The impact of polyphenols on Bifidobacterium growth
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Polyphenols are a common group of plant based bioactive compounds, that can affect human health because of their antioxidant and antimicrobial properties as well as free-radical scavenging activity. An increasing interest is observed in the interaction between polyphenols and microbiota occurring in food and the human gut. The aim of the work presented here, was to evaluate the effect of some polyphenolic compounds on the growth of two strains of Bifidobacterium: B. adolescentis and B. bifidum. The influence of some flavonoids: naringinin, hesperidin, rutin, quercetin as well as phenolic acids: gallic, caffeic, p-coumaric, ferulic, chlorogenic, vanillic and sinapic was determined by a 96-well microtiter plate assay. In the experiments the effect of three different concentrations of polyphenols: 2, 20 and 100 µg/ml on the growth of Bifidobacterium strains was investigated. All tested compounds influenced the growth of the examined bacteria. Both stimulatory and inhibitory effects were observed in comparison to the positive control. The strongest impact on the growth of bifidobacteria was observed during the first hours of incubation. The constant inhibitory effect was observed for hesperidin and quercetin addition and was dose-dependent. B. bifidum showed a stronger dependence on phenolic acids content in the medium than B. adolescentis during the first hours of incubation.
- Adlercreutz H, Mazur W (1997) Phyto-oestrogens and Western diseases. Ann Med 29: 95-120.
- Ahn YJ, Kawamura T, Kim M, Yamamoto T, Mitsuoka T (1991) Tea polyphenols: selective growth inhibitors of Clostridium spp. Agric Biol Chem 55: 1425-1426.
- Andrés-Lacueva C, Medina-Remon A, Llorach R, Urpi-Sarda M, Khan N, Chiva-Blanch G, Zamora-Ros R, Rotches-Ribalta M, Lamuela-Raventos RM (2009) Phenolic compounds: chemistry and occurrence in fruits and vegetables. In Fruit and Vegetable Phytochemicals: Chemistry, Nutritional Value and Stability. de la Rosa LA, Álvarez-Parrilla E, González-Aguilar GA eds, pp 53-88. Blackwel Publishing, Ames, IA.
- Arts IC, Hollman PC (2005) Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 81 (1 Suppl): 317S-325S.
- Commenges D, Scotet V, Renaud S, Jacqmin-Gadda H, Barberger-Gateau P, Dartigues JF (2000) Intake of flavonoids and risk of dementia. Eur J Epidemiol 16: 357-363.
- Cushnie TP, Hamilton VE, Chapman DG, Taylor PW, Lamb AJ (2007) Aggregation of Staphylococcus aureus following treatment with the antibacterial flavonolgalangin. J Appl Microbiol 103: 1562-1567.
- D'Archivio M, Filesi C, Di Benedetto R, Gargiulo R, Giovannini C, Masella R (2007) Polyphenols, dietary sources and bioavailability. Ann Ist Super Sanita 43: 348-361.
- Daglia M (2012) Polyphenols as antimicrobial agents. Curr Opin Biotechnol 23: 174-181.
- Diker KS, Akan M, Hascelik G, Yurdakok M (1991) The bacterial activity of tea against Campylobacter jejuni and Campylobacter coli. Lett Appl Microbiol 12: 34-35.
- Dolara P, Luceri C, De Filippo C, Femia AP, Giovannelli L, Caderni G, Cecchini C, Silvi S, Orpianesi C, Cresci A (2005) Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats. Mutat Res 591: 237-246.
- Duda-Chodak A (2012) The inhibitory effect of polyphenols on human gut microbiota. J Physiol Pharmacol 63: 497-503.
- Graf BA, Milbury PE, Blumberg JB (2005) Flavonols, flavones, flavanones, and human health: Epidemological evidence. J Med Food 8: 281-90.
- Isogai E, Isogai H, Takeshi K, Nishikawa T (1998) Protective effect of Japanese green tea extract on gnotobiotic mice infected with an Escherichia coli O157:H7 strain. Microbiol Immunol 42: 125-128.
- Larrosa M, Yañéz-Gascón MJ, Selma MV, González-Sarrías A, Toti S, Cerón JJ, Tomas-Barberán F, Dolara P, Espín JC (2009) Effect of a low dose of dietary resveratrol on colon microbiota, inflammation and tissue damage in a DSS-induced colitis rat model. J Agric Food Chem 57: 2211-2220.
