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2015 | 62 | 1 | 35-40
Article title

A novel laccase from fresh fruiting bodies of the wild medicinal mushroom Tricholoma matsutake

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EN
Abstracts
EN
The knowledge about biological activities of constituents from medicinal mushrooms belonging to the genus Tricholoma is limited. A 59-kDa laccase has now been purified from fresh fruiting bodies of the mushroom Tricholoma matsutake. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, affinity chromatography on ConA-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Of the various affinity and ion exchange chromatographic media employed, the laccase bound only on Con A-Sepharose. The activity of the laccase did not undergo major changes over the temperature range 20-80°C. However, all activity vanished following exposure to 100°C for 10 minutes. The enzyme activity varied only slightly over the pH range 3-5, with the optimal pH of 5, but exhibited a precipitous decline when the pH was increased to 6, and was undetectable at pH 8 and 9. The laccase showed activity in the decolorization of azo dyes without a mediator. Its N-terminal sequence demonstrated only slight resemblance to those of other mushroom laccases. The newly described laccase is distinctive from the previously isolated Tricholoma mushroom laccases in a number of aspects.
Year
Volume
62
Issue
1
Pages
35-40
Physical description
Dates
published
2015
received
2013-12-26
revised
2014-05-19
accepted
2015-01-09
online
2015-03-18
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p35kz
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