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2015 | 62 | 4 | 913-921
Article title

The balance between pro- and anti-inflammatory cytokines in the immune responses to BCG and DTwP vaccines

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EN
Abstracts
EN
Bacillus Calmette-Guérin (BCG) and pertussis vaccines have been found to be insufficient and their further improvement is required. In order to develop improved vaccines, a better understanding of the main pathways involved in the host's protective immunity to the pathogens is crucial. We address the question as to whether the balance between pro- and anti-inflammatory cytokine production might affect the host responses to BCG and diphtheria-tetanus toxoids-whole cell pertussis (DTwP) vaccines. The study population consisted of 118 healthy people, age range 18-30 years, who had been subjected to BCG and DTwP vaccination according to the state policy. Tuberculin skin testing (TST) revealed a delayed type hypersensitivity (DTH) to PPD (purified protein derivative) in 53% volunteers. The variability in development of the BCG-driven DTH to tuberculin prompted us to address a question as to whether Th1/Th2 polarization is involved in the lack of skin responsiveness to PPD. PPD-stimulated blood lymphocytes from TST+ participants produced significantly more IFN-γ and less IL-10 than lymphocytes from TST- volunteers. However, TST- volunteers' sera contained more anti-pertussis IgG but not anti-diphtheria toxin IgG. Mycobacterial antigens and particularly PPD induced a higher expression of HLA-DR and co-stimulatory CD80 receptors on DCs from TST+ than TST- participants. BCG but not PPD pulsed DCs from TST- volunteers produced significantly more IL-10. Mycobacterial antigen stimulated DCs from TST+ volunteers induced a more intense IFN-γ production in co-cultures with autologous lymphocytes than the cells from TST- participants. Differences among the types of dendritic cell activities contribute to development of tuberculin reactivity in BCG vaccinated volunteers.
Publisher

