Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate

• Authors: Anna Brillowska-Dąbrowska , Martyna Mroczyńska , Urszula Nawrot , Katarzyna Włodarczyk , Ewelina Kurzyk
• Languages of publication: EN

Abstracts

EN

Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries, however there has been no report from Poland. Here, we describe for the first time, an examination of cyp51A and cyp51B expression level of 11 clinical A. fumigatus strains isolated during 2007-2014 period from the collection of Medical University in Wrocław. Their susceptibility to itraconazole, voriconazole and posaconazole has been examined. The MIC values of triazoles for one of the examined isolates were respectively: > 8 mg/L for itraconazole, 2 mg/L for voriconazole and 0.5 mg/L for posaconazole. The cyp51A gene with its promoter region of all isolates was sequenced. It was found that the resistant isolate harbors the TR34/L98H mutation in the cyp51A gene and when cultured on media supplemented with voriconazole exhibits overexpression of both, cyp51A and cyp51B genes. The level of cyp51A gene expression was about 50 times higher than cyp51B.

Keywords

EN

Aspergillus fumigatus; azole resistance; cyp51A; cyp51B

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 837-839

Dates

published

2015

received

2015-07-30

revised

2015-09-21

accepted

2015-10-02

(unknown)

2015-12-04

Contributors

author

Anna Brillowska-Dąbrowska

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

author

Martyna Mroczyńska

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

author

Urszula Nawrot

• Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wrocław Medical University, Wrocław, Poland

author

Katarzyna Włodarczyk

• Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wrocław Medical University, Wrocław, Poland

author

Ewelina Kurzyk

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2015_1143