PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 4 | 879-883
Article title

Monomeric and gemini surfactants as antimicrobial agents - influence on environmental and reference strains

Content
Title variants
Languages of publication
EN
Abstracts
EN
Quaternary ammonium salts (QAS) belong to surfactant commonly used both, in the household and in different branches of industry, primarily in the process of cleaning and disinfection. They have several positive features inter alia effectively limiting the development of microorganisms on many surfaces. In the present work, two compounds were used as biocides: hexamethylene-1,6-bis-(N,N-dimethyl-N-dodecylammonium bromide) that belongs to the gemini surfactant (GS), and its single analogue - dodecyl(trimethyl)ammonium bromide (DTAB). Two fold dilution method was used to determine the minimum concentration of compounds (MIC) which inhibit the growth of bacteria: Staphylococcus aureus (ATCC 6538 and an environmental strain), Pseudomonas aeruginosa (ATCC 85327 and an environmental strain), and yeast Candida albicans (ATCC 11509 and an environmental strain). The viability of cells in liquid cultures with addition of these substances at ¼ MIC, ½ MIC and MIC concentrations were also determined. The obtained results show that DTAB inhibits the growth of bacteria at the concentration of 0.126-1.010 µM/ml, and gemini surfactant is active at 0.036-0.029 µM/ml. Therefore, GS is active at more than 17-70-fold lower concentrations than its monomeric analogue. Strains isolated from natural environment are less sensitive upon testing biocides than the references strains. Both compounds at the MIC value reduced the number of cells of all strains. The use of too low concentration of biocides can limit the growth of microorganisms, but often only for a short period of time in case of special environmental strains. Later on, they can adapt to adverse environmental conditions and begin to evolve defence mechanisms.
Year
Volume
62
Issue
4
Pages
879-883
Physical description
Dates
published
2015
received
2015-08-01
revised
2015-10-13
accepted
2015-10-30
(unknown)
2015-12-07
References
  • Aka ST, Haji SH (2015) Sub-MIC of antibiotics induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine. Braz J Microbiol 46: 149-154.
  • Brycki B, Kowalczyk I, Koziróg A (2011) Synthesis, molecular structure, spectral properties and antifungal activity of polymethylene-α,ω-bis(N,N-dimethyl-N-dodecyloammonium bromides) Molecules 16: 319-335.
  • Castillo JA, Clapés P, Infante MR, Comas J, Manresa A (2006) Comparative study of the antimicrobial activity of bis(Nα-caproyl-l-arginine)-1,3-propanediamine dihydrochloride and chlorhexidine dihydrochloride against Staphylococcus aureus and Escherichia coli. J Antimicrob Chemother 57: 691-698.
  • Clinical and Laboratory Standards Institute (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - Ninth Edn. CLSI document M07-A9. Wayne, PA.
  • Dong L, Tong Z, Linghu D, Lin Y, Tao R, Liu J, Tian Y, Ni L (2012) Effects of sub-minimum inhibitory concentrations of antimicrobial agents on Streptococcus mutans biofilm formation. Int J Antimicrob Agents 39: 390-395.
  • Gutarowska B, Skóra J, Pietrzak K, Rembisz D (2012) Analysis of the sensitivity of microorganisms contaminating museums and archives to silver nanoparticles. Int Biodeterior Biodegradation 68: 7-17.
  • Hait SK, Moulik SP (2002) Gemini surfactants: a distinct class of self-assembling molecules. Curr Sci 82: 1101-1111.
  • Hegstad K, Langsrud S, Lunestad BT, Scheie AA, Sunde M, Yazdankhah SP (2010) Does the wide use of quaternary ammonium compounds enhance the selection and spread of antimicrobial resistance and thus threaten our health? Microb Drug Resist 16: 91-104.
  • Koziróg A, Brycki B, Nowak A (2015) Influence of surfactants on environmental and collection bacterial strains. Acta Bioch Pol Suppl. 2: 132.
  • Lambert RJ, Joynson J, Forbes B (2001) The relationships and susceptibilities of some industrial, laboratory and clinical isolates of Pseudomonas aeruginosa to some antibiotics and biocides. J Appl Microbiol 91: 972-984.
  • Menger FM, Keiper JS (2000) Gemini surfactants. Angew Chem Int Ed Engl 39: 1906-1920.
  • Obłąk E, Piecuch A, Guz-Regner K, Dworniczek E (2014) Antibacterial activity of gemini quaternary ammonium salts. FEMS Microbiol Lett 350: 190-198.
  • PN-EN 1276: 2010. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants andantiseptics used in food, industrial, domestic and institutional areas - Test method and requirements (phase 2, step 1).
  • PN-EN 1650 +A1: 2013-08. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas - Test method and requirements (phase 2, step 1).
  • Raghavendra T (2002) Neuromuscular blocking drugs: discovery and development. J R Soc Med 95: 363-367
  • Rucka M, Oświęcimska M, Witek S (1983) New biocides for cooling water treatment. III Quaternary ammonium salts. Env Protection Eng 6: 455-463.
  • Russell AD (2003) Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations. Lancet Infect Dis 3: 794-803.
  • Sekhon BS (2004) Gemini (dimeric) surfactants. Resonance 9: 42-49.
  • Shirai A, Sumitomo T, Yoshida M, Kaimura T, Nagamune H, Maeda T, Kourai H (2006) Synthesis and biological properties of gemini quaternary ammonium compounds, 5,5'-[2,2'-(α,ω-polymethylnedicarbonyldioxy)diethyl]bis-(3-alkyl-4-methylthiazolium iodide) and 5,5'-[2,2'-(p-phenylenedi-carbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium bromide) Chem Pharm Bull 54: 639-645.
  • Shukla D, Tyagi VK (2006) Cationic gemini surfactants: a review. J Oleo Sci 55: 381-390.
  • Thomas L, Maillard JY, Lambert RJ, Russell AD (2000) Development of resistance to chlorhexidine diacetate in Pseudomonas aeruginosa and the effect of a 'residual' concentration. J Hosp Infect 46: 297-303.
  • Wojcicka L, Hofmann R, Baxter C, Andrews RC, Auvray I, Lière J, Miller T, Chauret Ch, Baribeau H (2007) Inactivation of environmental and reference strains of heterotrophic bacteria and Escherichia coli O157:H7 by free chlorine and monochloramine. J Water Supply Res T 56: 137-150.
  • Vieira M, Pinheiro C, Fernandes R, Noronha JP, Prudêncio C (2014) Antimicrobial activity of quinoxaline 1,4-dioxide with 2- and 3-substituted derivatives. Microbiol Res 169: 287-293.
  • Xiao YH, Chen JH, Fang M, Xing XD, Wang H, Wang YJ, Li F (2008) Antibacterial effects of three experimental quaternary ammonium salt (QAS) monomers on bacteria associated with oral infections. J Oral Sci 50: 323-327.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p879kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.