Aflatoxin. Academic Press, New York, USA.
• Haskard CA, El-Nezami H, Kankaanpää P, Salminen S, Ahokas J (2001) Surface binding of aflatoxin B1 by lactic acid bacteria. Appl Environ Microbiol 67: 3086-3091. doi: 10.1128/AEM.67.7.3086-3091.2001.
• Heussner AH, Dietrich DR, Brien EO (2006) In vitro investigation of individual and combined cytotoxic effects of ochratoxin A and other selected mycotoxins on renal cells. Toxicol in Vitro 20: 332-341. doi: 10.1016/j.tiv.2005.08.003.
• Höhler D (1998) Ochratoxin A in food and feed: occurrence, legislation and mode of action. Z Ernahrungswiss 37: 2-8.
• IARC (1993) Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. In IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans. vol. 59, Lyon, France.
• Knasmüller S, Steinkellner H, Hirschl AM, Rabot S, Nobis EC, Kassie F (2001) Impact of bacteria in dairy products and of the intestinal microflora on the genotoxic and carcinogenic effects of heterocyclic aromatic amines. Mutat Res 480-481: 129-138. doi: 10.1016/S0027-5107(01)00176-2.
• Lutz W, Gaylor D (1996) Significance of DNA adducts at low dose: shortening the time to spontaneous tumor occurrence. Regul Toxicol Pharmacol 23: 29-34. doi: 10.1006/rtph.1996.0005.
• Madrigal-Santillán E, Madrigal-Bujaidar E, Márquez-Márquez R (2006) Antigenotoxic effect of Saccharomyces cerevisiae on the damage produced in mice fed with aflatoxin B1 contaminated corn. Food Chem Toxicol 44: 2058-2063. doi: 10.1016/j.fct.2006.07.006.
• Malaveille C, Brun G, Bartsch H (1991) Genotoxicity of ochratoxin A and structurally related compounds in Escherichia coli strains: studies on their mode of action. In Mycotoxins. Castegnaro M, Plestina R, Dirheimer G, Chernozemsky IN, Bartsch H, eds, pp 261-266. IARC Scientific Publications, Lyon.
• Michalowski P, Libudzisz Z, Śliżewska K, Klewicka E, Motyl I, Biernasiak J, Zabierski R (2012) Probiotic growth promoter for poultry. Licence number 212061.
• Miller EC (1981) Some current perspectives on chemical carcinogenesis in humans and experimental animals. Cancer Res 38: 1479-1496.
• Muscarella M, Palermo C, Rotunno T, Quaranta V, D'Antini P (2004) Survey of Ochratoxin A in Cereals from Puglia and Basilicata. Vet Res Commun 28: 229-232.
• Obrecht-Pflumio S, Dirheimer G (2000) In vitro DNA and dGMP adducts formation caused by ochratoxin A. Chem Biol Interact 127: 29-44. doi: 10.1016/S0009-2797(00)00169-1.
• Obrecht-Pflumio S, Grosse Y, Pfohl-Leszkowicz A, Dirheimer G (1996) Protection by indomethacin and aspirin against genotoxicity of ochratoxin A, particularly in the urinary bladder and kidney. Arch Toxicol 70: 244-248.
• Pinelli E, Adlouni C, Pipy B, Quartulli F, Pfohl-Leszkowicz A (1999) Roles of cyclooxygenase and lipoxygenases in ochratoxin A genotoxicity in human epithelial lung cells. Environ Toxicol Pharmacol 7: 95-107. doi: 10.1016/S1382-6689(99)00008-3.
• Puntaric D, Bosnir J, Smit Z, Skes I, Baklaic Z (2001) Ochratoxin A in corn and wheat: geographical association with endemic nephropathy. Croat Med J 42: 175-180.
• Rocha MEB, Freire FChO, Maia FEF, Guedes MIF, Rondina D (2014) Mycotoxins and their effects on human and animal health. Food Control 36: 159-165. doi: 10.1016/j.foodcont.2013.08.021.
• Sangare L, Zhao Y, Folly YM, Chang J, Li J, Selvaraj JN, Xing F, Zhou L, Wang Y, Liu Y (2014) Aflatoxin B1 degradation by a Pseudomonas strain. Toxins 6: 3028-3040. doi: 10.3390/toxins6103028.
• Schaaf GJ, Nijmeijer SM, Maas RF, Roestenberg P, de Groene EM, Fink-Gremmels J (2002) The role of oxidative stress in the ochratoxin A-mediated toxicity in proximal tubular cells. Biochem Biophys Acta 1588: 149-158. doi: 10.1016/S0925-4439(02)00159-X.
• Shetty P, Jespersen L (2006) Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends Food Sci Tech 17: 48-55. doi: 10.1016/j.tifs.2005.10.004.
• Siegel D, Babuscio T (2011) Mycotoxin management in European cereal trading sector. Food Control 22: 1145-1153. doi: 10.1016/j.foodcont.2011.02.022.
• Singh N, McCoy M, Tice R, Schneide E. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191. doi: 10.1016/0014-4827(88)90265-0.
• Śliżewska K, Libudzisz Z, Piotrowska M, Smulikowska S, Czerwinski J (2012) The new use of probiotic preparation. Licence Number 210685.
• Slizewska K, Nowak A, Libudzisz Z, Blasiak J (2010) Probiotic preparation reduces the faecal water genotoxicity in chickens fed with aflatoxin B1 contaminated fodder. Res Vet Sci 89: 391-395. doi: 10.1016/j.rvsc.2010.04.004.
• Stidl R, Fuchs S, Koller V, Marian B, Sontag G, Ehrlich V, Knasmüeller S (2007) DNA-protective properties of lactic acid bacteria. In Synthetic and Natural Compounds in Cancer Therapy and Prevention. Durackova Z, Slamenova D, eds. Bratislava, Slovakia.
• Ŝtyriak I, Čonková E, Kmec V, Böhm J, Razzazi E (2001) The use of yeast for microbial degradation of some selected mycotoxin. Mycotoxin Res 17: 24-27.
• Topcu A, Bulat T, Wishah R, Boyaci IH (2010) Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains. Int J Food Microbiol 139: 202-205. doi: 10.1016/j.ijfoodmicro.2010.03.006.
• Van der Merwe KJ, Steyn PS, Fourie L, Scott DB, Theron JJ (1965) Ochratoxin A, a Toxic Metabolite produced by Aspergillus ochraceus Wilh. Nature 205: 1112-1113. doi: 10.1038/2051112a0.
• Vinderola G, Ritieni A (2015) Role of probiotics against mycotoxins and their deleterious effects. J Food Res 4: 10-21. doi: 10.5539/jfr.v4n1p10.

Identifiers

DOI

10.18388/abp.2015_1094