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2015 | 62 | 3 | 613-619
Article title

Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly

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EN
Abstracts
EN
The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated Protein 55kDa (RAP55/LSM14) during mitosis was identified as a Mitotic Spindle Protein (MSP). We found a new location obtained by expressing GFP-tagged proteins in HeLa Cells during mitosis that has never been previously reported. We demonstrated also, for the first time, that the depletion of RAP55/LSM14 destabilizes spindle assembly, stops cells in mitosis and induces many other cell cytoskeletal disorders. Finally, by using an "in vitro" assay investigation, we found that RAP55/LSM14 binds directly the tubulin and that is implicated in the process of the mitotic spindle stabilization, which is a novel discovery in this field of research.
Keywords
Publisher

Year
Volume
62
Issue
3
Pages
613-619
Physical description
Dates
published
2015
received
2015-07-11
revised
2015-08-10
accepted
2015-08-11
(unknown)
2015-09-04
Contributors
author
  • Inserm Unité 836, 38043 Grenoble, France
  • UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie
  • Inserm Unité 836, 38043 Grenoble, France
  • UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p613kz
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