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2015 | 62 | 3 | 457-463
Article title

Splenic melanosis in agouti and black mice

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EN
Abstracts
EN
An interesting example of extradermal deposition of melanin in vertebrates, notably in mammals, is splenic melanosis. In particular, if the phenomenon of splenic melanosis is correlated with hair or skin pigmentation, it must reflect the amount and perhaps the quality of pigment produced in hair follicle melanocytes. The present paper is our first study on splenic pigmentation in mice of phenotype agouti. We used untreated mixed background mice C57BL/6;129/SvJ (black - a/a, agouti - A/a, A/A), and as a control - black C57BL/6 and agouti fur from 129/SvJ mice, Mongolian gerbils (Meriones unguiculatus) and golden hamsters (Mesocricetus auratus). After euthanasia skin and spleen was evaluated macroscopically, photographed and collected for further analysis using Fontana-Masson and hematoxylin-eosin staining and electron paramagnetic resonance (EPR) at X-band. Spleens of the agouti mice revealed splenic melanosis but were slightly weaker pigmented than their black counterparts, while the presence of pheomelanin was difficult to determine. The fur of both phenotypes was of similar melanin content, with the same tendency as in the spleens. The contribution of pheomelanin in the agouti fur was on the border of detectability by EPR. Histological and EPR analysis confirmed the presence of melanin in the melanotic spleens. The shape of the EPR signal showed a dominance of eumelanin in fur and in melanized spleens in both phenotypes of mice. Therefore, splenic melanosis does reflect the hair follicle pigmentation not only in black, but also in agouti mice.
Keywords
EN
Publisher

Year
Volume
62
Issue
3
Pages
457-463
Physical description
Dates
published
2015
received
2015-04-13
revised
2015-05-05
accepted
2015-05-21
(unknown)
2015-08-20
Contributors
  • Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
References
  • Bultman S, Michaud E, Woychik R (1992) Molecular characterization of the mouse agouti locus. Cell 71: 1195-1204.
  • Chikvaidze EN, Partskhaladze TM, Gogoladze TV (2014) Electron spin resonance (ESR/EPR) of free radicals observed in human red hair: a new, simple empirical method of determination of pheomelanin/eumelanin ratio in hair. Magn Reson Chem 52: 377-382.
  • Commoner B, Townsend J, Pake GW (1954) Free radicals in biological materials. Nature 174: 689-691.
  • Chase HB (1954) Growth of the hair. Physiol Rev 34: 113-126.
  • Chase HB, Eaton GJ (1959) The growth of hair follicles in waves. Ann N Y Acad Sci 83: 365-368.
  • Crichton DN, Busuttil A, Price WH (1978) Splenic lipofuscinosis in mice. J Pathol 126: 113-120.
  • Davies KJ, Hochstein P (1982) Ubisemiquinone radicals in liver: implications from a mitochondrial Q cycle in vivo. Biochem Biophys Res Commun 107: 1292-1299.
  • Dry FW (1926) The coat of the mouse (Mus musculus). J Genet 16: 281-340.
  • Elas M, Bielanska J, Pustelny K, Plonka PM, Drelicharz L, Skorka T, Tyrankiewicz U, Wozniak M, Heinze-Paluchowska S, Walski M, Wojnar L, Fortin D, Ventura-Clapier R, Chlopicki S (2008) Detection of mitochondrial dysfunction by EPR technique in mouse model of dilated cardiomyopathy. Free Radic Biol Med 45: 321-328.
  • Hill HZ, Hill GJ, Cieszka K, Plonka PM, Mitchell DL, Meyenhofer MF, Xin P, Boissy RE (1997) Comparative action spectrum for ultraviolet light killing of mouse melanocytes from different genetic coat color backgrounds. Photochem Photobiol 65: 983-989.
  • Ito S, Fujita K (1985) Microanalysis of eumelanin and pheomelanin in hair and melanomas by chemical degradation and liquid chromatography. Anal Biochem 144: 527-536.
