Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 4 | 725-732
Article title

Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria

Title variants
Languages of publication
The aim of this study was to investigate the antimicrobial effect of lipopeptide biosurfactants from surfactin, iturin and fengycin families, synthesised by the Bacillus subtilis I'1a strain, on uropathogenic bacteria, including the effects on planktonic growth, processes of biofilm formation and dislodging. Antimicrobial activity was tested against 32 uropathogenic strains belonging to 12 different species of Gram-negative and Gram-positive bacteria. The sensitivity of 25 tested bacterial strains to the B. subtilis I'1a filtrate was confirmed by an agar diffusion assay. None of the strains seemed to be sensitive to pure surfactin at concentrations ranging from 0.1 mg × ml-1 to 0.4 mg ml-1. After the treatment of uropathogens with B. subtilis lipopeptides, the metabolic activity of planktonic cells was inhibited by 88.05±3.96% in the case of 21 studied uropathogens, the process of biofilm formation was reduced by 88.15±4.77% in the case of 24 uropathogens and mature biofilms of 18 strains were dislodged by about 81.20±4.72%. Ten strains of uropathogenic bacteria were selected to study the antimicrobial activity of surfactin (concentrations 0.1, 0.2 and 0.4 mg × ml-1). Surfactin had no influence on the metabolic activity of planktonic forms of uropathogens, however, biofilms of 5 tested strains were reduced by 64.77±9.05% in the presence of this biosurfactant at the concentration 0.1 mg × ml-1. The negative effect of the compound on the biofilm formation process was observed at all concentrations used. The above-described results were fully confirmed by CLSM. It could suggest that synergistic application of biosurfactants could be efficient in uropathogen eradication.
Physical description
  • Department of Immunobiology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Immunobiology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Immunobiology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Institute for Ecology of Industrial Areas, Department of Environmental Microbiology, Katowice, Poland
  • Department of Immunobiology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Araujo LV, Lins UC, Santa Anna LMM, Nitschke M, Freire DMG (2011) Rhamnolipid and surfactin inhibit Listeria monocytogenes adhesion. Food Res int 44: 481-488.
  • Bacon CW, Hinton DM, Mitchell TR, Snook ME, Olubajo B (2012) Characterization of endophytic strains of Bacillus mojavensis and their production of surfactin isomers. Biol Control 62: 1-9.
  • Berry CJ, Story S, Altman DJ, Upchurch R, Whitman W, Singleton D, Płaza G, Brigmon RL (2006) Biological treatment of petroleum and radiological contaminated soil. In Innovative approaches for the remediation of subsurface-contaminated hazardous waste sites. Bridging Flask and Field Scales. Clayton C & Lindner A, eds, pp 87-104. Oxford University Press, Oxford.
  • Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A (2015) The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity. Antonie Van Leeuwenhoek 108: 343-353.
  • Chen H, Wang L, Su CX, Gong GH, Wang P, Yu ZL (2008) Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Lett Appl Microbiol 47: 180-186.
  • Chen L, Wen Y (2011) The role of bacterial biofilm in persistent infections and control strategies. Int J Oral Sci 3: 66-73.
  • Compaoré CS, Nielsen DS, Ouoba LI, Berner TS, Nielsen KF, Sawadogo-Lingani H, Diawara B, Ouédraogo GA, Jakobsen M, Thorsen L (2013) Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from Bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment. Int J Food Microbiol 162: 297-307.
  • Das P, Mukherjee S, Sen R (2009) Substrate dependent production of extracellular biosurfactant by a marine bacterium. Bioresour Technol 100: 1015-1019.
  • Deleu M, Lorent J, Lins L, Brasseur R, Braun N, El Kirat K, Nylander T, Dufrêne YF, Mingeot-Leclercq MP (2013) Effects of surfactin on membrane models displaying lipid phase separation. Biochim Biophys Acta 1828: 801-815.
  • Donlan RM (2002) Biofilms: microbial life on surfaces. Emerg Infect Dis 8: 881-890.
