The effects of nickel(II) complexes with imidazole derivatives on pyocyanin and pyoverdine production by Pseudomonas aeruginosa strains isolated from cystic fibrosis

• Authors: Katarzyna Gałczyńska , Krystyna Kurdziel , Wioletta Adamus-Białek , Sławomir Wąsik , Karol Szary , Marcin Drabik , Aneta Węgierek-Ciuk , Anna Lankoff , Michał Arabski
• Languages of publication: EN

Abstracts

EN

Pseudomonas aeruginosa infection is problematic in patients with cystic fibrosis (CF). P. aeruginosa secretes a diversity of pigments, such as pyocyanin and pyoverdine. The aim of this study was to evaluate the effects of complexes of nickel(II) ([Ni(iaa)2(H2O)2]·H2O (iaa = imidazole-4-acetate anion), [Ni(1-allim)6](NO3)2 (1-allim = 1-allylimidazole) and NiCl2 on pyocyanin and pyoverdine production by 23 strains of P. aeruginosa isolated from cystic fibrosis under growth conditions specific for the CF respiratory system. The antibacterial effects and biophysical properties of the tested substances were measured by spectrofluorometric techniques, as well as by laser interferometry, confocal and atomic force microscopy. The cytotoxic properties of all compounds were measured by Annexin/IP assay against A549 cells. All tested compounds have no effect on pyocyanin production and decrease the pyoverdine secretion in about 40% of tested P. aeruginosa strains at non-cytotoxic range of concentrations. Imidazole-4-acetate anion and 1-allylimidazole have good diffusion properties in the mature P. aeruginosa PAO1 biofilm. In conclusion, the tested nickel(II) complexes do not have clinical implications in P. aeruginosa eradication in cystic fibrosis. The diffusion properties of 1-allylimidazole and imidazole-4-acetate and their lack of effect on A549 cells suggest that they might be considered for chemical synthesis with other transition metals.

Keywords

EN

Pseudomonas sp.; cystic fibrosis; nickel complexes; pyocyanin; pyoverdine

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 739-745

Dates

published

2015

received

2015-07-22

revised

2015-10-26

accepted

2015-11-30

(unknown)

2015-12-08

Contributors

author

Katarzyna Gałczyńska

• Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland

author

Krystyna Kurdziel

• Institute of Chemistry, Jan Kochanowski University, Kielce, Poland

author

Wioletta Adamus-Białek

• Department of Environment Protection and Modelling, Jan Kochanowski University, Kielce, Poland

author

Sławomir Wąsik

• Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland

author

Karol Szary

• Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland

author

Marcin Drabik

• Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland

author

Aneta Węgierek-Ciuk

• Department of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland

author

Anna Lankoff

• Department of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland
• Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, Poland

author

Michał Arabski

• Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland

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Identifiers

DOI

10.18388/abp.2015_1124