PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 62 | 2 | 323-328
Article title

Diverse expression of N-acetylglucosaminyltransferase V and complex-type β1,6-branched N-glycans in uveal and cutaneous melanoma cells

Content
Title variants
Languages of publication
EN
Abstracts
EN
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes occur principally in β1,4-N-acetylglucosaminyltransferase III (GnT-III) that catalyzes the synthesis of glycans with bisected N-acetylglucosamine (GlcNAc) and β1,6-N-acetylglucosaminyltransferase V (GnT-V) that is involved in forming β1,6-branched antenna in complex-type glycans. We searched for the reasons of a different behavior of CM and UM cells in the expression of GnT-III and GnT-V and their oligosaccharide products. Our study showed that UM cells have more β1,6-branched glycans than CM cells, what results from a higher expression of MGAT5 gene encoding GnT-V. The higher β1,6-branching of glycans in UM may contribute to their higher potential to migrate on fibronectin and weaker binding to main extracellular matrix proteins, observed in our previous studies.
Year
Volume
62
Issue
2
Pages
323-328
Physical description
Dates
published
2015
received
2015-05-07
revised
2015-05-25
accepted
2015-06-19
(unknown)
2015-06-22
References
  • Belmar-Lopez C, Mancheno-Corvo P, Saornil MA, Baril P, Vassaux G, Quintanilla M, Martin-Duque P (2008) Uveal vs. cutaneous melanoma. Origins and causes of the differences. Clin Transl Oncol 10: 137-142.
  • Bubka M, Link-Lenczowski P, Janik M, Pocheć E, Lityńska A (2014) Overexpression of N-acetylglucosaminyltransferases III and V in human melanoma cells. Implications for MCAM N-glycosylation. Biochimie 103: 37-49.
  • Christiansen MN, Chik J, Lee L, Anugraham M, Abrahams JL, Packer NH (2014) Cell surface protein glycosylation in cancer. Proteomics 14: 525-546.
  • Chudnovsky Y, Khavari PA, Adams AE (2005) Melanoma genetics and the development of rational therapeutics. J Clin Invest 115: 813-824.
  • Couldrey C, Green JE (2000) Metastases: the glycan connection. Breast Cancer Res 2: 321-323.
  • Dennis JW, Laferté S, Waghorne C, Breitman ML, Kerbel RS (1987) Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis. Science 236: 582-585.
  • Demetriou M, Nabi IR, Coppolino M, Dedhar S, Dennis JW (1995) Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V. J Cell Biol 130: 383-392.
  • Dosaka-Akita H, Miyoshi E, Suzuki O, Itoh T, Katoh H, Taniguchi N (2004) Expression of N-acetylglucosaminyltransferase V is associated with prognosis and histology in non-small cell lung cancers. Clin Cancer Res 10: 1773-1779.
  • Folberg R (1993) Tumor progression in ocular melanomas. J Invest Dermatol 100: 326S-331S.
  • Gu J, Isaji T, Xu Q, Kariya Y, Gu W, Fukuda T, Du Y (2012) Potential roles of N-glycosylation in cell adhesion. Glycoconj J 29: 599-607.
  • Gu J, Sato Y, Kariya Y, Isaji T, Taniguchi N, Fukuda T (2009) A mutual regulation between cell-cell adhesion and N-glycosylation: implication of the bisecting GlcNAc for biological functions. J Proteome Res 8: 431-435.
  • Guo HB, Zhang Y, Chen HL (2001) Relationship between metastasis-associated phenotypes and N-glycan structure of surface glycoproteins in human hepatocarcinoma cells. J Cancer Res Clin Oncol 127: 231-236.
  • Huang B, Wu Q, Ge Y, Zhang J, Sun L, Zhang Y, Fu L, Fan J, Wang Z (2014) Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis. Int J Oncol 44: 849-857.
  • Inamori K, Gu J, Ohira M, Kawasaki A, Nakamura Y, Nakagawa T, Kondo A, Miyoshi E, Nakagawara A, Taniguchi N (2006) High expression of N-acetylglucosaminyltransferase V in favorable neuroblastomas: Involvement of its effect on apoptosis. FEBS Lett 580: 627-632.
  • Isaji T, Kariya Y, Xu Q, Fukuda T, Taniguchi N, Gu J (2010) Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion. Methods Enzymol 480: 445-459.
  • Ishibashi K, Nishikawa A, Hayashi N, Kasahara A, Sato N, Fujii S, Kamada T, Taniguchi N (1989) N-acetylglucosaminyltransferase III in human serum, and liver and hepatoma tissues: increased activity in liver cirrhosis and hepatoma patients. Clin Chim Acta 185: 325-332.
  • Ishimura H, Takahashi T, Nakagawa H, Nishimura S, Arai Y, Horikawa Y, Habuchi T, Miyoshi E, Kyan A, Hagisawa S, Ohyama C (2006) N-acetylglucosaminyltransferase V and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer. Clin Cancer Res 12: 2506-2511.
  • Ito Y, Miyoshi E, Sakon M, Takeda T, Noda K, Tsujimoto M, Ito S, Honda H, Takemura F, Wakasa K, Monden M, Matsuura N, Taniguchi N (2001) Elevated expression of UDP-N-acetylglucosamine: alphamannoside beta1,6-N-acetylglucosaminyltransferase is an early event in hepatocarcinogenesis. Int J Cancer 91: 631-637.
