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2015 | 62 | 4 | 851-857
Article title

Influence of silver nanoparticles on metabolism and toxicity of moulds

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The unique antimicrobial features of silver nanoparticles (AgNPs) are commonly applied in innumerable products. The lack of published studies on the mechanisms of AgNPs action on fungi resulted in identification of the aim of this study, which was: the determination of the influence of AgNPs on the mould cytotoxicity for swine kidney cells (MTT test) and the production of selected mycotoxins, organic acids, extracellular enzymes by moulds. The conducted study had shown that silver nanoparticles can change the metabolism and toxicity of moulds. AgNPs decrease the mycotoxin production of Aspergillus sp. (81-96%) and reduce mould cytotoxicity (50-75%). AgNPs influence the organic acid production of A. niger and P. chrysogenum by decreasing their concentration (especially of the oxalic and citric acid). Also, a change in the extracellular enzyme profile of A. niger and P. chrysogenum was observed, however, the total enzymatic activity was increased.
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  • Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland
  • Institute of Experimental Biology, Kazimierz Wielki University, Bydgoszcz, Poland
  • Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland
  • Institute of Experimental Biology, Kazimierz Wielki University, Bydgoszcz, Poland
  • Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland
  • Al-Othman MR, Abd El-Aziz ARM, Mahmoud MA, Fifan SA, El-Shikh, Majrashi M (2014) Application of silver nanoparticles as antifungal and antiaflatoxin B1 produced by Aspergillus flavus. Dig J Nanomater Bios 9: 151-157.
  • Applegate KL, Chipley JR (1973) Increased aflatoxin G1 production by Aspergillus flavus via gamma irradiation. Mycologia 65: 1266-1273.
  • Aziz NH, Shahin AA, Abou-Zeid AA, El-Zeany SA (2000) Correlation of growth and aflatoxin production by Aspergillus flavus with some essential metals in gamma irradiated crushed corn. Nahrung 44: 354-359.
  • Blumenthal CZ (2004) Production of toxic metabolites in Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi. Regul Toxicol Pharmacol 39: 214-228.
  • Bokhari F, Mohammad Aly M (2009) Trials towards reduction of fungal growth and aflatoxin G1 production in Arabic coffee using different additives. Afr J Food Sci 3: 068-076.
  • Bragg PD, Rainnie DJ (1974) The effect of silver ions on the respiratory chain of Escherichia coli. Can J Microbiol 20: 883-889.
  • Braydich-Stolle LK, Lucas B, Schrand A, Murdock RC, Lee T, Schlager JJ, Hussain SM, Hofmann MC (2010) Silver nanoparticles disrupt GDNF/Fyn kinase signalling in spermatogonial stem cells. Toxicol Sci 116: 577-589.
  • Coulibaly L, Agathos SN (2007) Effects of Aspergillus niger inoculum concentration upon the kinetics of starchy wastewater pretreatment in a tanks-in-series bioreactor under transitory conditions. Braz J Chem Eng 24: 499-507.
  • Cunningham JE, Kuiack C (1992) Production of citric and oxalic acids and solubilisation. Appl Environ Microbiol 58: 1451-1458.
  • Dallas P, Sharma VK, Zboril R (2011) Silver polymeric nanocomposites as advanced antimicrobial agents: Classification, synthetic paths, applications, and perspectives. Adv Colloid Interface Sci 166: 119-135.
  • Davis ND, Diener UL, Eldridge DW (1966) Production of Aflatoxins B1 and G1 by Aspergillus flavus in a Semisynthetic Medium. Appl Microbiol 14: 378-380.
  • DiRienzo M. (2006) New applications for silver. The LBMA Precious Metals Conference, Montreux.
  • Egger S, Lehmann RP, Height MJ, Loessner MJ, Schuppler M (2009) Antimicrobial properties of a novel silver-silica nanocomposite material. Appl Environ Microbiol 75: 2973-2976.
  • El-Desouky TA, Sharoba AMA, El-Desouky AI, El-Mansy HA, Naguib K (2012) Effect of ozone gas on degradation of Aflatoxin B1 and Aspergillus flavus fungal. J Environ Anal Toxicol 2: 128.
  • Endo M, Takesako K, Kato I, Yamaguchi H (1997) Fungicidal action of aureobasidin A, a cyclic depsipeptide antifungal antibiotic, against Saccharomyces cerevisiae. Antimicrob Agents Chemother 41: 672.
  • Fakruddin M, Chowdhury A, Hossain MN, Ahmed MM (2015) Characterization of aflatoxin producing Aspergillus flavus from food and feed samples. SpringerPlus 4: 159.
  • Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52: 662-668.
  • Frisvad JC, Smedsgaard J, Samson RA, Larsen TO, Thrane U (2007) Fumonisin B2 Production by Aspergillus niger. J Agric Food Chem 55: 9727-9732.
  • Frisvad JC, Larsen TO, Thrane U, Meijer M, Varga J, Samson RA, Nielsen KF (2011) Fumonisin and Ochratoxin Production in Industrial Aspergillus niger Strains. PLoS ONE 6: e23496.
  • Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M (2009) Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine 5: 382-386.
  • Gil-Serna J, Patiño B, Cortés L, González-Jaén MT, Vázquez C (2011) Mechanisms involved in reduction of ochratoxin A produced by Aspergillus westerdijkiae using Debaryomyces hansenii CYC 1244. Int J Food Microbiol 151: 113-118.
  • Gniadek A, Macura AB, Górkiewicz M (2011) Cytotoxicity of Aspergillus fungi isolated from hospital environment. Pol J Microbiol 60: 59-63.
  • Gogoi SK, Gopinath P, Paul A, Ramesh A, Ghosh SS, Chattopadhyay (2006) Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir 22: 9322-9328.
  • Gutarowska B (2010) Metabolic activity of moulds as a factor of building materials biodegradation. Pol J Microbiol 59: 119-124.
  • Gutarowska B, Skóra J, Zduniak K, Rembisz D (2012a) Analysis of the sensitivity of microorganisms contaminating museums and archives to silver nanoparticles. Int Biodeterior Biodegradation 68: 7-17.
  • Gutarowska B, Rembisz D, Zduniak K, Skóra J, Szynkowska M, Gliścińska E, Koziróg A (2012b) Optimization and application of the misting method with silver nanoparticles for disinfection of the historical objects. Int Biodeterior Biodegradation 75: 167-175.
  • Gutarowska B, Pietrzak K, Machnowski W, Danielewicz D, Szynkowska M, Konca P, Surma-Ślusarska B (2014a) Application of silver nanoparticles for disinfection of materials to protect historical objects. Curr Nanosci 10: 277-286.
  • Gutarowska B, Skóra J, Stępień Ł, Twarużek M, Błajet-Kosicka A, Otlewska A, Grajewski J (2014b) Estimation of fungal contamination and mycotoxin production at workplaces in composting plants, tanneries, archives and libraries. World Mycotoxin J 7: 345-355.
  • Hanelt M, Gareis M, Kollarczik B (1994) Cytotoxicity of mycotoxins evaluated by the MTT-cell culture assay. Mycopathologia 128: 167-174.
  • Hassan AA, Howayda ME, Mahmoud HH (2013) Effect of Zinc Oxide Nanoparticles on the Growth of Mycotoxigenic Mould. Studies in Chemical Process Technology 1: 66-74.
  • Holt KB, Bard AJ (2005) Interaction of silver(I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. Biochemistry 44: 13214-13223.
  • Huo S, Xue X, Li Q, Xu S, Cai W (2006) Seeded-growth approach to fabrication of silver nanoparticle films on silicon for electrochemical surface-enhanced IR absorption spectroscopy. J Phys Chem B 110: 25721-25728.
  • Janda K, Ulfig K, Markowska-Szczupak A (2009) Further studies of extracellular enzyme profiles of xerophilic fungi isolates from dried medicinal plants. Pol J Environ Stud 18: 627-633.
  • Jafaar ZM, Litchfield LM, Ivanova MM, Radde BN, Al-Rayyan N, Klinge CM (2014) β-d-glucan inhibits endocrine-resistant breast cancer cell proliferation and alters gene expression. Int J Oncol 44: 1365-1375.
  • Kołodziejczyk L, Mazurkiewicz-Zapałowicz K, Janda K, Dzika E (2014) The effect of saprotrophic fungi on the development and hatching of Fasciola hepatica eggs. Folia Biol (Krakow) 62: 149-154.
  • Kvitek L, Panacek A, Prucek R, Soukupova J, Vanickova M, Kolar M, Zboril R (2011) Antibacterial activity and toxicity of silver - nanosilver versus ionic silver. Nanosafe2010: International Conference on Safe Production and Use of Nanomaterials. J Phys Conference Series 304: 012029.
  • Lai X, Zhang H, Liu R, Liu C (2015) Potential for aflatoxin B1 and B2 production by Aspergillus flavus strains isolated from rice samples. Saudi J Biol Sci 22: 176-180.
