PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 4 | 707-712
Article title

PCR and real-time PCR assays to detect fungi of Alternaria alternata species

Content
Title variants
Languages of publication
EN
Abstracts
EN
Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria infections may be indistinguishable from other fungal diseases. Therefore, a diagnostic result is often delayed or even not achieved at all. In this paper we present easy to perform and interpret PCR and real-time PCR assays enabling detection of A. alternata species. On the basis of alignment of β-tubulin gene sequences, A. alternata-specific primers were designed. DNA from fungal isolates, extracted in a two-step procedure, were used in PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The assays specificity was confirmed, since positive results were obtained for all A. alternata isolates, and no positive results were obtained neither for other molds, dermatophytes, yeast-like fungi, nor human DNA. The assays developed here enable fast and unambiguous identification of A. alternata pathogens.
Publisher

Year
Volume
62
Issue
4
Pages
707-712
Physical description
Dates
published
2015
received
2015-07-30
revised
2015-10-02
accepted
2015-10-07
(unknown)
2015-10-27
Contributors
  • Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland
  • Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland
  • Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland
  • Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland
References
  • Badenoch PR, Halliday CL, Ellis DH, Billing KJ, Mills RA (2006) Ulocladium atrum keratitis. J Clin Microbiol 44: 1190-1193.
  • Brás S, Sabino R, Laureano A, Simões H, Fernandes C, Marques-Pinto G, Cardoso J, Veríssimo C (2015) Cutaneous infection by different Alternaria species in a liver transplant recipient. Med Mycol Case Rep 8: 1-4.
  • Brillowska-Dąbrowska A, Nielsen SS, Nielsen HV, Arendrup MC (2010) Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory. Med Mycol 48: 828-831.
  • Bush RK, Prochnau JJ (2004) Alternaria-induced asthma. J Allergy Clin Immunol 113: 227-234.
  • Calabrò G, Nino M, Gallo L, Scalvenzi M (2008) Cutaneous alternariosis in a kidney transplantation recipient: report of a case. J Dermatolog Treat 19: 246-248.
  • Chowdhary A, Agarwal K, Randhawa HS, Kathuria S, Gaur SN, Najafzadeh MJ, Roy P, Arora N, Khanna G, Meis JF (2012) A rare case of allergic bronchopulmonary mycosis caused by Alternaria alternata. Med Mycol 50: 890-896.
  • de Hoog GS, Guarro J, Gené J, Figueras MJ (2000) Atlas of clinical fungi, 2nd edn. The Netherlands: Centraalbureau voor Schimmelcultures.
  • de Hoog GS, Horré R (2002) Molecular taxonomy of the Alternaria and Ulocladium species from humans and their identification in the routine laboratory. Mycoses 45: 259-276.
  • Durán MT, Del Pozo J, Yebra MT, Crespo MG, Paniagua MJ, Cabezón MA, Guarro J (2003) Cutaneous infection caused by Ulocladium chartarum in a heart transplant recipient: case report and review. Acta Derm Venereol 83: 218-221.
  • Gianni C, Cerri A, Crosti C (1997) Ungual phaeohyphomycosis caused by Alternaria alternata. Mycoses 40: 219-221.
  • Gomes J, Vilarinho C, Duarte Mda L, Brito C (2011) Cutaneous phaeohyphomycosis caused by Alternaria alternata unresponsive to itraconazole treatment. Case Rep Dermatol Med 2011: 385803.
