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2015 | 62 | 3 | 613-619
Article title

Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly

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The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated Protein 55kDa (RAP55/LSM14) during mitosis was identified as a Mitotic Spindle Protein (MSP). We found a new location obtained by expressing GFP-tagged proteins in HeLa Cells during mitosis that has never been previously reported. We demonstrated also, for the first time, that the depletion of RAP55/LSM14 destabilizes spindle assembly, stops cells in mitosis and induces many other cell cytoskeletal disorders. Finally, by using an "in vitro" assay investigation, we found that RAP55/LSM14 binds directly the tubulin and that is implicated in the process of the mitotic spindle stabilization, which is a novel discovery in this field of research.
Physical description
  • Inserm Unité 836, 38043 Grenoble, France
  • UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie
  • Inserm Unité 836, 38043 Grenoble, France
  • UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie
  • Bakhoum SF, Thompson SL, Manning AL, Compton DA (2009) Nature Cell Biol 11: 27-35.
  • Blower MD, Brown KS, Maresca TJ, Grammer TC, Harland RM, Heald R (2005) J Cell Biol 176: 765.
  • Blower MD, Feric E, Weis K, Heald R (2007) J Cell Biol 179: 1365-1373.
  • Cai P, Lu P, Sharom FJ, Fang WS (2013) Cancer Lett 341: 214-223.
  • Castoldi M, Popov AV (2003) Protein Expr Purif 32: 83-88.
  • Cha BJ, Cassimeris L, Gard DL (1999) J Cell Sci 112: 4337-4346.
  • Craddock TJ, Beauchemin C, Tuszynski JA (2009) BioSystems 97: 28-34.
  • Dominguez R, Holmes KC (2011) Annu Rev Biophys 40: 169-186.
  • Gache V, Waridel P, Luche S, Shevchenko A, Popov AV (2007) Methods Mol Med 137: 29-43.
  • Gache V, Waridel P, Winter C, Juhem A, Schroeder M, Shevchenko A, Popov AV (2010) PLoS One 5: e9248.
  • Groisman I, Huang YS, Mendez R, Cao Q, Theurkauf W, Richter JD (2000) Cell 103: 435-447.
  • Gumireddy K, Ramana MVR, Cosenza SC, Nathan RB, Baker SJ, Papathi N, Jiang J, Holland J, Reddy EP (2005) Cancer Cell 7: 497.
  • Liska AJ, Popov AV, Sunyaev S, Coughlin P, Habermann B, Shevchenko A, Bork P, Karsenti E, Shevchenko A (2004) Proteomics 4: 2707-2721.
  • Maccioni RB, Cambiazo V (1995) Physiol Rev 75: 835-864.
  • Magalhaes HI, Wilke DV, Bezerra DP, Cavalcanti BC, Rotta R, de Lima DP, Beatriz A, Moraes MO, Diniz-Filho J, Pessoa C (2013) Toxicol Appl Pharmacol 272: 117-126.
  • Marnef A, Sommerville J, Ladomery MR (2009) Int J Biochem Cell Biol 41: 977-981.
  • Merdes A, Ramyar K, Vechio JD, Cleveland DW (1996) Cell 87: 447-458.
  • Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Edde B (2003) J Cell Sci 116: 4181-4190.
  • Sawin KE, LeGuellec K, Philippe M, Mitchison TJ (1992) Nature 359: 540-543.
  • Simpson JC, Nilsson T, Pepperkok R (2006) Mol Biol Cell 17: 723-737.
  • Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2000) EMBO Rep 1: 287-292.
  • Tanaka KJ, Ogawa K, Takagi M, Imamoto N, Matsumoto K, Tsujimoto M (2006) J Biol Chem 281: 40096-40106.
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