Year
Volume
62
Issue
4
Pages
913-921
Physical description
Dates
published
2015
received
2015-07-31
revised
2015-10-02
accepted
2015-10-27
(unknown)
2015-12-07
Contributors
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Division of Cellular Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
References
  • Balfour BM, Goscicka T, MacKenzie JL, Gautam A, Tate M, Clark J (1990) Combined time-lapse cinematography and immuno-electron microscopy. Anat Rec 226: 509-514.
  • Bedford PA, Burke F, Stagg AJ, Knight SC (2008) Dendritic cells derived from bone marrow cells fail to acquire and present major histocompatibility complex antigens from other dendritic cells. Immunology 124: 542-552.
  • Blood-Siegfried J, Crabb Breen E, Takeshita S, Martinez-Maza O, Vredevoe D (1998) Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components. J Clin Immunol 18: 81-88.
  • Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K (2012) Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations. J Invest Dermatol 132: 114-123.
  • Brewer TF, Colditz GA (1995) Relationship between Bacille Calmette-Guérin (BCG) strains and the efficacy of BCG vaccine in the prevention of tuberculosis. Clin Infect Dis 20: 126-135.
  • Cottrez F, Hurst SD, Coffman RL, Groux H (2000) T regulatory cells 1 inhibit Th2-specific response in vivo. J Immunol 165: 4848-4853.
  • Delgado JC, Tsai EY, Baena A, Boussiotis VA, Reynes JM, Sath S, Grosjean P, Yunis EJ, Goldfeld AE (2002) Antigen-specific and persistent tuberculin anergy in a cohort of pulmonary tuberculosis patients from rural Cambodia. Proc Natl Acad Sci USA 99: 7576-7581.
  • Feng CG, Kullberg MC, Jankovic D, Cheever AW, Caspar P, Coffman RL, Sher A (2002) Transgenic mice expressing human interleukin-10 in the antigen-presenting cell compartment show increased susceptibility to infection with Mycobacterium avium associated with decreased macrophage effector function and apoptosis. Infect Immun 70: 6672-6679.
  • Fiett J, Letowska I, Gniadkowski M, Hryniewicz W (2003) The new strategy for allele identification of the genes coding for pertussis subunit S1 (ptx S1) and pertactin (prn) in Bordetella pertussis. J Microbiol Methods 55: 651-666.
  • Flynn JL, Chan J (2001) Immunology of tuberculosis. Annu Rev Immunol 19: 93-129.
  • Fingermann M, Fernández J, Sisti F, Rodriguez ME, Gatti B, Bottero D, Graieb A, Gaillard ME, Ayala SG, Mooi FR, Lopardo H, Hozbor D (2006) Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production. Vaccine 24: 3513-3521.
  • Gzyl A, Augustynowicz E, Gniadek G, Rabczenko D, Dulny G, Lusarczyk J (2004) Sequence variation in pertussis S1 subunit toxin and pertussis gene in Bordetella pertussis strains used for the whole-cell pertussis vaccine produced in Poland since 1960. Efficiency of the DTwP vaccine-induced immunity against currently circulating B. pertussis isolates. Vaccine 22: 2122-2128.
  • Hardwick TH, Cassiday P, Weyant RS, Bisgard KM, Sanden GN (2002) Changes in predominance and diversity of genomic subtypes of Bordetella pertussis isolates in the United States, 1935 to 1999. Emerg Infect Dis 8: 44-49.
  • Henderson RA, Watkins SC, Flynn JL (1997) Activation of human dendritic cells following interaction with Mycobacterium tuberculosis. J Immunol 159: 635-643.
  • Jepson A, Fowler A, Banya W, Singh M, Bennett S, Whittle H, Hill AV (2001) Genetic regulation of acquired immune responses to antigens of Mycobacterium tuberculosis: a study of twins in West Africa. Infect Immun 69: 3989-3994.
  • Kaufmann SH (2013) Tuberculosis vaccines: Time to think about the next generation. Semin Immunol 25: 172-181.
  • Kelly P, McKeown D, Clancy L (1997) Neonatal BCG vaccination in Ireland: evidence of its efficacy in the prevention of childhood tuberculosis. Eur Respir J 10: 619-623.
  • Kowalewicz-Kulbat M, Kaźmierczak D, Donevski D, Biet F, Pestel J, Rudnicka W (2008) Naive helper T cells from BCG-vaccinated volunteers produce IFN-g and IL-5 to mycobacterial antigen-pulsed dendritic cells. Folia Histochem Cytobiol 46: 153-157.
  • de Melker HE, Conyn-van Spaendonck MA, Rumke HC, van Wijngaarden JK, Mooi FR, Schellekens JF (1997) Pertussis in the Netherlands: an outbreak despite high levels of immunization with whole cell vaccine. Emerg Infect Dis 3: 175-178.
  • Mollenkopf HJ, Kursar M, Kaufmann SH (2004) Immune response to postprimary tuberculosis in mice: Mycobacterium tuberculosis and Mycobacterium bovis bacille Calmette-Guerin induce equal protection. J Infect Dis 190: 588-597.
  • Olin P, Gustafsson L, Barreto L, Hessel L, Mast TC, Rie AV, Bogaerts H, Storsaeter J (2003) Declining pertussis incidence in Sweden following the introduction of acellular pertussis vaccine. Vaccine 21: 2015-2021.
  • Paziak-Domańska B, Bonar A, Kowalewicz-Kulbat M, Klink M, Kowalski M, Karhukorpi J, Karttunen R, Jurkiewicz M, Rózalska B, Rudnicka W (2002) The lack of relationship between serum content of MBL, sCD14, anti-PPD and anti-Hsp65 IgG and ingestion of Mycobacterium bovis BCG bacilli by phagocytes. Arch Immunol Ther Exp (Warsz) 50: 337-344.
  • Powell DA, Hunt WG (2006) Tuberculosis in children: an update. Adv Pediatr 53: 279-322.
  • Rengarajan J, Szabo SJ, Glimcher LH (2000) Transcriptional regulation of Th1/Th2 polarization. Immunol Today 21: 479-83.
  • de Serres G, Boulianne N, Douville Fradet M, Duval B (1995) Pertussis in Quebec: ongoing epidemic since the late. Can Commun Dis Rep 21: 45-48.
  • Steinman RM (2008) Dendritic cells in vivo: a key target for a new vaccine science. Immunity 29: 319-324.
  • Szpakowski P, Biet F, Locht C, Paszkiewicz M, Rudnicka W, Druszczynska M, Allain F, Fol M, Pestel J, Kowalewicz-Kulbat M (2015) Dendritic cell activity driven by recombinant Mycobacterium bovis BCG producing human IL-18, in healthy BCG vaccinated adults. J Immunol Res 2015: 359153.
  • Tala-Heikkila MM, Tuominen JE, Tala EO (1998) Bacillus Calmette-Guérin revaccination questionable with low tuberculosis incidence. Am J Respir Crit Care Med 157: 1324-1327.
  • Tan T, Trindade E, Skowronski D (2005) Epidemiology of pertussis. Pediatr Infect Dis J 24 (Suppl 5): S10-S8.
  • Thurner B, Roder C, Dieckmann D, Heuer M, Kruse M, Glaser A, Keikavoussi P, Kämpgen E, Bender A, Schuler G (1999) Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application. J Immunol Methods 223: 1-15.
  • World Health Organization.org [Internet]. Global Tuberculosis Report 2014. Available from:
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p913kz
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