  • Lee J, Kim K-J, Sung WS, Kim JG, Lee DG (2010) The silver nanoparticle (nano-Ag): a new model for antifungal agents. In Silver nanoparticles. Perez Pozo D ed, pp 295-308. InTech.
  • Magnuson JK, Lasure LL (2004) Organic Acid Production by Filamentous Fungi. In Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine. Lange J, Lange L eds. Lange, Kluwer Academic / Plenum Publishers. p. 307-340.
  • Marino A, Nostro A, Fiorentino C (2009) Ochratoxin A production by Aspergillus westerdijkiae in orange fruit and juice. Int J Food Microbiol 132: 185-189.
  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16: 2346-2353.
  • Muñoz K, Vega M, Rios G, Geisen R, Degen GH (2011) Mycotoxin production by different ochratoxigenic Aspergillus and Penicillium species on coffee- and wheat-based media. Mycotoxin Res 27: 239-247.
  • Nasrollahi A, Pourshamsian K, Mansourkiaee P (2011) Antifungal activity of silver nanoparticles on some of fungi. Int J Nano Dimens 1: 233-239.
  • Nelson PE, Plattner RD, Shackelford DD, Desjardins AE (1992) Fumonisin B1 Production by Fusarium Species Other Than F. moniliforme in Section Liseola and by Some Related Species. Appl Environ Microbiol 58: 984-989.
  • Nowak A, Piotrowska M (2012) Biochemical activities of Brochothrix thermosphacta. Meat Science 90: 410-413.
  • Papamanoli E, Tzanetakis N, Litopoulou-Tzanetaki E, Kotzekidou P (2003) Characterization of lactic acid bacteria isolated from a Greek, dry-fermented sausage in respect of their technological and probiotic properties. Meat Science 65: 859-867.
  • Pinto RJ, Almeida A, Fernandes SC, Freire CS, Silvestre AJ, Neto CP, Trindade T (2013) Antifungal activity of transparent nanocomposite thin films of pullulan and silver against Aspergillus niger. Colloids Surf B Biointerfaces 103: 143-148.
  • Ramirez ML, Chulze S, Magan N (2004) Impact of environmental factors and fungicides on growth and deoxinivalenol production by Fusarium graminearum isolates from Argentinian wheat. Crop Protection 23: 117-125.
  • Ravi Babu G, Guru Prasad M, Prasad TNVKV (2011) Isolation and Quantification of Aflatoxin from Aspergillus flavus Infected Rice. Int J Pure Appl Sci Technol 5: 16-24.
  • Rheeder JP, Marasas WF, Vismer HF (2002) Production of Fumonisin Analogs by Fusarium Species. Appl Environ Microbiol 68: 2101-2105.
  • Ruijter GJ, van de Vondervoort PJ, Visser J (1999) Oxalic acid production by Aspergillus niger: an oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese. Microbiology 145: 2569-2576.
  • Scervino JM, Papinutti VL, Godoy MS, Rodriguez MA, Della Monica I, Recchi M, Pettinari MJ, Godeas AM (2011) Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production. J Appl Microbiol 110: 1215-1223
  • Soares C, Calado T, Venâncio A (2013) Mycotoxin production by Aspergillus niger aggregate strains isolated from harvested maize in three Portuguese regions. Rev Iberoam Micol 30: 9-13.
  • Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram negative bacteria. J Colloid Interface Sci 275: 177-182.
  • Susca A, Proctor RH, Mule G, Stea G, Ritieni A, Logrieco A, Moretti A (2010) Correlation of Mycotoxin Fumonisin B2 Production and Presence of the Fumonisin Biosynthetic Gene fum8 in Aspergillus niger from Grape. J Agric Food Chem 58: 9266-9272.
  • Tolaymat T, El Badawy A, Genaidy A, Scheckel K, Luxton T, Suidan M (2010) An evidence-based environmental perspective of manufactured silver nanoparticle in syntheses and applications: A systematic review and critical appraisal of peer reviewed scientific papers. Sci Total Environ 408: 999-1006.
  • West TP (2011) Malic acid production from thin stillage by Aspergillus species. Biotechnol Lett 33: 2463-2467.
  • Wiley B, Sun Y, Mayers B, Xia Y (2005) Shape-controlled synthesis of metal nanostructures: The case of silver. Chem Eur J 11: 454-463.
  • Zhang M, Chiu LM, Cheung PC, Ooi VE (2006) Growth-inhibitory effects of a ß-glucan from the mycelium of Poria cocos on human breast carcinoma MCF-7 cells: Cell-cycle arrest and apoptosis induction. Oncol Rep 15: 637-643.
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