Application of the BIOLOG system for characterization of Serratia marcescens ss marcescens isolated from onsite wastewater technology (OSWT)

• Authors: Joanna Chojniak , Łukasz Jałowiecki , Elmar Dorgeloh , Berta Hegedusova , Helene Ejhed , Jörgen Magnér , Grażyna Płaza
• Languages of publication: EN

Abstracts

EN

The scope of this study was to apply the Biolog system to identify and characterize a Serratia strain isolated from the surface of black plastic pieces which constitute the fluidized bed filter (onsite wastewater technology, OSWT). The preliminary isolation of the strain was done in the medium with tetracycline at a 16 mg/l concentration. To characterize the isolated strain, the following Biolog methods were applied: (1) EcoPlates microplates for evaluation of physiological profiling, (2) GEN III OmniLog® ID System for identification of the isolate, and (3) phenotypic microarrays (PM) technology for evaluation of sensitivity to antibiotics (PM11 and PM12). Results were recorded using the original OmniLog® software. The Serratia strain was identified as Serratia marcescens ss marcescens with similarity index 0.569. The same identification was obtained by the 16S rDNA analysis. PM analysis showed an enhancement of phenotype (resistance or growth) of this strain to 35 antibiotics. The loss of phenotype (sensitivity or non-growth) was observed only for 5 antibiotics: lomefloxacin (0.4 µg/ml), enoxacin (0.9 µg/ml), nalidixic acid (18.0 µg/ml), paromomycin (25.0 µg/ml) and novobiocin (1100 µg/ml). This study acknowledges that the methods proposed by the Biolog system allow correct and complete identification and characterization of the microbes isolated from different environments. Phenotypic microarrays could be successfully used as a new tool for identification of the multi-antibiotic resistance of bacteria and for determination of the minimal inhibition concentrations (MIC).

Keywords

EN

onsite wastewater treatment; Biolog system; Serratia spp.; antibiotic resistance

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 799-805

Dates

published

2015

received

2015-07-29

revised

2015-10-21

accepted

2015-10-30

(unknown)

2015-12-03

Contributors

author

Joanna Chojniak

• Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland

author

Łukasz Jałowiecki

• Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland

author

Elmar Dorgeloh

• Development and Assessment Institute in Waste Water Technology at RWTH Aachen University, Germany

author

Berta Hegedusova

• Development and Assessment Institute in Waste Water Technology at RWTH Aachen University, Germany

author

Helene Ejhed

• Natural Resources & Environmental Effects, IVL Swedish Environmental Research Institute, Sweden

author

Jörgen Magnér

• Natural Resources & Environmental Effects, IVL Swedish Environmental Research Institute, Sweden

