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2015 | 62 | 3 | 605-612
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Antibacterial activity of caffeine against plant pathogenic bacteria

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The objective of the present study was to evaluate the antibacterial properties of a plant secondary metabolite - caffeine. Caffeine is present in over 100 plant species. Antibacterial activity of caffeine was examined against the following plant-pathogenic bacteria: Ralstonia solanacearum (Rsol), Clavibacter michiganesis subsp. sepedonicus (Cms), Dickeya solani (Dsol), Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pseudomonas syringae pv. tomato (Pst), and Xanthomonas campestris subsp. campestris (Xcc). MIC and MBC values ranged from 5 to 20 mM and from 43 to 100 mM, respectively. Caffeine increased the bacterial generation time of all tested species and caused changes in cell morphology. The influence of caffeine on the synthesis of DNA, RNA and proteins was investigated in cultures of plant pathogenic bacteria with labelled precursors: [3H]thymidine, [3H]uridine or 14C leucine, respectively. RNA biosynthesis was more affected than DNA or protein biosynthesis in bacterial cells treated with caffeine. Treatment of Pba with caffeine for 336 h did not induce resistance to this compound. Caffeine application reduced disease symptoms caused by Dsol on chicory leaves, potato slices, and whole potato tubers. The data presented indicate caffeine as a potential tool for the control of diseases caused by plant-pathogenic bacteria, especially under storage conditions.
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  • Agarwal PC, Mortensen CN, Mathur SB (1989) Seedborne disease and seed health testing of rice. In Technical Bulletin 3. Phytopathol Pap 30. Danish Government Institute of Seed Pathology for Developing Countries and CAB International Mycological Institute.
  • Ashihara H (2006) Metabolism of alkaloids in coffee plants. Brazil J Plant Physiol 18: 1-8.
  • Ashihara H, Sano H, Crozier A (2008) Caffeine and related purine alkaloids: Biosynthesis, catabolism, function and genetic engineering. Phytochem 69: 841-856.
  • Avery ML, Werner SJ, Cummings JL, Humphrey JS, Milleson MP, Carlson J C, Primus TM, Goodall MJ (2005) Caffeine for reducing bird damage to newly seeded rice. Crop Prot 24: 651-657.
  • Bertani G (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62: 293-300.
  • Cano-Marquina A, Tarín JJ, Cano A (2013) The impact of coffee on health. Maturitas 75: 7-21.
  • Cogo K, Montan MF, Bergamaschi C, Andrade ED, Rosalen PL, Groppo FC (2008) In vitro evaluation of the effect of nicotine, cotinine and caffeine on oral microorganisms. Can J Microbiol 54: 501-508.
  • Croteau R, Kutchan TM, Lewis NG (2000) Natural products (secondary metabolites). In Biochemistry and Molecular Biology of Plants: 1250-1318. American Society of Plant Physiologists. Rockville, MD.
  • Czajkowski R, Perombelon MCM, van Veen JA, van der Wolf JM (2011) Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathol 60: 999-1013.
  • De la Cruz AR, Wiese MV, Schaad NW (1992) A semiselective agar medium for isolation of Clavibacter michiganensis subsp. sepedonicus from potato tissues. Plant Dis 76: 830-834.
  • French ER, Gutarra L, Aley P, Elphinstone J (1995) Culture media for Pseudomonas solanacearum isolation, identification and maintenance. Fitopatol 30: 126-130.
  • Hollingsworth RG, Armstrong JW, Campbell E (2002) Caffeine as a repellent for slugs and snails. Nature 417: 915-916.
  • Kim YS, Sano H (2008) Pathogen resistance of transgenic tobacco plants producing caffeine. Phytochemistry 69: 882-888.
  • Labbe RG, Nolan LL (1987) Inhibition of macromolecular synthesis by caffeine in Clostridium perfringens. Can J Microbiol 33: 589-592.
  • Lieb M (1961) Enhancement of ultraviolet-induced mutation in bacteria by caffeine. Mol Gen Genet 92: 416-429.
  • Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, Dow M, Verdier V, Beer SV, Machado MA, Toth I, Salmond G, Foster GD (2012) Top 10 plant pathogenic bacteria in molecular plant pathology. Mol Plant Pathol 13: 614-629.
  • Mohammed MJ, Al-Bayati FA (2009) Isolation, identification and purification of caffeine from Coffea arabica L. and Camellia sinensis L.: A combination antibacterial study. Int J Green Pharm 3: 52-57.
  • Nathanson JA (1984) Caffeine and related methylxanthines: possible naturally occurring pesticides. Science 226: 184-187.
  • Raj CV, Dhala S (1965) Effect of naturally occurring xanthines on bacteria. I. Antimicrobial action and potentiating effect on antibiotic spectra. Appl Microbiol 13: 432-436.
  • Ramanaviciene A, Mostovojus V, Bachmatova I, Ramanavicius A (2003) Anti-bacterial effect of caffeine on Escherichia coli and Pseudomonas fluorescens. Acta Med Lit 10: 185-188.
  • Sandlie I, Solberg K, Kleppe K (1980) The effect of caffeine on cell growth and metabolism of thymidine in Escherichia coli. Mutat Res 73: 29-41.
  • Summers RM, Seffernick JL, Quandt EM, Yu CL, Barrick JE, Subramanian M V (2013) Caffeine junkie: an unprecedented glutathione S-transferase-dependent oxygenase required for caffeine degradation by Pseudomonas putida CBB5. J Bacteriol 195: 3933-3939.
  • Szpitter A, Narajczyk M, Maciag-Dorszynska M, Wegrzyn G, Lojkowska E, Krolicka A (2014) Effect of Dionaea muscipula extract and plumbagin on maceration of potato tissue by Pectobacterium atrosepticum. Ann App Biol 164: 404-414.
  • Thornsberry C (1991) Antimicrobial susceptibility testing: general considerations. In Manual of Clinical Microbiology, pp 1059-1201. Washington DC, American Society for Microbiology.
  • Wegrzyn G, Kwasnik E, Taylor K (1991) Replication of λ plasmid in amino acid-starved strains of Escherichia coli. Acta Biochim Pol 38: 181-186.
  • Zheng S, Liu Q, Zhang G, Wang H, Ng TB (2014) Purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom Clitocybe sinopica. Acta Biochim Pol 57: 43-48.
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