Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 4 | 929-934

Article title

Qualitative variability in microbial community of constructed wetlands used for purifying wastewater contaminated with pharmaceutical substances


Title variants

Languages of publication



Pharmaceutical substances and their residues are increasingly present in the environment. Therefore, attempts at their removal are made by using different processes. Increasingly important among these processes are those modeled on natural phenomena which occur in wetland ecosystems, called technical scale constructed wetlands. Microbial degradation is an important process in these constructed wetlands. The biodegradation of chemicals often involves a complex series of biochemical reactions and usually varies with the microorganisms involved. The objectives of this study were to determine the impact of sulfamethoxazole and diclofenac on ammonia oxidizing bacteria and other parameters of wastewater in the microcosm of down-flow constructed wetlands. The Spearman correlation coefficient attained negative values in the case of comparison of the Shannon biodiversity index and the parameters of purified wastewater. This dependence was pronounced. In the case of pharmaceutical substances dosed with wastewater, the Spearman correlation coefficient assumed positive values. The highest value assumed by the Spearman correlation coefficient (0.9) was for the removal of diclofenac and Shannon index values for the planted columns, with a very high relationship. For unplanted columns, this value equaled 0.6. For sulfamethoxazole, the value for planted columns was 0.7, and for unplanted -0.7. The presence of plants did not have an impact on the Shannon biodiversity index.








Physical description




  • Environmental Biotechnology Department, The Silesian University of Technology, Gliwice, Poland
  • The Biotechnology Centre, The Silesian University of Technology, Gliwice, Poland
  • Environmental Biotechnology Department, The Silesian University of Technology, Gliwice, Poland
  • The Biotechnology Centre, The Silesian University of Technology, Gliwice, Poland
  • Environmental Biotechnology Department, The Silesian University of Technology, Gliwice, Poland
  • The Biotechnology Centre, The Silesian University of Technology, Gliwice, Poland


  • Cui LH, Ouyang Y, Gu WJ, Yang WZ, Xu QL (2013) Evaluation of nutrient removal efficiency and microbial enzyme activity in a baffled subsurface-flow constructed wetland system. Bioresour Technol 146: 656-662.
  • Dionisi HM, Layton AC, Harms G, Gregory IR, Robinson KG, Sayler GS (2002) Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR. Appl Environ Microbiol 68: 245-53.
  • Dong X, Reddy GB (2012) Ammonia-oxidizing bacterial community and nitrification rates in constructed wetlands treating swine wastewater. Ecol Eng 40: 189-197.
  • Faulwetter JL, Gagnon V, Sundberg C, Chazarenc F, Burr MD, Brisson J, Camper AK, Stein OR (2009) Microbial processes influencing performance of treatment wetlands: a review. Ecol Eng 35: 987-1004.
  • Hallin S, Lydmark P, Kokalj S, Hermansson M, Sorensson F, Jarvis A, Lindgren PE (2005) Community survey of ammonia-oxidizing bacteria in full-scale activated sludge processes with different solids retention time. J Appl Microbiol 99: 629-640
  • Knowles P, Dotrob G, Nivala J, García J (2011) Clogging in subsurface-flow treatment wetlands: occurrence and contributing factors. Ecol Eng 37: 99-112.
  • Kowalchuk GA, Stephen JR, De Boer W, Prosser JI, Embley TM, Woldendorp JW (1997) Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and PCR-amplified 16S ribosomal DNA fragments. Appl Environ Microbiol 63: 1489-1497.
  • Larsen E, Greenway M (2004) Quantification of biofilms in a subsurface flow wetland and their role in nutrient removal. Water Science Technology 49: 115-122.
  • Miksch K, Cema G, Corvini PF, Felis E, Sochacki A, Surmacz-Górska J, Wiszniowski J, Żabczyński S (2015) R&D priorities in the field of sustainable remediation and purification of agroindustrial and municipal wastewater. New Biotechnology 32: 128-132.
  • Muyzer G, De Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695-700.
  • Nopens I, Capalozza C, Vanrolleghem PA (2001) Stability analysis of a synthetic municipal wastewater. Department of Applied Mathematics Biometrics and Process Control. University of Gent, Belgium.
  • Nowrotek M, Sochacki A, Felis E, Miksch K (2015) Removal of diclofenac and sulfamethoxazole from synthetic municipal waste water in microcosm downflow constructed wetlands: start-up results. Int J Phytoremediation. (in press).
  • Pell M, Nyberg F (1989) Infiltration of waste-water in a newly started pilot sand-filter system. 2. Development and distribution of the bacterial-populations. J Environ Qual 18: 457-462.
  • Pester M, Rattei T, Flechl S, Gröngröft A, Richter A, Overmann J, Reinhold-Hurek B, Loy A, Wagner M (2012) AmoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions. Environ Microbiol 14: 525-539.
  • Saeed T, Sun G (2012) A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands: dependency on environmental parameters, operating conditions and supporting media. J Environ Manage 112: 429-448.
  • Sochacki A, Faure O, Guy B, Surmacz-Górska J (2015) Polishing of Real Electroplating Wastewater in Microcosm Fill-and-Drain Constructed Wetlands. In The Role of Natural and Constructed Wetlands in Nutrient Cycling and Retention on the Landscape, Vymazal J eds, Springer International Publishing Switzerland.
  • StatSoft, Inc. (2011) STATISTICA (data analysis software system), version 10. www.statsoft.com.
  • Sundberg C, Stendahl JSK, Tonderski K, Lindgren PE (2007a) Overland flow systems for treatment of landfill leachates - potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season. Soil Biol Biochem 39: 127-38.
  • Tietz A, Kirschner A, Langergraber G, Sleytr K, Haberl R (2007) Characterisation of microbial biocoenosis in vertical subsurface flow constructed wetlands. Sci Total Environ 380: 163-172.
  • Truu M, Juhanson J, Truu J (2009) Microbial biomass, activity and community composition in constructed wetlands. Sci Total Environ 407: 3958-3971.
  • Watnick P, Kolter R (2000) Biofilm, city of microbes. J Bacteriol 182: 2675-2679.
  • Wu S, Kuschk P, Brix H, Vymazal J, Dong R (2014) Development of constructed wetlands in performance intensifications for wastewater treatment: A nitrogen and organic matter targeted review. Water Research 57: 40-55.
  • Wu YH, Li TL, Yang LZ (2012) Mechanisms of removing pollutants from aqueous solutions by microorganisms and their aggregates: a review. Bioresour Technol 107: 10-18.

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.