- Lee HC, Jenner AM, Low CS, Lee YK (2006) Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota. Res Microbiol 157: 876-884.
- Letenneur L, Proust-Lima C, Le Gouge A, Dartigues JF, Barberger-Gateau P (2007) Flavonoid intake and cognitive decline over a 10-year period. Am J Epidemiol 165: 1364-1371.
- Margolles A, Ruas-Madiedo P, de los Reyes-Gavilán CG, Sánchez B, Gueimonde M (2011) Bifidobacterium. In Molecular Detection of human bacterial pathogens Liu D, eds, pp 45-57. Florida: CRC Press, Taylor & Francis Group.
- Marsilio V, Lanza B (1998) Characterization of an oleuropein degrading strain of Lactobacillus plantarum. Combined effects of compounds present in olive fermenting brines (phenols, glucose and NaCl) on bacterial activity. J Sci Food Agric 76: 520-524.
- Masco L, Huys G, De Brandt E, Temmerman R, Swings J (2005) Culture-dependent and culture-independent qualitative analysis of probiotic products claimed to contain bifidobacteria. Int J Food Microbiol 102: 221-230.
- Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79: 727-747.
- Parr AJ, Bolwell GP (2000) Phenols in the plant and in man The potential for possible nutritional enhancement of the diet by modifying the phenol content or profile. J Agric Food Chem 80: 985-1012.
- Rozès N, Peres C (1998) Effects of phenolic compounds on the growth and the fatty acid composition of Lactobacillus plantarum. Appl Microbiol Biotechnol 49: 108-111.
- Quideau S, Deffieux D, Douat-Casassus C, Pouysegu L (2011) Plant polyphenols: chemical properties, biological activities, and Quideau synthesis. Angew Chem Int Ed Engl 50: 586-621.
- Saavedra MJ, Borges A, Dias C, Aires A, Bennett RN, Rosa ES, Simoes M (2010) Antimicrobial activity of phenolics and glucosinolate hydrolysis products and their synergy with streptomycin against pathogenic bacteria. Med Chem 6: 174-183.
- Sakanaka SN, Shimura M, Aizawa MK, Yamamoto T (1992) Preventive effect of green tea polyphenols against dental caries in conventional rats. Biosci Biotechnol Biochem 56: 592-594.
- Sánchez-Patán F, Cueva C, Monagas M, Walton GE, Gibson GR, Quintanilla-López JE, Lebrón-Aguilar R, Martín-Álvarez PJ, Moreno-Arribas MV, Bartolomé B (2012) In Vitro Fermentation of a Red Wine Extract by Human Gut Microbiota: Changes in Microbial Groups and Formation of Phenolic Metabolites. J Agric Food Chem 60: 2136-2147.
- Sanoner P, Guyot S, Marnet N, Molle D, Drilleau JF (1999) Polyphenol profiles of French cider apple varieties (Malusdomestica sp.). J Agric Food Chem 47: 4847-53.
- Salih AG, Le Quéré JM, Drilleau JF (2000) Action des acides hydroxycinnamiques libres et esterifies sur la croissance des bactéries lactiques. Sci des Aliments 20: 537-560.
- Stead D (1993) The effect of hydroxycinnamic acids on the growth of wine spoilage lactic acid bacteria. J Appl Bacteriol 75: 135-141.
- Stead D (1994) The effect of chlorogenic, gallic and quinic acids on the growth of spoilage strains of Lactobacillus collinoides and Lactobacillus brevis. Lett Appl Microbiol 18: 112-114.
- Tojo R, Suárez A, Clemente MG, de los Reyes-Gavilán CG, Margolles A, Gueimonde M, Ruas-Madiedo P (2014) Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis. World J Gastroenterol 20: 15163-15176.
- Turroni F, Marchesi JR, Foroni E, Gueimonde M, Shanahan F, Margolles A, van Sinderen D, Ventura M (2009) Microbiomic analysis of the bifidobacterial population in the human distal gut. ISME J 3: 745-751.
- Tzounis X, Vulevic J, Kuhnle GG, George T, Leonczak J, Gibson GR, Kwik-Uribe C, Spencer JP (2008) Flavanol monomer-induced changes to the human faecal microflora. Br J Nutr 99: 782-92.
- Yamakoshi J, Tokutake S, Kikuchi M, Kubota Y, Konishi H, Mitsuoka T (2001) Effect of proanthocyanidin-rich extract from grape seeds on human fecal flora and fecal odor. Microb Ecol Health Dis 13: 25-31.
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