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2015 | 62 | 3 | 395-400
Article title

Wheat straw degradation and production of alternative substrates for nitrogenase of Rhodobacter sphaeroides

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Cellulose is a major component of plant biomass and could be applied in the production of biofuels, especially bioethanol. An alternative approach is production of a clean fuel - hydrogen from cellulosic biomass. In this paper an innovatory model of cellulosic waste degradation has been proposed to verify the possibility of utilization of cellulose derivatives by purple non-sulfur bacteria. The concept is based on a two-step process of wheat straw conversion by bacteria in order to obtain an organic acid mixture. In the next stage such products are consumed by Rhodobacter sphaeroides, the known producer of hydrogen. It has been documented that Cellulomonas uda expresses cellulolytic activity in the presence of wheat straw as an only source of carbon. R. sphaeroides applied in this research can effectively consume organic acids released from straw by C. uda and Lactobacillus rhamnosus and is able to grow in the presence of these substrates. Additionally, an increased nitrogenase activity of R. sphaeroides has been indicated when bacteria were cultivated in the presence of cellulose derivatives which suggests that hydrogen production occurs.

Physical description
  • Department of Plant Physiology and Development, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Development, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Akkerman I, Janssen M, Rocha J, Wijffels RH (2002) Photobiological hydrogen production: Photochemical efficiency and bioreactor design. Int J Hydrogen Energy 27: 1195-1208.
  • Asada Y, Ohsawa M, Nagai Y, Ishimi K, Fukatsu M, Hideno A, Wakayama T, Miyake J (2008) Re-evaluation of hydrogen productivity from acetate by some photosynthetic bacteria. Int J Hydrogen Energy 33: 5147-5150.
  • Basak N, Das D (2007) The prospect of purple non-sulfur (PNS) photosynthetic bacteria for hydrogen production: the present state of the art. World J Microbiol Biotechnol 23: 31-42.
  • Cui F, Li Y, Wan C (2011) Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis. Bioresource Technol 102: 1831-1836.
  • Desvaux M, Guedon E, Petitdemange H (2001) Kinetics and metabolism of cellulose degradation at high substrate concentrations in steady-state continuous cultures of Clostridium cellulolyticum on a chemically defined medium. Appl Environ Microbiol 67: 3837-3845.
  • Eroglu E, Gunduz U, Yucel M, Turker L, Eroglu, I (2004) Photobiological hydrogen production by using olive mill wastewater as a sole substrate source. Int J Hydrogen Energy 29: 163-171.
  • Ghirardi ML, Dubini A, Yu J, Maness PC (2009) Photobiological hydrogen-producing systems. Chem Soc Rev 38: 52-61.
  • Givry S, Prevot V, Duchiron F (2008) Lactic acid production from hemicellulosic hydrolyzate by cells of Lactobacillus bifermentans immobilized in Ca-alginate using response surface methodology. World J Microbiol Biotechnol 24: 745-752.
  • Guo W, Jia W, Li Y, Chen S (2010) Performances of Lactobacillus brevis for producing lactic acid from hydrolysate of lignocellulosics. Appl Biochem Biotechnol 161: 124-136.
  • Kars G, Gunduz U, Yucel M, Rakhely G, Kovacs K, Eroglu I (2009) Evaluation of hydrogen production by Rhodobacter sphaeroides O.U.001 and its hupSL deficient mutant using acetate and malate as carbon sources. Int J Hydrogen Energy 34: 2184-2190.
  • Kars G, Gunduz, U (2010) Towards a super H2 producer: Improvements in photofermentative biohydrogen production by genetic manipulations. Int J Hydrogen Energy 35: 664-656.
  • Kim JH, Block DE, Mills DA (2010) Simultaneous consumption of pentose and hexose sugars: an optimal microbial phenotype for efficient fermentation of lignocellulosic biomass. Appl Microbiol Biotechnol 88: 1077-1085.
  • Koku H, Eroglu I, Gunduz U, Yucel M, Turker L (2002) Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides. Int J Hydrogen Energy 27: 1315-1329.
  • Lo YC, Bai MD, Chen WM, Chang JS (2008) Cellulosic hydrogen production with a sequencing bacterial hydrolysis and dark fermentation strategy. Bioresource Technol 99: 8299-8303.
  • Lo YC, Su YC, Chen CY, Chen WM, Lee KS, Chang JS (2009a) Biohydrogen production from cellulosic hydrolysate produced via temperature-shift-enhanced bacterial cellulose hydrolysis. Bioresource Technol 100: 5802-5807.
  • Lo YC, Saratale GD, Chen WM, Baic MD, Chang JS (2009b) Isolation of cellulosehydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production. Enzyme Microb Technol 44: 417-425.
  • Odom JM, Wall JD (1983) Photoproduction of H2 from cellulose by an anaerobic bacterial coculture. Appl Environ Microbiol 45: 1300-1305.
  • Olson DG, McBride JE, Shaw AJ, Lynd LR (2012) Recent progress in consolidated bioprocessing. Curr Opin Biotech 23: 396-405.
  • Ren YL, Xing XH, Zhang Ch, Gou ZX (2005) A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes. Biotechnol Lett 27: 1029-1033.
  • Saratale GD, Chen SD, Lo YC, Saratale RG, Chang JS (2008) Outlook of biohydrogen production from lignocellulosic feedstock using dark fermentation - a review. J Sci Ind Res 67: 962-979.
  • Uyar B, Eroglu I, Yucel M, Gunduz U (2009) Photofermentative hydrogen production from volatile fatty acids present in dark fermentation effluents. Int J Hydrogen Energy 34: 4517-4523.
  • Wang A, Ren N, Shi Y, Lee DJ (2008) Bioaugmented hydrogen production from microcrystalline cellulose using co-culture Clostridium acetobutylicum X9 and Ethanoigenens harbinense B49. Int J Hydrogen Energy 33: 912-917.
  • Zhang YHP, Hong J, Ye Z (2009) Cellulase assays. In Biofuels. Methods and protocols. Mielenz RJ, ed, pp 213-232, Humana Press.
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