Dietary resistant dextrins positively modulate fecal and cecal microbiota composition in young rats

• Authors: Katarzyna Śliżewska , Zdzisława Libudzisz , Renata Barczyńska , Janusz Kapuśniak , Zenon Zduńczyk , Jerzy Juśkiewicz
• Languages of publication: EN

Abstracts

EN

The objective of the present study was to demonstrate the effect of dietary resistant dextrins, as potential prebiotics, on the intestinal microflora of young rats. Enzyme-resistant dextrin, prepared by heating of potato starch in the presence of hydrochloric (0.1% dsb) and tartaric (40% dsb) acid at 130ºC for 2 h (CA-dextrin). The experiment was performed on 24 Wistar male rats at 3-wk of age, divided by analogues in three experimental groups (control, starch and dextrin). Analyses determined the overall bacterial counts and the counts of Lactobacillus, Bifidobacterium, Bacteroides and Clostridium strains within the feces and cecal contents of rats using fluorescence in situ hybridization method. CA-dextrin had no effect on primary growth indicators (body weight, body weight gain, dietary consumption) or the mass of the small intestine and the cecum, but dextrins caused a reduction in pH and the concentration of ammonia within the cecal contents. That supplementation of diet with resistant dextrins had a positive effect on composition of intestinal microflora in rats. It increased the counts of Bifidobacterium and Lactobacillus strains both in the feces and in the cecum. Moreover, it reduced the counts of Clostridium and Bacteroides strains. These results may suggest that resistant dextrins exerted a prebiotic-like effect in the large intestine.

Keywords

EN

resistant dextrin; microflora; rats

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 4
• Pages: 677-681

Dates

published

2015

received

2015-07-15

revised

2015-09-17

accepted

2015-10-05

(unknown)

2015-11-27

Contributors

author

Katarzyna Śliżewska

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Lodz, Poland

author

Zdzisława Libudzisz

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Lodz, Poland

author

Renata Barczyńska

• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Czestochowa, Czestochowa, Poland

author

Janusz Kapuśniak

• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Czestochowa, Czestochowa, Poland

author

Zenon Zduńczyk

• Insitute of Animal Reproduction and Food Research, Polish Academy of Science in Olsztyn, Olsztyn, Poland

author

Jerzy Juśkiewicz

• Insitute of Animal Reproduction and Food Research, Polish Academy of Science in Olsztyn, Olsztyn, Poland

References

• Azad MB, Konya T, Guttman DS, Field CJ, Sears MR, HayGlass KT, Mandhane PJ, Turvey SE, Subbarao P, Becker AB, Scott JA, Kozyrskyj AL, CHILD Study Investigators (2015) Infant gut microbiota and food sensitization: associations in the first year of life. Clin Exp Allergy 45: 632-643.
• Berard M, Deremaux L, Lefranc-Millot C (2009) Prebiotic effects of Nutriose®: a review. International Congress of Nutrition 4-9 October 2009, Bangkok, Thailand.
• Berenal MJ, Periago MJ, Ros G (2002) Effects of processing on dextrin, total starch, dietary fiber and starch digestibility in infant. J Food Sci 67: 1249-1254.
• Blaut M, Clavel T (2007) Metabolic diversity of the intestinal microbiota: implications for health and disease. J Nutr 137: 751S-755S.
• Guarner F, Malagelada JR (2003) Gut flora in health and disease. Lancet 361: 512-519.
• Hofirek B, Haas D (2001) Comparative studies of ruminal fluid collected by oral tube or by puncture of the caudorental ruminal of the caudorental ruminal sac. Acta Vet (Brno) 70: 27-33.
• Hong J, He M, Yang YX, Shen X (2005) Influence of resistant starch on colon flora of rats. J Hygiene Res 34: 85-87.
• Kapusniak J, Barczynska R, Slizewska K, Libudzisz Z (2008) Utilization of enzyme-resistant chemically modified dextrins from potato starch by Lactobacillus bacteria. Zesz Prob Postępów Nauk Rol 530: 445-457 (in Polish).
• Kleessen B, Bezirtzoglou E, Matto J (2000) Culture-based knowledge on biodiversity, development and stability of human gastrointestinal microflora. Microb Ecol Health Dis 12: 53-63.
• Kleessen B, Stoof G, Proll J, Schmiedl D, Noack J, Blaut M (1997) Feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats. J Anim Sci 75: 2453-2462.
• Koboziev I, Reinoso W, Furr KL, Grisham MB (2014) Role of the enteric microbiota in intestinal homeostasis and inflammation. Free Radic Biol Med 68: 122-133.
• Kordyl M (2010) Ability of intestinal microorganisms to metabolize prebiotic preparations. Ph.D. Thesis, Lodz University of Technology, 2010 (in Polish).
• Martinez I, Kim J, Duffy PR, Schlegel VL, Walter J (2010) Resistant starches types 2 and 4 have differential effects on the composition of the fecal microbiota in human subject. Plos One 5: e15046.
• Ohkuma K, Matsuda I, Katta Y, Hanno Y (1999) Pyrolysis of starch and its digestibility by enzymes - Characterization of indegestible dextrin. Denpun Kagaku 37: 107-114.
• Ohkuma K, Wakabayashi S (2001) Fibersol-2: A soluble, non-digestible, starch-derived dietary fibre. In Advanced Dietary Fibre Technology. McCleary BV, Prosky L, eds, pp 509-523. Blackwell Science Ltd, Oxford, United Kingdom.
• Olano-Martin E, Gibson GR, Rastell RA (2002) Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides. J Appl Microbiol 93: 505-511.
• Palframan R, Gibson GR, Rastall RA (2003) Development of a quantitative tool for the comparison of the prebiotic effect of dietary oligosaccharides. Lett Appl Microbiol 37: 281-284.
• Pasman W, Wils D, Saniez MH, Kardinaal A (2006) Long-term gastrointestinal tolerance of Nutriose FB in healthy men. Eur J Clin Nutr 60: 1024-1034.
• Preidis GA, Versalovic J (2009) Targeting the human microbiome with antibiotics, probiotics and prebiotics: gastroenterology enters the metagenomics era. Gastroenterology 136: 2015-2031.
• Wang YJ, Kozlowski R, Delgado GA (2001) Enzyme resistant dextrins from high amylose corn mutant starches. Starch/Stärke 53: 21-26.

Identifiers

DOI

10.18388/abp.2015_1101