Conflicting results of non-invasive methods for detection of Helicobacter pylori infection in children with celiac disease - a preliminary study

• Authors: Jan Józefczuk , Edyta Mądry , Jan Nowak , Marek Walkowiak , Klaudia Łochocka , Tomasz Banasiewicz , Andrzej Pławski , Jarosław Kwiecień , Jarosław Walkowiak
• Languages of publication: EN

Abstracts

EN

Background: There are no data addressing the usefulness of non-invasive tests for the detection of Helicobacter pylori (HP) infection in celiac disease (CD). Aim: The aim of this study was to compare two most sensitive and specific tests - urea breath test (UBT) and fecal antigen test (FAT) in HP diagnosis in CD patients. Materials and Methods: The study comprised of 76 CD patients, 49 healthy subjects (HS) and 35 patients who underwent differential diagnosis due to abdominal pain (AP patients). The presence of HP infection was evaluated using the 13C isotope-labeled UBT and FAT (ELISA). Results: HP infection was diagnosed based on UBT and FAT in 8 (16.3%) and 7 (14.3%) HS, and in 8 (10.5%) CD patients and 12 (34.3%) AP patients, respectively, using both tests. The prevalence of conflicting results in comparison with positive results (obtained with any of the two tests) was distinctly higher (54.5%) in CD group than in other subjects (23.3%); however, due to low HP prevalence, it did not reach the level of significance (p<0.1759). Conclusion: CD may increase the risk of divergent results of non-invasive tests used for the detection of HP infection in children. Since UBT is the most reliable test, we suggest its standard use as a method of choice in pediatric CD - at least until new evidence emerges supporting a different approach.

Keywords

EN

Helicobacter pylori; celiac disease; fecal test; breath test; urea

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 1
• Pages: 127-130

Dates

published

2016

received

2015-06-22

revised

2015-09-30

accepted

2015-12-21

(unknown)

2016-01-27

Contributors

author

Jan Józefczuk

• Pediatric Department with Children's Cardiologic Subunit, Specialist Hospital of Holy Spirit in Sandomierz, Sandomierz, Poland

author

Edyta Mądry

• Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland

author

Jan Nowak

• Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poznań, Poland

author

Marek Walkowiak

• Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poznań, Poland

author

Klaudia Łochocka

• Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poznań, Poland

author

Tomasz Banasiewicz

• Department of General, Gastroenterological and Endocrinological Surgery, Poznan University of Medical Sciences, Poznań, Poland

author

Andrzej Pławski

• Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland

author

Jarosław Kwiecień

• Department of Pediatrics, The School of Medicine and Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland

