Biological activity of surfactins - a case of a biosurfactant produced by Bacillus subtilis PCM 1949

• Authors: Emilia Siwak , Michał Jewgiński , Irena Kustrzeba-Wójcicka
• Languages of publication: EN

Abstracts

EN

Biosurfactants are microbial surface active compounds which, contrary to synthetic surfactants, are natural in origin, biodegradable and less toxic to a human organism. For that reason, there is a great research potential in studies aimed at their purification, finding potential ways of their utilization and decreasing their production costs. This paper demonstrates the process of isolating and purifying a surfactin synthesized by Bacillus subtilis PCM 1949. Surfactin samples were prepared by a classical organic solvent extraction and were studied using mass spectrometry (MS). Analysis of the susceptibility profile of microorganisms utilized in the diffusion-plate tests demonstrated that their sensitivity to this surfactin is differentiated and depends on the microorganism species. In our studies, we found that the selected strains of bacteria and fungi were insensitive to this surfactin at a wide range of concentrations.

Keywords

EN

surfactin; Bacillus subtilis; biosurfactants; biological activity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 875-878

Dates

published

2015

received

2015-07-31

revised

2015-10-27

accepted

2015-11-23

(unknown)

2015-12-04

Contributors

author

Emilia Siwak

• Department of Medical Biochemistry, Wrocław Medical University, Wrocław, Poland

author

Michał Jewgiński

• Department of Bioorganic Chemistry, Wrocław University of Technology, Wrocław, Poland

author

Irena Kustrzeba-Wójcicka

• Department of Medical Biochemistry, Wrocław Medical University, Wrocław, Poland

References

• Abdel-Mawgoud AM, Aboulwafa MM, Hassouna NA (2008) Optimization of surfactin production by Bacillus subtilis isolate BS5. Appl Biochem Biotechnol 150: 305-325.
• Arima K, Kakinuma A, Tamura G (1968) Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: Isolation, characterization and its inhibition of fibrin clot formation. Biochem Biophys Res Commun 31: 488-494.
• Cao XH, Wang AH, Wang CL, Mao DZ, Lu MF, Cui YQ, Jiao RZ (2010) Surfactin induces apoptosis in human breast cancer MCF-7 cells through a ROS/JNK-mediated mitochondrial/caspase pathway. Chem Biol Interact 183: 357-362.
• Chen H, Juang R (2008) Recovery and separation of surfactin from pretreated fermentaton broths by physical and chemical extraction. Biochem Eng J 38: 39-46.
• Duarte C, Gudiña EJ, Lima CF, Rodrigues LR (2014) Effects of biosurfactants on the viability and proliferation of human breast cancer cells. AMB Express 4: 40.
• Gudiña EJ, Rangarajan V, Sen R, Rodrigues LR (2013) Potential therapeutic applications of biosurfactants. Trends Pharmacol Sci 34: 667-675.
• Kowall M, Vater J, Kluge B, Stein T, Franke P, Ziessow D (1998) Separation and characterization of surfactin isoforms produced by Bacillus subtilis OKB 105. J Colloid Interface Sci 204: 1-8.
• Kracht M, Rokos H, Ozel M, Kowall M, Pauli G, Vater J (1999) Antiviral and hemolytic activities of surfactin isoforms and their methyl ester derivatives. J Antibiot 52: 613-619.
• Krasowska A (2010) Biomedial activity of biosurfactants. Postepy Hig Med Dosw (Online) (online) 64: 310-313 (in Polish).
• Mnif I, Ghribi D (2015) Review lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications. Biopolymers 104: 129-147.
• Nagórska K, Bikowski M, Obuchowski M (2007) Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochim Pol 54: 495-508.
• Seydlová G, Svobodová J (2008) Review of surfactin chemical properties and the potential biomedical applications. Cent Eur J Med 3: 123-133.
• Shaligram NS, Singhal RS (2010) Surfactin - a review on biosynthesis, fermentation, purification and applications. Food Technol Biotechnol 48: 119-134.
• Singh P, Cameotra SS (2004) Potential applications of microbial surfactants in biomedical sciences. Trends Biotechnol 22: 142-146.
• Stein T (2005) Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56: 845-857.
• Vollenbroich D, Ozel M, Vater J, Kamp RM, Pauli G (1997) Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilisi. Biologicals 25: 289-297.

Identifiers

DOI

10.18388/abp.2015_1149