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2015 | 62 | 4 | 785-790
Article title

Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.

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Alicyclobacillus sp. are acidothermophilic bacteria frequently contaminating fruit based products (juices and juice concentrates). These sporulating bacteria are able to survive at elevated temperatures and highly acidic environments which causes difficulties in their removal from industrial environments. Although numerous literature data examine Alicyclobacillus sp. presence in fruit based products and methods of their elimination, there is still a limited knowledge on ability of these bacteria to adhere to abiotic surfaces. Therefore, the objective of this study was to determine Alicyclobacillus sp. cells' hydrophobicity and capability of biofilm formation on a glass surface. The degree of cells hydrophobicity, according to Microbial Adhesion to Hydrocarbon (MATH) and Salt Aggregation Test (SAT), was investigated for eleven environmental isolates from natural Polish habitats, identified as Alicyclobacillus sp., and a Alicyclobacillus acidoterrestris DSM 3922 reference strain. The dynamics of biofilm formation within 3-day incubation on a glass surface was evaluated and quantified by a plate count method both, for cultures with and without agitation. All of the bacterial strains tested expressed ability to colonize a glass surface and four environmental isolates were classified as fast-adherent strains. The mature biofilm structures were predominantly formed after 48 hours of incubation. Dynamic culturing conditions were observed to accelerate the biofilm formation. The majority of strains expressed a moderate hydrophobicity level both, in SAT (41.7%) and MATH-PBS (75.0%), as well as MATH-PUM (91.7%) tests. However, no correlation between hydrophobicity and cell adherence to a glass slide surface was observed.
Physical description
  • Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland
  • Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland
  • Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland
  • Abee T, Kovács T, Kuipers OP, Veen S (2011) Biofilm formation and dispersal in Gram-positive bacteria. Curr Opin Biotechnol 22: 172-179.
  • AIJN European Fruit Juice Association (2014) Liquid fruit market report.
  • Basson A, Flemming LA, Chenia HY (2007) Evaluation of adherence, hydrophobicity, aggregation and biofilm development of Flavobacterium johnsoniae-like isolates. Microb Ecol 55: 1-14.
  • Cerca N, Pier GB, Vilanova M, Oliveira R, Azeredo J (2005) Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis. Res Microbiol 156: 506-514.
  • Cerny G, Hennlich W, Poralla K (1984) Fruchtsaftverderb durch Bacillen: isolierung und charakterisierung des verderbserregers. Z Lebensm Unters Forsch 179: 224-227.
  • Chang S, Kang DH (2004) Alicyclobacillus spp. in the fruit juice industry: history, characteristics, and current isolation/detection procedures. Crit Rev Microbiol 30: 55-74.
  • Di Bonaventura G, Piccolomini R, Paludi D, D'Orio V, Vergara A, Conter M, Ianieri A (2007) Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity. J Appl Microbiol 104: 1552-1561.
  • dos Anjos MM, Ruiz SP, Nakamura CV, de Abreu Filho BA (2013) Resistance of Alicyclobacillus acidoterrestris spores and biofilm to industrial sanitizers. J Food Prot 76: 1408-1413.
  • Garret TR, Bhakoo M, Zhang Z (2008) Bacterial adhesion and biofilms on surfaces. Prog Nat Sci 18: 1049-1056.
  • Groenewald WH, Gouws PA, Witthuhn RC (2008) Isolation and identification of species of Alicyclobacillus from orchard soil in the Western Cape, South Africa. Extremophiles 12: 159-163.
  • Hippchen B, Roll A, Poralla K (1981) Occurrence in soil of thermo-acidophilic bacilli possessing ω-cyclohexane fatty acids and hopanoids. Arch Microbiol 129: 53-55.
  • Kadam TA, Rupa L, Balhal DK, Totewad ND, Gyananath G (2009) Determination of the degree of hydrophobicity - a technique to assess bacterial colonization on the leaf surface and root region of lotus plant. Asian J Exp Sci 23: 135-139.
  • Kolwzan B (2011) Analysis of biofilms - their formation and functioning. Ochrona Srodowiska 33: 3-14.
  • Ljungh A, Wadström T (1982) Salt aggregation test for measuring cell surface hydrophobicity of urinary Escherichia coli. Eur J Clin Microbiol 1: 388-396.
  • Marques SC, Rezende JGO, Alves LAF, Silva BA, Alves E, Abreu LR, Piccoli RH (2007) Formation of biofilm by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected sanitizers. Braz J Microbiol 38: 538-543.
  • Myszka K, Czaczyk K (2011) Bacterial biofilms on food contact surfaces - a review. Pol J Food Nutr Sci 61: 173-180.
  • Nwanyanwu CE, Abu GO (2013) Influence of growth media on hydrophobicity of phenol-utilizing bacteria found in petroleum refinery effluent. Int Res J Biological Sci 2: 6-10.
  • Olszewska MA (2013) Microscopic findings for the study of biofilms in food environments. Acta Biochim Pol 60: 531-537.
  • Ortega MP, Hagiwara T, Watanabe H, Sakiyama T (2008) Factors affecting adhesion of Staphylococcus epidermidis to stainless steel surface. Jap J Food Eng 9: 251-259.
  • Podolak R, Elliott PH, Taylor BJ, Khurana A, Black DG (2009) Destruction of Alicyclobacillus acidoterrestris spores in apple juice on stainless steel surfaces by chemical disinfectants J Food Prot 72: 510-514.
  • Qiao G, Li H Xu, DH, Park SI (2012) Modified a colony forming unit microbial adherence to hydrocarbons assay and evaluated cell surface hydrophobicity and biofilm production of Vibrio scophthalmi. J Bacteriol Parasitol 3: 130.
  • Rosenberg M (1984) Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity. FEMS Microbiol Lett 22: 289-295.
  • Shemesh M, Pasvolsky R, Zakin V (2014) External pH is a cue for the behavioral switch that determines surface motility and biofilm formation of Alicyclobacillus acidoterrestris. J Food Prot 77: 1418-1423.
  • Shi X, Zhu X (2009) Biofilm formation and food safety in food industries. Trends Food Sci Technol 20: 407-413.
  • Stewart PS (2002) Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol 292: 107-113.
  • Smit Y, Cameron M, Venter P, Witthuhn RC (2011) Alicyclobacillus spoilage and isolation - a review. Food Microbiol 28: 331-349.
  • Sokolowska B (2014) Alicyclobacillus - termofilne kwasolubne bakterie przetrwalnikujące - charakterystyka i wystepowanie. ZNTJ 4: 5-17.
  • Splittstoesser DF, Churey JJ, Lee CY (1994) Growth characteristics of aciduric sporeforming bacilli isolated from fruit juices. J Food Prot 57: 1080-1083.
  • Srey S, Jahid IK, Ha SD (2013) Biofilm formation in food industries: a food safety concern. Food Control 31: 572-585.
  • Tianli Y, Jiangbo Z, Yahong Y (2014) Spoilage by Alicyclobacillus bacteria in juice and beverage products: chemical, physical, and combined control methods. Compr Rev Food Sci F 13: 771-797.
  • Van Loosdrecht MCM, Lykema J, Norde W, Scraa G, Zehnder AJB (1987) The role of bacterial cell wall hydrophobicity in adhesion. Appl Environ Microbiol 53: 1893-1897.
  • Walls I, Chuyate R (1998) Alicyclobacillus - historical perspective and preliminary characterization study. Dairy Food Environ Sanit 18: 499-503.
  • Wisotzkey JD, Jurtshuk P, Fox GE, Deinhard G, Poralla K (1992) Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42: 263-269.
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