author

Jarosław Walkowiak

• Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poznań, Poland

References

• Kusters JG, Vliet AH, Kuipers EJ (2006) Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19: 449-490.
• Ekstrom AM, Held M, Hansson LE, Engstrand L, Nyren O (2001) Helicobacter pylori in gastric cancer established by CagA immunoblot as a marker of past infection. Gastroenterology 121: 784-791.
• WHO International Agency for Research on Cancer web side. Monographs on the evaluation of carcinogenic risks to humans. 1994; 61: schistosomes, liver flukes and Helicobacter pylori. http://monographs.iarc.fr/ENG/Monographs/. Access March 16th 2014.
• Drzymała-Czyż S, Kwiecień J, Pogorzelski A, Rachel M, Banasiewicz T, Pławski A, Szczawińska-Popłonyk A, Herzig KH, Walkowiak J (2013) Prevalence of Helicobacter pylori infection in patients with cystic fibrosis. J Cyst Fibros 12: 761-765.
• Jozefczuk J, Bancerz B, Walkowiak M, Glapa A, Nowak J, Piescikowska J, Kwiecień J, Walkowiak J (2015) Prevalence of Helicobacter pylori infection in pediatric celiac disease. Eur Rev Med Pharmacol Sci 19: 2031-2035.
• Żabka A, Kwiecień J, Bąk-Drabik K, Krzywicka A, Porębska J, Pieniążek W (2010) Does frequency of H. pylori in children 3-18 years old decrease? (Preliminary report). Probl Med Rodz 2: 18-20 (in Polish?).
• Husby S, Koletzko S, Korponay-Szabó IR, Mearin ML, Phillips A, Shamir R, Troncone R, Giersiepen K, Branski D, Catassi C, Lelgeman M, Mäki M, Ribes-Koninckx C, Ventura A, Zimmer KP; ESPGHAN Working Group on Coeliac Disease Diagnosis; ESPGHAN Gastroenterology Committee (2012) European Society for Pediatric Gastroenterology, Hepatology, and Nutrition guidelines for the diagnosis of coeliac disease. J Pediatr Gastroenterol Nutr 54: 136-160.
• Dzierzanowska-Fangrat K, Lehours P, Mégraud F, Dzierzanowska D (2006) Diagnosis of Helicobacter pylori infection. Helicobacter 11: 6-13.
• Asaka M, Kato M, Takahashi S, Fukuda Y, Sugiyama T, Ota H, Uemura N, Murakami K, Satoh K, Sugano K (2010) Guidelines for the management of Helicobacter pylori infection in Japan: 2009 revised edition. Helicobacter 15: 1-20.
• Malfertheiner P1, Megraud F, O'Morain CA, Atherton J, Axon AT, Bazzoli F, Gensini GF, Gisbert JP, Graham DY, Rokkas T, El-Omar EM, Kuipers EJ; European Helicobacter Study Group (2012) Management of Helicobacter pylori infection-the Maastricht IV/Florence Consensus Report. Gut 61: 646-664.
• Masoero G, Lombardo L, Della Monica P, Vicari S, Crocilla C, Duglio A, Pera A (2000) Discrepancy between Helicobacter pylori stool antigen assay and urea breath test in the detection of Helicobacter pylori infection. Dig Liver Dis 32: 285-290.
• Andersen LP, Rasmussen L (2009) Helicobacter pylori-coccoid forms and biofilm formation. FEMS Immunol Med Microbiol 56: 112-115.
• Casasola-Rodriguez B, Orta de Velasquez MT, Luqueño-Martinez VG, Monje-Ramirez I (2013) Quantification of Helicobacter pylori in the viable but nonculturable state by quantitative PCR in water disinfected with ozone. Water Sci Technol 68: 2468-2472.
• Azevedo, NF, Almeida C, Keevil CW, Vieira MJ (2006) Coccoid morphology as a possible manifestation of Helicobacter pylori adaptation to adverse environments. Helicobacter 11: 342-343.
• Shimoyama T (2013) Stool antigen tests for the management of Helicobacter pylori infection. World J Gastroenterol 19: 8188-8191.
• Pinto-Sanchez MI, Bercik P, Verdu EF (2015) Motility alterations in celiac disease and non-celiac gluten sensitivity. Dig Dis 33: 200-207.
• Nenna R, Magliocca FM, Tiberti C, Mastrogiorgio G, Petrarca L, Mennini M, Lucantoni F, Luparia RP, Bonamico M (2012) J Pediatr Gastroenterol Nutr 55: 728-732.
• Ferwana M, Abdulmajeed I, Alhajiahmed A, Madani W, Firwana B, Hasan R, Altayar O, Limburg PJ, Murad MH, Knawy B (2015) Accuracy of urea breath test in Helicobacter pylori infection: Meta-analysis. World J Gastroenterol 21: 1305-1314.
• Leal YA, Flores LL, Fuentes-Panana EM, Cedillo-Rivera R, Torres J (2011) 13C-urea breath test for the diagnosis of Helicobacter pylori infection in children: a systematic review and meta-analysis. Helicobacter 16: 327-337
• Zhou X, Su J, Xu G, Zhang G (2014) Accuracy of stool antigen test for the diagnosis of Helicobacter pylori infection in children: a meta-analysis. Clin Res Hepatol Gastroenterol 38: 629-638.
• Gisbert JP, de la Morena F, Abraira V (2006) Accuracy of monoclonal stool antigen test for the diagnosis of H. pylori infection: a systematic review and meta-analysis. Am J Gastroenterol 101: 1921-1930.
• Drzymała-Czyż S, Stawinska-Witoszynska B, Mądry E, Krzywinska-Wiewiórowska M, Szczepanik M, Walkowiak J, Kwiecień J (2014) Non-invasive detection of Helicobacter pylori in cystic fibrosis - the fecal test vs. the urea breath test. Eur Rev Med Pharmacol Sci 18: 2343-2348.

Identifiers

DOI

10.18388/abp.2015_1088