Preferences help
enabled [disable] Abstract
Number of results
2016 | 63 | 2 | 267-271
Article title

The relation between glutathione S-Transferase M1 null-genotype and cardiac problems in beta-thalassemia

Title variants
Languages of publication
This work was carried out to investigate the role of Glutathione S-Transferase M1 (GSTM1) null genotype frequency in prognosis of β-thalassemia, and to detect the correlation between GSTM1 null genotype and appearance of cardiac complications in β-thalassemia. Materials and Methods. The studied groups in the present work were divided to three groups (group I: 20 healthy subjects, group II: 56 β-thalassemic patients and group III: 16 β-thalassemic patients with cardiac complications were taken from group II). The measurement of human high sensitive C-reactive protein (hs-CRP) was performed using nephelometry. GSTM1 genotype was detected by Polymerase Chain Reaction (PCR) and cardiac complications were determined by using Echocardiography. Results. A statistically significant increase in hs-CRP and interleukin-6 (IL-6) levels was found in β-thalassemic patients with cardiac complications compared to normal subjects. Results showed no relation between GSTM1 null genotype frequency neither with β-thalassemia nor with cardiac complications appearance, where the interaction between GSTM1 null genotype in β-thalassemic patients with cardiac complications and healthy subjects were insignificant compared to subjects with GSTM1 non-null genotype. Conclusions. GSTM1 null genotype frequency has no role in β-thalassemia or cardiac complications appearance.
Physical description
  • Department of Immunogenetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt
  • Department of Chemistry, Faculty of Science, Cairo University, Cairo, Egypt
  • Department of Immunogenetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt
  • Department of Clinical Genetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt
  • Department of Immunogenetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt
  • Abdalla MY, Fawzi M, Al-Maloul SR, El-Banna N, Tayyem RF, Ahmad IM (2011) Increased oxidative stress and iron overload in Jordanian β-thalassemic children. Hemoglobin 35: 67-79. doi: 10.3109/03630269.2010.544624.
  • Adinarayana KPS, Kakara RR (2013) Estimation of homozygote recessive and heterozygous CDK3 distribution in randomly selected cancer subjects. J Evol Med Dental Sci 45: 8818-8822.
  • Aggeli C, Antoniades C, Cosma C, Chrysohoou C, Tousoulis D, Ladis V (2005) Endothelial dysfunction and inflammatory process in transfusiondependent patients with beta-thalassemia major. Int J Cardiol 105: 80-84.
  • Cantisani M, Vitiello M, Falanga A, Finamore E, Galdiero M, Galdiero S (2012) Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity. Int J Nanomedicine 7: 2361-2371. doi: 10.2147/IJN.S30467.
  • Chakarov I, Vlaykova T, Slavov E, Marinov R, Chakarova P (2014) Role of serum pro-hepcidin and GSTM1 and GSTT1 null polymorphisms for estimation of the risk of myocardial siderosis in children and 'young adults' with β-thalassemia major. Compe Clin Pathol 23: 725-733. doi: 10.1007/s00580-013-1677-9.
  • Clarke G, Higgins T (2000) Laboratory Investigation of Hemoglobinopathies and Thalassemias: review and update. Clin Chem 46: 1284-1290.
  • Erlandsen EJ, Randers E (2000) Reference interval for serum C-reactive protein in healthy blood donors using the Dade Behringm N Latex CRP mono assay. Scand J Clin Lab Invest 60: 37-43. doi: 10.1080/00365510050185029.
  • Galanello R, Origa R (2009) Management of thalassaemia. Iron 11: 264-285.
  • Hayes JD, Pulford DJ (1995) The glutathione S-Transferase supergene family: regulation of GST and the contribution of the lsoenzymes to cancer chemoprotection and drug resistance part II. Crit Rev Biochem Mol Biol 30: 521-600.
  • Jha R, Jha S (2014) Beta thalassemia-a review. J Pathol Nepal 4: 663-671. doi: doi: 10.3126/jpn.v4i8.11609.
  • Kremastinos DT, Farmakis D, Aessopos A, Hahalis G, Hamodraka E, Tsiapras D, Keren A (2010) β-Thalassemia cardiomyopathy history, present considerations, and future perspectives. Circ Heart Fail 3: 451-458. doi: 10.1161/CIRCHEARTFAILURE.109.913863.
  • Lamchiagdhase P, Pattanapanyasat K, Muangsup W (2000) Reticulocyte counting in thalassemia using different automated technologies. Laboratory Hematology 6: 73-78.
  • Matin S, Jahromi MG, Karemizadeh Z, Haghpanah S, De Sanctis V, Soliman A, Dehbozorgian J, Majd Z, Rezaei N, Karimi M (2015) The frequency of adrenal insufficiency in adolescents and young adults with thalassemia major versus thalassemia intermedia in Iran. Mediterr J Hematol Infect Dis 7: e2015005. doi: 10.4084/MJHID.2015.005.
  • Origa R, Satta S, Matta G, Galanello R (2008) Glutathione S-transferase gene polymorphism and cardiac iron overload in thalassaemia major. Br J Haematol 142: 143-145. doi: 10.1111/j.1365-2141.2008.07175.x.
  • Rabab MA, Bothina MH (2013) GSTM1 and GSTT1 polymorphism in Egyptian sickle cell anemia patients. Int J Hematol Oncol/UHOD: Uluslararasi Hematoloji Onkoloji Dergisi 23: 269-275.
  • Rajaram V, Evans AT, Caldito GC, Kelly RF, Fogelfeld L, Black HR, Doukky R (2011) High sensitivity C - reactive protein is associated with diastolic dysfunction in young African Americans without clinically evident cardiac disease. Open Cardiovasc Med J 5: 188-195. doi: 10.2174/1874192401105010188.
  • Sclafani S, Calvaruso G, Agrigento V, Maggio A, Nigro VL, D'Alcamo E (2013) Glutathione S transferase polymorphisms influence on iron overload in β-thalassemia patients. Thalassemia Reports 3, e6: 20-22. doi: 10.1007/s10528-015-9687-8.
  • Shi B, Ni Z, Cai H, Zhang M, Mou S, Wang Q, Qian J (2010) High-sensitivity C-reactive protein: an independent risk factor for left ventricular hypertrophy in patients with lupus nephritis. J Biomed Biotechnol 2010: 373426. doi: 10.1155/2010/373426.
  • Silverman RH, Klein EA, Weight CJ, Nguyen CT, Gupta JD (2009) Method for detection of xmrv. U.S. Patent Application 12/645, 181: 1-15.
  • Volpato S, Guralnik JM, Ferrucci L, Balfour J, Chaves P, Fried LP, Harris TB (2001) Cardiovascular disease, interleukin-6, and risk of mortality in older women. The Women's Health and Aging Study. Circulation 103: 947-953. doi: 10.1161/01.CIR.103.7.947.
  • Wilson MH, Grant PJ, Hardie LJ, Wild CP (2000) Glutathione S-transferase M1 null genotype is associated with a decreased risk of myocardialinfarction. FASEB J 14: 791-796.
  • Wu KH, Chang JG, Ho YJ, Wu SF, Peng CT (2006) Glutathione S-transferase M1 gene polymorphisms are associated with cardiac iron deposition in patients with beta thalassemia major. Hemoglobin 30: 251-256. PMID: 16798650.
  • Yousafzai YM, Khan S, Raziq F (2010) Beta-thalassaemia trait: Haematological parameters. J Ayub Med Coll Abbottabad 22: 84-86. PMID: 22455269.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.