Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces

• Authors: Julia Zabielska , Alina Kunicka-Styczyńska , Katarzyna Rajkowska , Agnieszka Tyfa
• Languages of publication: EN

Abstracts

EN

Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (107-108 CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm2 after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm2. This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.

Keywords

EN

Pseudomonas aeruginosa; biofilm; environmental strains; polymer surfaces

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 733-737

Dates

published

2015

received

2015-07-30

revised

2015-10-19

accepted

2015-10-22

(unknown)

2015-11-30

Contributors

author

Julia Zabielska

• Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

author

Alina Kunicka-Styczyńska

• Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

author

Katarzyna Rajkowska

• Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

author

Agnieszka Tyfa

• Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

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Identifiers

DOI

10.18388/abp.2015_1121