Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis

• Authors: Emília Hijová , Alena Šoltésová , Rastislav Salaj , Jozef Kuzma , Ladislav Strojný , Alojz Bomba , Kristína Gregová
• Languages of publication: EN

Abstracts

EN

The aim of presented study was to investigate the influence of Lactobacillus plantarum LS/07 and inulin on the activity of β-glucuronidase enzyme, and counts of coliform and lactobacilli in fresh caecal digesta, cytokine levels (IL-6, IL-8), and trancription nuclear factor kappa beta (NFκB) activities in colon tissue and blood samples of rats with dextran sulphate sodium (DSS) induced acute colitis. The rats were randomly divided into four groups - CG, AC, AC+PRE and AC+PRO. Colitis was induced using of 5% DSS in drinking water for 7d. DSS application increased activity of β-glucuronidase (P < 0.001), increased counts of coliforms, and decreased lactobacilli counts (P < 0.05) in comparison to control group. Serum and tissue levels of IL-6 and IL-8 as well as tissue NFκB activities showed increased expression in acute colitis group. Inulin diet modified counts of microorganims and decreased β-glucuronidase activity, suppressed NFκB activities (P < 0.001) and down regulate synthesis of IL-6 (P < 0.01) in serum and colon tissue and tissue IL-8 (P < 0.05). Lactobacillus plantarum LS/07 decreased β-glucuronidase activity (P < 0.05), levels of IL-6 and IL-8 (P < 0.001). These results were consistent with the addition of histological findings. Our results indicate that dietary intake of Lactobacillus plantarum LS/07 and inulin suppressed expression observed markers, which play an important role in the inflammatory process, which predisposes their use in prevention or treatment of acute colitis.

Keywords

EN

colitis; Sprague-Dawley rats; inulin; Lactobacillus plantarum LS/07

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 3
• Pages: 553-557

Dates

published

2015

received

2015-03-16

revised

2015-07-21

accepted

2015-08-07

(unknown)

2015-09-08

Contributors

author

Emília Hijová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Alena Šoltésová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Rastislav Salaj

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Jozef Kuzma

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Ladislav Strojný

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Alojz Bomba

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Kristína Gregová

• Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

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Identifiers

DOI

10.18388/abp.2015_1008