  • Ito S, Wakamatsu K (2003) Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: a comparative review. Pigment Cell Res 16: 523-531.
  • Kaelin CB, Candille SI, Yu B, Jackson P, Thompson DA, Nix MA, Binkley J, Millhauser GL, Barsh GS (2008) New ligands for melanocortin receptors. Int J Obes (Lond) 32: S19-S27.
  • Kubiak T, Krzyminiewski R, Dobosz B (2013) EPR study of paramagnetic centers in human blood. Curr Top Biophys 36: 7-13.
  • Land EJ, Ramsden CA, Riley PA (2004) Quinone chemistry and melanogenesis. Methods Enzymol 378: 88-109.
  • Linden M, Ward JM, Cherian S (2012) Hematopoietic and lymphoid tissues. In Comparative anatomy and histology. A mouse and human atlas. Treuting MP, Dintzis SM, eds. pp 309-338. Academic Press @ Elsevier, Amsterdam, Boston, Heidelberg, London, New York, Oxford, Paris, San Diego, San Francisco, Singapore, Sydney, Tokyo.
  • Linder CC (2003) Mouse nomenclature and maintenance of genetically engineered mice. Comp Med 53: 119-125.
  • Meredith P, Sarna T (2006) The physical and chemical properties of eumelanin. Pigment Cell Res 19: 572-594.
  • Michalczyk D, Popik M, Salwinski A, Plonka PM (2009) Extradermal melanin transfer? Lack of macroscopic spleen melanization in old C57BL/6 mice with de-synchronized hair cycle. Acta Biochim Pol 56: 343-353.
  • Michalczyk-Wetula D, Salwiński A, Popik M, Jakubowska M, Plonka PM (2013) Splenic melanosis during normal murine C57BL/6 hair cycle and after chemotherapy. Acta Biochim Pol 60: 313-321.
  • Militzer K (2001) Hair growth pattern in nude mice. Cells Tissues Organs 168: 285-294.
  • Millar SE, Miller MW, Stevens ME, Barsh GS (1995) Expression and transgenic studies of the mouse agouti gene provide insight into the mechanisms by which mammalian coat color patterns are generated. Development 121: 3223-3232.
  • Ollmann MM, Lamoreux LL, Wilson BD, Barsh GS (1998) Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Gene Dev 12: 316-330.
  • Oyehaug L, Plahte E, Vage DI, Omholt SW (2002) The regulatory basis of melanogenic switching. J Theor Biol 215: 449-468.
  • Ozeki H, Ito S, Wakamatsu K, Hirobe T (1995) Chemical characterization of hair melanins in various coat-color mutants of mice. J Invest Dermatol 105: 361-366.
  • Ozeki H, Ito S, Wakamatsu K, Thody AJ (1996) Spectrophotometric characterization of eumelanin and pheomelanin in hair. Pigment Cell Res 9: 265-270.
  • Paus R, Christoph T, Müller-Röver S (1999) Immunology of the hair follicle: a short journey into terra incognita. J Investig Dermatol Symp Proc 4: 226-234.
  • Plonka PM (2009) Electron paramagnetic resonance as a unique tool for skin and hair research. Methods review. Exp Dermatol 18: 472-484.
  • Plonka PM, Michalczyk D, Popik M, Handjiski B, Slominski A, Paus R (2005) Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. Acta Biochim Pol 52: 433-441.
  • Plonka PM, Michalczyk D, Popik M, Handjiski B, Paus R (2008) Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after a spontaneous or depilation-induced hair growth. J Dermatol Sci 49: 227-240.
  • Plonka PM, Passeron T, Brenner M, Tobin D, Shibahara S, Thomas A, Slominski A, Kadekaro AL, Hershkovitz D, Peters E, Nordlund JJ, Abdel-Malek Z, Takeda K, Paus R, Ortonne JP, Hearing VJ, Schallreuter K (2009) What are melanocytes really doing all day long...? Exp Dermatol 18: 799-819.