  • Konidaris V, Mersinoglou A, Vyzantiadis TA, Papadopoulou D, Boboridis KG, Ekonomidis P (2013) Corneal Transplant Infection due to Alternaria alternata: A Case Report. Case Rep Ophthalmol Med 2013: 589620.
  • Kpodzo DS, Calderwood MS, Ruchelsman DE, Abramson JS, Piris A, Winograd JM, Kotton CN (2011) Primary subcutaneous Alternaria alternata infection of the hand in an immunocompromised host. Med Mycol 49: 543-547.
  • Kucan JO, Hall S (1985) Alternaria burn wound sepsis. J Burn Care Rehabil 6: 501-502.
  • Laich FS, Alcoba-Flórez J, Pérez-Roth E, Bahaya Y, Delgado JL, Méndez-Alvarez S (2008) Molecular characterization of Alternaria alternata causing ocular infection: detection of IGS-RFLP intraspecific polymorphism. Med Mycol 46: 615-619.
  • Lobritz RW, Roberts TH, Marraro RV, Carlton PK, Thorp DJ (1979) Granulomatous pulmonary disease secondary to Alternaria. JAMA 241: 596-597.
  • Lyke KE, Miller NS, Towne L, Merz WG (2001) A case of cutaneous ulcerative alternariosis: rare association with diabetes mellitus and unusual failure of itraconazole treatment. Clin Infect Dis 32: 1178-1187.
  • Pant H, Kette FE, Smith WB, Wormald PJ, Macardle PJ (2005) Fungal-specific humoral response in eosinophilic mucus chronic rhinosinusitis. Laryngoscope 115: 601-606.
  • Pastor FJ, Guarro J (2008) Alternaria infections: laboratory diagnosis and relevant clinical features. Clin Microbiol Infect 14: 734-746.
  • Pereiro M Jr, Pereiro Ferreirós MM, de Hoog GS, Toribio J (2004) Cutaneous infection caused by Alternaria in patients receiving tacrolimus. Med Mycol 42: 277-282.
  • Pesic Z, Otasevic S, Mihailovic D, Petrovic S, Arsic-Arsenijevic V, Stojanov D, Petrovic M (2015) Alternaria-associated fungus ball of orbit nose and paranasal sinuses: case report of a rare clinical entity. Mycopathologia 180: 99-103.
  • Pulimood TB, Corden JM, Bryden C, Sharples L, Nasser SM (2007) Epidemic asthma and the role of the fungal mold Alternaria alternata. J Allergy Clin Immunol 120: 610-617.
  • Revankar SG, Patterson JE, Sutton DA, Pullen R, Rinaldi MG (2002) Disseminated phaeohyphomycosis: review of an emerging mycosis. Clin Infect Dis 34: 467-476.
  • Romano C, Paccagnini E, Difonzo EM (2001) Onychomycosis caused by Alternaria spp. in Tuscany, Italy from 1985 to 1999. Mycoses 44: 73-76.
  • Romano C, Maritati E, Paccagnini E, Massai L (2004) Onychomycosis due to Ulocladium botrytis. Mycoses 47: 346-348.
  • Salido-Vallejo R, Linares-Sicilia MJ, Garnacho-Saucedo G, Sánchez-Frías M, Solís-Cuesta F, Gené J, Moreno-Giménez JC (2014) Subcutaneous phaeohyphomycosis due to Alternaria infectoria in a renal transplant patient: surgical treatment with no long-term relapse. Rev Iberoam Micol 31: 149-151.
  • Shugar MA, Montgomery WW, Hyslop NE (1981) Alternaria sinusitis. Ann Otol Rhinol Laryngol 90: 251-254.
  • Silveira CJ, Amaral J, Gorayeb RP, Cabral J, Pacheco T (2013) Fungal meningoencephalitis caused by Alternaria: a clinical case. Clin Drug Investig 33 (Suppl 1): S27-S31.
  • Stark PC, Celedon JC, Chew GL, Ryan LM, Burge HA, Muilenberg ML, Gold DR (2005) Fungal levels in the home and allergic rhinitis by 5 years of age. Environ Health Perspect 113: 1405-1409.
  • White TJ, Bruns T, S. Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds, pp 315-322. Academic Press, Inc., New York.
  • Yang S, Rothman RE (2004) PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings. Lancet Infect Dis 4: 337-348.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p707kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.