  • Hamley IW (2015) Lipopeptides: from self-assembly to bioactivity. Chem Commun (Camb) 51: 8574-8583.
  • Janek T, Łukaszewicz M, Krasowska A (2012) Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5. BMC Microbiol 12: 24.
  • Kim PI, Ryu J, Kim YH, Chi YT (2010) Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol 20: 138-145.
  • Li Y, Yang S, Mu B (2010) The surfactin and lichenysin isoforms produced by Bacillus licheniformis HSN 221. Anal Lett 43: 929-940.
  • Loiseau C, Schlusselhuber M, Bigot R, Bertaux J, Berjeaud JM, Verdon J (2015) Surfactin from Bacillus subtilis displays an unexpected anti-Legionella activity. Appl Microbiol Biotechnol 99: 5083-5093.
  • Meena KR, Kanwar SS (2015) Lipopeptides as the antifungal and antibacterial agents: applications in food safety and therapeutics. Biomed Res Int 2015: 473050.
  • Mireles JR 2nd, Toguchi A, Harshey RM (2001) Salmonella enterica serovar typhimurium swarming mutants with altered biofilm-forming abilities: surfactin inhibits biofilm formation. J Bacteriol 183: 5848-5854.
  • Mnif I, Ghribi D (2015) Review lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications. Biopolymers 104: 129-147.
  • Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol 16: 115-125.
  • Ostroumova OS, Malev VV, Ilin MG, Schagina LV (2010) Surfactin activity depends on the membrane dipole potential. Langmuir 26: 15092-15097.
  • Paraszkiewicz K, Długoński J (2007) Remediation of heavy metal-contaminated soil by microbial surfactants. Biotechnologia 2: 81-94 (in Polish).
  • Pecci Y, Rivardo F, Martinotti MG, Allegrone G (2010) LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain. J Mass Spectrom 45: 772-778.
  • Płaza G, Chojniak J, Rudnicka K, Paraszkiewicz K, Bernat P (2015) Detection of biosurfactants in Bacillus species: genes and products identification. J Appl Microbiol 119.
  • Płaza G, Gawior K, Jangid K, Wilk K (2010) Characterization of surface active properties of Bacillus strains growing in brewery effluent. In Environmental Engineering III. Pawłowski L, Dudzińska MR,. Pawłowski A, eds, pp 221-226. Taylor and Francis Group, London.
  • Płaza G, Pacwa-Płociniczak M, Piotrowska-Seget Z, Jangid K, Wilk K (2011) Agroindustrial wastes as unconventional substrates for growing of Bacillus strains and production of biosurfactants. Env Prot Eng 37: 65-71.
  • Płaza G, Zjawiony I, Banat I (2006) Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated and bioremediated soil. J Petrol Sci Eng 50: 71-77.
  • Rivardo F, Turner RJ, Allegrone G, Ceri H, Martinotti MG (2009) Anti-adhesion activity of two biosurfactants produced by Bacillus spp. prevents biofilm formation of human bacterial pathogens. Appl Microbiol Biotechnol 83: 541-553.
  • Sabate DC, Audisio MC (2013) Inhibitory activity of surfactin, produced by different Bacillus subtilis subsp. subtilis strains, against Listeria monocytogenes sensitive and bacteriocin-resistant strains. Microbiol Res 168: 125-129.
  • Singh AK, Rautela R, Cameotra SS (2014) Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2. Microb Cell Fact 13: 67.
  • Wang T, Liang Y, Wu M, Chen Z, Lin J, Yang L (2015) Natural products from Bacillus subtilis with antimicrobial properties. Chin J Chem Eng 23: 744-754.
  • Woźniak-Kosek A (2013) The phenomenon of biofilm - conditions of its formation and functioning in an environment. Mil Phar Med 6: 14-22.
  • Zezzi do Valle Gomez M, Nitschke M (2012) Evaluation of rhamnolipid and surfactin to reduce the adhesion and remove biofilms of individual and mixed cultures of food pathogenic bacteria. Food Control 25: 441-447.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.