  • Plonka P, Plonka B, Paus R (1995) Biophysical monitoring of melanogenesis as a tool for pigment and hair research. Arch Dermatol Res 287: 687-690.
  • Plonka PM, Slominski AT, Pajak S, Urbanska K (2003) Transplantable melanomas in gerbils (Meriones unguiculatus). II - Melanogenesis. Exp Dermatol 12: 356-364.
  • Sakurai T, Ochiai H, Takeuchi T (1975) Ultrastructural change of melanosomes associated with agouti pattern formation in mouse hair. Dev Biol 47: 466-471.
  • Schallreuter KU, Kothari S, Chavan B, Spencer JD (2008) Regulation of melanogenesis - controversies and new concepts. Exp Dermatol 17: 395-404.
  • Sealy RC, Hyde JS, Felix CC, Menon IA, Prota G, Swartz HM, Persad S, Haberman HF (1982) Novel free radicals in synthetic and natural pheomelanins: distinction between dopa melanins and cysteinyldopa melanins by ESR spectroscopy. Proc Natl Acad Sci USA 79: 2885-2889.
  • Slominski A, Goodman-Snitkoff G, Maurer M, Paus R (1997) Hair cycle-associated changes in splenocyte proliferation. In Vivo 11: 101-102.
  • Slominski A, Paus R (1993) Melanogenesis is coupled to murine anagen: towards new concepts for the role of melanocytes and the regulation of melanogenesis in hair growth. J Invest Dermatol 101: 90S-97S.
  • Slominski A, Paus R, Plonka P, Chakraborty A, Maurer M, Pruski D, Lukiewicz S (1994) Melanogenesis during the anagen-catagen-telogen transformation of the murine hair cycle. J Invest Dermatol 102: 862-869.
  • Slominski A, Plonka PM, Pisarchik A, Smart JL, Tolle V, Wortsman J, Low MJ (2005a) Preservation of eumelanin hair pigmentation in proopiomelanocortin-deficient mice on a nonagouti (a/a) genetic background. Endocrinology 146: 1245-1253.
  • Slominski A, Tobin D J, Shibahara S, Wortsman J (2004) Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev 84: 1155-1228.
  • Slominski A, Wortsman J, Plonka PM, Schallreuter KU, Paus R, Tobin DJ (2005b) Hair follicle pigmentation. J Invest Dermatoli 124: 13-21.
  • Threadgill DW, Yee D, Matin A, Nadeau JH, Magnuson T (1997) Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm Genome 8: 390-393.
  • Vachtenheim J, Borovanský J (2010) 'Transcription physiology' of pigment formation in melanocytes: central role of MITF. Exp Dermatol 19: 617-627.
  • van der Heijden A, van Dijk JE, Lemmens AG, Beynen AC (1995) Spleen pigmentation in young C57BL mice caused by accumulation of melanin. Lab Anim 29: 459-463.
  • Vsevolodov EB, Ito S, Wakamatsu K, Kuchina II, Latypov IF (1991) Comparative analysis of hair melanins by chemical and electron spin resonance methods. Pigment Cell Res 4: 30-34.
  • Weissman I (1967) Genetic and histochemical studies on mouse spleen black spots. Nature 215: 315.
  • Wolnicka-Glubisz A, Pecio A, Podkowa D, Kolodziejczyk LM, Plonka PM (2011) Pheomelanin in the skin of Hymenochirus boettgeri (Amphibia: Anura: Pipidae). Exp Dermatol 21: 537-540.
  • Wood JM, Jimbow K, Boissy RE, Slominski A, Plonka PM, Slawinski J, Wortsman J, Tosk J (1999) What's the use of generating melanin? Exp Dermatol 8: 153-164.
  • Zieri R, Franco-Belussi L, de Souza Santos LR, Taboga SR, de Oliveira C (2015) Sex hormones change visceral pigmentation in Eupemphix nattereri (Anura): effects in testicular melanocytes and hepatic melanomacrophages. Anim Biol 65: 21-32.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p457kz
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