1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii atcc 8090

• Authors: Hanna Przystałowska , Joanna Zeyland , Alicja Kośmider , Marlena Szalata , Ryszard Słomski , Daniel Lipiński
• Languages of publication: EN

Abstracts

EN

Compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in the production of various industrial chemicals. As a valuable organic compound, 1,3-propanediol (1,3-PDO) has numerous applications in the production of polymers, lubricants, cosmetics and medicines. Here, conversion of glycerol (a renewable substrate and waste from biodiesel production) to 1,3-PDO by E. coli bacterial strain carrying altered glycerol metabolic pathway was investigated. Two gene constructs containing the 1,3-PDO operon from Citrobacter freundii (pCF1 and pCF2) were used to transform the bacteria. The pCF1 gene expression construct contained dhaBCE genes encoding the three subunits of glycerol dehydratase, dhaF encoding the large subunit of the glycerol dehydratase reactivation factor and dhaG encoding the small subunit of the glycerol dehydratase reactivating factor. The pCF2 gene expression construct contained the dhaT gene encoding the 1,3-propanediol dehydrogenase. Expression of the genes cloned in the above constructs was under regulation of the T7lac promoter. RT-PCR, SDS-PAGE analyses and functional tests confirmed that 1,3-PDO synthesis pathway genes were expressed at the RNA and protein levels, and worked flawlessly in the heterologous host. In a batch flask culture, in a short time applied just to identify the 1,3-PDO in a preliminary study, the recombinant E. coli bacteria produced 1.53 g/L of 1,3-PDO, using 21.2 g/L of glycerol in 72 h. In the Sartorius Biostat B Plus reactor, they produced 11.7 g/L of 1,3-PDO using 24.2 g/L of glycerol, attaining an efficiency of 0.58 [mol1,3-PDO/molglycerol].

Keywords

EN

bio-based 1,3-propanediol; genetically modified bacteria; glycerol utilization; renewable resources; Citrobacter freundii; Escherichia coli

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 3
• Pages: 589-597

Dates

published

2015

received

2015-05-14

revised

2015-06-28

accepted

2015-08-03

(unknown)

2015-09-08

Contributors

author

Hanna Przystałowska

• Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland

author

Joanna Zeyland

• Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland

author

Alicja Kośmider

• Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poznań, Poland

author

Marlena Szalata

• Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland
• Polish Academy of Sciences, Institute of Human Genetics, Poznań, Poland

author

Ryszard Słomski

• Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland
• Polish Academy of Sciences, Institute of Human Genetics, Poznań, Poland

author

Daniel Lipiński

• Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland
• Polish Academy of Sciences, Institute of Human Genetics, Poznań, Poland

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Identifiers

DOI

10.18388/abp.2015_1061