The phenotypic and genomic diversity of Aspergillus strains producing glucose dehydrogenase

• Authors: Beata Rola , Anna Pawlik , Magdalena Frąc , Wanda Małek , Zdzisław Targoński , Jerzy Rogalski , Grzegorz Janusz
• Languages of publication: EN

Abstracts

EN

Twelve Aspergillus sp. strains producing glucose dehydrogenase were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was investigated. Moreover, partial gdh gene sequences were determined and aligned. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of twelve Aspergillus isolates. Using one PstI restriction endonuclease and five selective primers in an AFLP assay, 556 DNA fragments were generated, including 532 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished twelve Aspergilli fungi. The AFLP-based dendrogram generated by the UPGMA method grouped all the Aspergillus fungi studied into two major clusters. All the Aspergillus strains were also characterized using Biolog FF MicroPlates to obtain data on C-substrate utilization and mitochondrial activity. The ability to decompose various substrates differed among the analyzed strains up to three folds. All of the studied strains mainly decomposed carbohydrates.

Keywords

EN

Aspergillus; glucose dehydrogenase; identification; metabolic diversity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 747-755

Dates

published

2015

received

2015-07-22

revised

2015-10-02

accepted

2015-10-30

(unknown)

2015-12-01

Contributors

author

Beata Rola

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Anna Pawlik

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Magdalena Frąc

• Institute of Agrophysics Polish Academy of Sciences, Lublin, Poland

author

Wanda Małek

• Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland

author

Zdzisław Targoński

• Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences, Lublin, Poland

author

Jerzy Rogalski

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Grzegorz Janusz

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

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Identifiers

DOI

10.18388/abp.2015_1125