author

Grażyna Płaza

• Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland

References

• Al-Mutairi NZ (2009) Variable distributional characteristics of substrate utilization patterns in activated sludge plants in Kuwait. Bioresour Technol 100: 1524-1532.
• Biondi EG, Tatti E, Comparini D, Giuntini E, Mocali S, Giovannetti L, Bazzicalupo M, Mengoni A, Viti C (2009) Metabolic capacity of Sinorhizobium (Ensifer) meliloti strains as determined by phenoptype microarray analysis. Appl Environ Microbiol 75: 5396-5404.
• Bochner BR, Gadzinski P, Panomitros E (2001) Phenotype MicroArrays for high-throughput phenotypic testing and assay of gene function. Genome Res 11: 1246-1255.
• Bochner BR (2008) Global phenotypic characterization of bacteria. FEMS Microbiol Rev 33: 191-205.
• Bucher AE, Lanyon LE (2005) Evaluation soil management with microbial community-level physiological profiles. Appl Soil Ecol 29: 59-71.
• Casullo de Araujo HW, Fukushima K, Campos Takaki GM (2010) Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules 15: 6931-6940.
• Chen F, Zheng H, Zhang K, Ouyang Z, Lan J, Li H, Shi Q (2013) Changes in soil microbial community structure and metabolic activity following conversion from native Pinus massoniana plantations to exotic Eucalyptus plantations. For Ecol Manage 291: 65-72.
• Decorosi F, Santopolo L, Mora D, Viti C, Giovannetti L (2011) The improvement of a phenotype microarray protocol for the chemical sensitivity analysis of Streptococcus thermopilus. J Microbiol Methods 86: 258-261.
• Drancourt M, Berger P, Raoult D (2004) Systematic 16S rRNA gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans. J Clin Microbiol 42: 2197-2202.
• Garcia SN, Clubbs RL, Stanley JK, Scheffe B, Yelderman JC Jr, Brooks BW (2013) Comparative analysis of effluent water quality from a municipal treatment plant and two on-site wastewater treatment systems. Chemosphere 92: 38-44.
• Garland J, Mills A (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl Environ Microbiol 57: 2351-2359.
• Gomez E, Garland J, Conti M (2006) Reproducibility in the response of soil bacterial community-level physiological profiles from a land use intensification gradient. Appl Soil Ecol 26: 21-30.
• Grimont F, Grimont PAD (1992) The genus Serratia. In The Prokayotes: a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Balows A, Truper GG, Dworkin M, Harder W, Schleifer KH, eds, pp 2822-2848. Springer, New York, Berlin.
• Gryta A, Frąc M, Oszust K (2014) The application of the Biolog EcoPlates approach in ecotoxicological evaluation of dairy sewage sludge. Appl Biochem Biotechnol 174: 1434-1443.
• Hejazi A, Falkiner F R (1997) Serratia marcescens. J Med Microbiol 46: 903-912.
• Line JE, Hiet KL, Guard-Bouldin J, Seal BS (2010) Differential carbon source utilization by Campylobacter jejuni 11168 in response to growth temperature variation. J Microbiol Methods 80: 198-202.
• Kenarova A, Radeva G, Traykov I, Boteva S (2014) Community level physiological profiles of bacteria communities inhabiting uranium mining impacted sites. Ecotoxicol Environ Safety 100: 226-232.
• Kolbert CP, Persing DH (1999) Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 2: 299-305.
• Kong X, Wang C, Ji M (2013) Analysis of microbial metabolic characteristics in mesophilic and thermophilic biofilters using Biolog plate technique. Chem Eng J 230: 415-421.
• Lucas A, Manna AC (2013) Phenotypic characterization of sarR mutant in Staphylococcus aureus. Microb Pathog 57: 52-61.
• Mazur A, Stasiak G, Wielbo J, Koper P, Kubik-Komar A, Skorupska A (2013) Phenotype profiling of Rhizobium leguminosarum bv. Trifolii clover nodule isolates reveal their both versatileand specialized metabolic capabilities. Arch Microbiol 195: 255-267.
• Mesak LR, Davies J (2009) Phenotypic changes in ciprofloxacin-resistant Staphylococcus aureus. Res Microbiol 160: 785-791.
• Park M, Rafii F (2014) Global phenotypic characterization of effects of fluoroquinolone resistance selection on the metabolic activities and drug susceptibilities of Clostridium perfingens strains. Int J Microbiol 2014: 456979.
• Paul D, Pandey G, Meier C, van der Meer JR, Jain RK (2006) Bacterial community structure of a pesticide-contaminated site and assessment of changes induced in community structure during bioremediation. FEMS Microbiol Ecol 57: 116-127.
• Preston-Mafham J, Boddy L, Randerson PF (2002) Analysis of microbial community functional diversity using sole-carbon-source utilization profiles - a critique. FEMS Microbiol Ecol 42: 1-14.
• Sala MM, Arrieta JM, Boras JA, Duarte CM, Vaque D (2010) The impact of ice melting on bacterioplankton in the Arctic Ocean. Polar Biol 33: 1683-1694.
• Sleigh JD (1983) Antibiotic resistance in Serratia marcescens. BMJ 287: 1651-1653.
• Stefanowicz A (2006) The Biolog plates technique as a tool in ecological studies of microbial communities. Polish J Environ Stud 15: 669-676.
• Stock I, Grueger T, Wiedemann B (2003) Natural antibiotic susceptibility of strains of Serratia marcescens and the S. liquefaciens complex: S. liquefaciens sensu stricto, S. proteamaculans and S. grimesii. Int J Antimicrob Agents 22: 35-47.
• Sundaramoorthy N, Yogesh P, Dhandapani R (2009) Production of prodigiosin from Serratia marcescens isolated from soil. Indian J Sci Technol 2: 32-34.
• Traub WH (2000) Antibiotic susceptibility of Serratia marcescens and Serratia liquefaciens. Chemotherapy 46: 315-321.
• Venil CK, Velmurugan P, Lakshmanaperumalsamy P (2009) Genomic environment of cueR and copA genes for prodigiosin biosynthesis by Serratia marcescens SB08. Romanian Biotechnol Lett 14: 4812-4819.
• Ventura M, Zink R (2002) Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis. FEMS Microbiol Lett 217: 141-154.
• Weber K, Legge R (2009) One-dimensional metric for tracking bacterial community divergence using sole carbon source utilization patterns. J Microbiol Methods 79: 55-61.
• Zhang J, Biswas I (2009) A phenotypic microarray analysis of a Streptococcus mutans liaS mutant. Microbiology 155: 61-68.
• Zhang CB, Wang J, Liu WL, Zhu SX, Ge HL, Chang SX, Chang J, Ge Y (2010) Effects of plant diversity on microbial biomass and community metabolic profiles in a full-scale constructed wetland. Ecol Eng 36: 62-68.

Identifiers

DOI

10.18388/abp.2015_1138