PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 3 | 563-567
Article title

Clastogenic and toxicological assessment of cashew (Anacardium occidentale) nut bark extracts in Wistar rats

Content
Title variants
Languages of publication
EN
Abstracts
EN
Occupational exposures to environmental toxicants have been associated with the onset of skin lesions-including cancers. Identification and reduction of exposure to such compounds is an important public health goal. We examined the effect of cashew shell oil (CSO), used in skin tattooing for its potential to induce skin transformation in rats. Corn oil and CSO (25, 50, and 100%) were topically applied to depilated sections of Wistar' rat skin (groups: I-IV) for six weeks. Effect of treatments on serum transaminases activity, histological changes in hepatocytes and induction of micronuclei in the bone marrow were examined. In addition, CSO-induced hepatocyte proliferation was also quantified. All animals survived the course of the study. Reduced percentage change in body weight and physical trauma were observed in CSO-treated rat. The effects were more prominent in Group IV (100% CSO). Relative liver weights and number of hepatocytes (cells/mm2) increased significantly in groups II-IV relative to control (p < 0.05). Serum transaminases activities were not significantly (p > 0.05) affected in treated groups. Hepatic histopathology revealed moderate sinusoidal congestion (group II), in addition to portal congestion in (group III), with mononuclear cellular infiltration (group IV) animals. In addition, CSO induced significant micronuclei formation of polychromatic erythrocyte (mPCEs) in the rat bone marrow (p < 0.05) when compared with control. Topical application of CSO disrupted skin cells integrity resulting in physical trauma. In addition, CSO appears to be clastogenic and induces hepatocyte proliferation. Occupational exposure to CSO especially for engraving tattoos in humans should be discouraged and further studies need to be conducted.
Year
Volume
62
Issue
3
Pages
563-567
Physical description
Dates
published
2015
received
2015-04-08
revised
2015-07-10
accepted
2015-08-05
(unknown)
2015-09-02
References
  • Aber R, Marks JG, DeMeIfi T (1983) Dermatitis associated with cashew nut consumption - Pennsylvania. MMWR 32: 129-130.
  • Argyris TS (1989) Epidermal tumor promotion by damage in the skin of mice. Prog Clin Biol Res 298: 63-80.
  • Bonassi S, Znaor A, Ceppi M, Lando C, Chang WP, Holland N (2006) An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 28: 625-631.
  • Celik A, Ogenler O, Comelekoglu U (2005) The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. Mutagenesis 20: 411-415.
  • Downing JG, Gurney SW (1940) Dermatitis from cashew nut shell oil. J Ind Hyg Toxico 22: 169-174.
  • George J, Kuttan R (1997) Mutagenic, carcinogenic and cocarcinogenic activity of cashewnut shell liquid. Cancer Lett 112: 11-16.
  • Hecker E, Adolf W, Hergenhahn M, Schmidt R, Sorg B (1984) Irritant diterpene ester promoters of mouse skin: Contributions to etiologies of environmental cancer and to biochemical chanisms of carcinogenesis. In Cellular Interactions by Environmental Tumour Promoters, pp 3-36. Japan Science and Social Press/VUN Science Press: Tokyo/Utrecht.
  • Heddle JA, Salamone MF (1981) The micronucleus assay I: In vitro. In: Topics in environmental physiology and medicine. Short-term test for chemical carcinogens. Stich HF, San RHC, eds, pp 243-249. New York. Springer-Verlag.
  • Hochegger K, Siebenhaar F, Vielhauer V, Heininger D, Mayadas TN, Mayer G, Maurer M, Rosenkranz AR (2005) Role of mast cells in experimental anti-glomerular basement membrane glomerulonephritis. Eur J Immunol 35: 3074-3082.
  • Ideo G, Morganti A, Dioguardi N (1972) Gamma-glutamyl transpeptidase: a clinical and experimental study. Digestion 5: 326-336.
  • Lum G, Gambino SR (1972) Serum gamma-glutamyl transpeptidase activity as an indicator of disease of liver, pancreas, or bone. Clin Chem 18: 358-362.
  • Marks JG, DeMelfi T, McCarthy MA, Witte EJ, Castagnoli N, Epstein WL, Aber RC (1984) Dermatitis from cashew nuts. J Am Acad Dermatol 10: 627-631.
  • Morton JF (1961) The cashews brighter future. Econ Bot 15: 57-78.
  • Ojeh OA (1981) Production and utilisation of cashewnut shell liquid in Nigeria. Cashew Causerie (April-June): 9-12.
  • Orris L (1958) Cashew nut dermatitis. N Y State J Med 58: (17 Part 1) 2799-2800.
  • Polasa K, Rukmini C (1987) Mutagenicity tests of cashewnut shell liquid, rice-bran oil and other vegetable oils using the Salmonella typhimurium/microsome system. Food Chem Toxicol 25: 763-766.
  • Ratner JH, Spencer SK, Grainge JM (1974) Cashew nut dermatitis. An example of internal-external contact-type hypersensitivity. Arch Dermatol 110: 921-923.
  • Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28: 56-63.
  • Sullivan JT, Richards CS, Lloyd HA, Krishna G (1982) Anacardic acid: molluscicide in cashew nut shell liquid. Planta Med 44: 175-177.
  • Trosko JE (2003) The role of stem cells and gap junctional intercellular communication in carcinogenesis. J Biochem Mol Biol 36: 43-48.
  • Varghese I, Rajendran R, Sugathan CK, Vijayakumar T (1986) Prevalence of oral submucous fibrosis among the cashew workers of Kerala-south India. Indian J Cancer 23: 101-104.
  • Yuan J, Jameson CW, Goehl TJ, Elwell MR, Leininger JR, Thompson MB, Corniffe G, Carlton T (1992) Application of molecular encapsulation for toxicology studies: comparative toxicity of p-Chloro-alpha, alpha, alpha-trifluorotoluene in alpha-cyclodextrin vehicle versus corn oil vehicle in male and female Fischer 344 rats and B6C3F1 mice. Fundam Appl Toxicol 18: 460-470.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p563kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.