Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus

• Authors: Shuang Gao , Shan Ruan , Yanping Ma , Mali Li , Lin Wang , Bo Zheng , Ying Qi , Zhengrong Sun , Yujing Huang , Qiang Ruan
• Languages of publication: EN

Abstracts

EN

Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.

Keywords

EN

HCMV; UL4; UL5; transcript

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 1
• Pages: 97-101

Dates

published

2015

received

2014-06-30

revised

2014-11-12

accepted

2014-12-16

online

2015-03-02

Contributors

author

Shuang Gao

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Shan Ruan

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Yanping Ma

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Mali Li

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Lin Wang

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Bo Zheng

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Ying Qi

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Zhengrong Sun

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Yujing Huang

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

author

Qiang Ruan

• Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

References

• Adam BL, Jervey TY, Kohler CP, Wright GL Jr, Nelson JA, Stenberg RM (1995) The human cytomegalovirus UL98 gene transcription unit overlaps with the pp28 true late gene (UL99) and encodes a 58-kilodalton early protein. J Virol 69: 5304-5310.
• Chang CP, Vesole DH, Nelson J, Oldstone MB, Stinski MF (1989a) Identification and expression of a human cytomegalovirus early glycoprotein. J Virol 63: 3330-3337.
• Chang CP, Malone CL, Stinski MF (1989b) A human cytomegalovirus early gene has three inducible promoters that regulated differentially at various times after infection. J Virol 63: 281-290.
• Chee MS, Bankier AT, Beck S, Bohni R, Brown CM, Cerny R, Horsnell T, Hutchison CA 3rd, Kouzarides T, Martignetti JA, et al. (1990) Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol 154: 125-169.
• Child SJ, Hakki M, De Niro KL, Geballe AP (2004) Evasion of cellular antiviral responses by human cytomegalovirus TRS1 and IRS1. J Virol 78: 197-205.
• Davison AJ, Dolan A, Akter P, Addison C, Dargan DJ, Alcendor DJ, McGeoch DJ, Hayward GS (2003a) The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J Gen Virol 84: 17-28.
• Davison AJ, Akter P, Cunningham C, Dolan A, Addison C, Dargan DJ, Hassan-Walker AF, Emery VC, Griffiths PD (2003b) Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J Gen Virol 84: 657-663.
• Dolan A, Cunningham C, Hector RD, Hassan-Walker AF, Lee L, Addison C, Dargan DJ, McGeoch DJ, Gatherer D (2004) Genetic content of wild-type human cytomegalovirus. J Gen Virol 85: 1301-1312.
• Ma YP, Ruan Q, Ji YH, Wang N, Li ML, Qi Y, He R, Sun ZR, Ren GW (2011) Novel transcripts of human cytomegalovirus clinical strain found by cDNA library screening. Genet Mol Res 10: 566-575.
• Ma Y, Wang N, Li M, Gao S, Wang L, Zheng B, Qi Y, Ruan Q (2012) Human CMV transcripts: an overview. Future Microbiol 7: 577-593.
• Marshall EE, Bierle CJ, Brune W, Geballe AP (2009) Essential role for either TRS1 or IRS1 in human cytomegalovirus replication. J Virol 83: 4112-4120.
• Mccormick AL, Roback L, Livingston-Rosanoff D, St Clair C (2010) The human cytomegalovirus UL36 gene controls caspase- dependent and -independent cell death programs activated by infection of monocytes differentiating to macrophages. J Virol 84: 5108-5123.
• Murphy EA, Streblow DN, Nelson JA, Stinski MF (2000) The human cytomegalovirus IE86 protein can block cell cycle progression after inducing transition into the S phase of permissive cells. J Virol 74: 7108-7118.
• Murphy E, Yu D, Grimwood J, Schmutz J, Dickson M, Jarvis MA, Hahn G, Nelson JA, Myers RM (2003) Coding potential of laboratory and clinical strains of human cytomegalovirus. Proc Natl Acad Sci USA 100: 14976-14981.
• Oduro JD, Uecker R, Hagemeier C, Wiebusch L (2012) Inhibition of human cytomegalovirus immediate-early gene expression by cyclin A2-dependent kinase activity. J Virol 86: 9369-9383.
• Paulus C, Krauss S, Nevels M (2006) A human cytomegalovirus antagonist of type I IFN-dependent signal transducer and activator of transcription signaling. Proc Natl Acad Sci USA 103: 3840-3845.
• Qi Y, Ma Y, He R, Wang N, Ruan Q, Ji Y, Li M, Sun Z, Ren G (2011) Characterization of 3' termini of human cytomegalovirus UL138-UL145 transcripts in a clinical strain. Microbiol Immunol 55: 95-99.
• Skaletskaya A, Bartle LM, Chittenden T, McCormick AL, Mocarski ES, Goldmacher VS (2001) A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation. Proc Natl Acad Sci USA 98: 7829-7834.
• Spector DH (1996) Activation and regulation of human cytomegalovirus early genes. Intervirology 39: 361-377.
• Stamminger T, Puchtler E, Fleckenstein B (1991) Discordant expression of the immediate-early 1 and 2 gene regions of human cytomegalovirus at early times after infection involves posttranscriptional processing events. J Virol 65: 2273-82.
• Sun Z, Ren G, Ma Y, Wang N, Ji Y, Qi Y, Li M, He R, Ruan Q (2010) Transcription pattern of UL131A-128 mRNA in clinical strains of human cytomegalovirus. J Biosci 35: 365-370.
• Welch AR, McGregor LM, Gibson W (1991) Cytomegalovirus homologs of cellular G protein-coupled receptor genes are transcribed. J Virol 65: 3915-3918.
• Wiebusch L, Hagemeier C (2001) The human cytomegalovirus immediate early 2 protein dissociates cellular DNA synthesis from cyclin-dependent kinase activation. EMBO J 20: 1086-1098.
• Zhang G, Raghavan B, Kotur M, Cheatham J, Sedmak D, Cook C, Waldman J, Trgovcich J (2007) Antisense transcription in the human cytomegalovirus transcriptome. J Virol 81: 11267-11281.