  • Janik ME, Lityńska A, Przybyło M (2014) Studies on primary uveal and cutaneous melanoma cell interaction with vitronectin. Cell Biol Int 38: 942-952.
  • Janik ME, Przybyło M, Pocheć E, Pokrywka M, Lityńska A (2010) Effect of alpha3beta1 and alpha(v)beta3 integrin glycosylation on interaction of melanoma cells with vitronectin. Acta Biochim Pol 57: 55-61.
  • Jovanovic P, Mihajlovic M, Djordjevic-Jocic J, Vlajkovic S, Cekic S, Stefanovic V (2013) Ocular melanoma: an overview of the current status. Int J Clin Exp Pathol 6: 1230-1244.
  • Karaçali S, Izzetoğlu S, Deveci R (2014) Glycosylation changes leading to the increase in size on the common core of N-glycans, required enzymes, and related cancer-associated proteins Turk J Biol 38: 754-771.
  • Kim YJ, Varki A (1997) Perspectives on the significance of altered glycosylation of glycoproteins in cancer. Glycoconj J 14: 569-576.
  • Kremser ME, Przybyło M, Hoja-Łukowicz D, Pocheć E, Amoresano A, Carpentieri A, Bubka M, Lityńska A (2008) Characterisation of alpha3beta1 and alpha(v)beta3 integrin N-oligosaccharides in metastatic melanoma WM9 and WM239 cell lines. Biochim Biophys Acta 1780: 1421-1431.
  • Kyan A, Kamimura N, Hagisawa S, Hatakeyama S, Koie T, Yoneyama T, Arai Y, Nakagawa H, Nishimura S, Miyoshi E, Hashimoto Y, Ohyama C (2008) Positive expressions of N-acetylglucosaminyltransferase-V (GnT-V) and beta1-6 branching N-linked oligosaccharides in human testicular germ cells diminish during malignant transformation and progression. Int J Oncol 32: 129-134.
  • Laidler P, Lityńska A (1997) Tumor cell N-glycans in metastasis. Acta Biochim Pol 44: 343-357.
  • Laidler P, Lityńska A, Hoja-Łukowicz D, Łabedz M, Przybyło M, Ciołczyk-Wierzbicka D, Pocheć E, Trebacz E, Kremser E (2006) Characterization of glycosylation and adherent properties of melanoma cell lines. Cancer Immunol Immunother 55: 112-118.
  • Lityńska A, Przybyło M, Hoja-Łukowicz D, Pochec E, Kremser M, Ciołczyk-Wierzbicka D, Łabędź M, Laidler P (2008) Protein glycosylation as marker of melanoma progression. Curr Cancer Ther Rev 4: 144-148.
  • Miyoshi E, Terao M, Kamada Y (2012) Physiological roles of N-acetylglucosaminyltransferase V (GnT-V) in mice. BMB Rep 45: 554-559.
  • Murata K, Miyoshi E, Kameyama M, Ishikawa O, Kabuto T, Sasaki Y, Hiratsuka M, Ohigashi H, Ishiguro S, Ito S, Honda H, Takemura F, Taniguchi N, Imaoka S (2000) Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis. Clin Cancer Res 6: 1772-1777.
  • Narasimhan S, Schachter H, Rajalakshmi S (1988) Expression of N-acetylglucosaminyltransferase III in hepatic nodules during rat liver carcinogenesis promoted by orotic acid. J Biol Chem 263: 1273-1281.
  • Nikolaou VA, Stratigos AJ, Flaherty KT, Tsao H (2012) Melanoma: new insights and new therapies. J Invest Dermatol 132: 854-863.
  • Pocheć E, Bubka M, Rydlewska M, Janik M, Pokrywka M, Lityńska A (2015) Aberrant glycosylation of αvβ3 integrin is associated with melanoma progression. Anticancer Res 35: 2093-2103.
  • Pocheć E, Janik M, Hoja-Łukowicz D, Link-Lenczowski P, Przybyło M, Lityńska A (2013) Expression of integrins α3β1 and α5β1 and GlcNAc β1,6 glycan branching influences metastatic melanoma cell migration on fibronectin. Eur J Cell Biol 92: 355-362.
  • Pocheć E, Lityńska A, Amoresano A, Casbarra A (2003) Glycosylation profile of integrin alpha 3 beta 1 changes with melanoma progression. Biochim Biophys Acta 1643: 113-123.
  • Przybyło M, Martuszewska D, Pocheć E, Hoja-Łukowicz D, Lityńska A (2007) Identification of proteins bearing beta1-6 branched N-glycans in human melanoma cell lines from different progression stages by tandem mass spectrometry analysis. Biochim Biophys Acta 1770: 1427-1435.
  • Przybyło M, Pocheć E, Link-Lenczowski P, Lityńska A (2008) Beta1-6 branching of cell surface glycoproteins may contribute to uveal melanoma progression by up-regulating cell motility. Mol Vis 14: 625-636.
  • Seftor RE, Seftor EA, Hendrix MJ (1999) Molecular role(s) for integrins in human melanoma invasion. Cancer Metastasis Rev 18: 359-375.
  • Seto K, Uchida F, Baba O, Yamatoji M, Karube R, Warabi E, Sakai S, Hasegawa S, Yamagata K, Yanagawa T, Onizawa K, Miyoshi E, Shoda J, Bukawa H (2013) Negative expression of N-acetylglucosaminyltransferase V in oral squamous cell carcinoma correlates with poor prognosis. Springerplus 2: 657.
  • Shtivelman E, Davies MQ, Hwu P, Yang J, Lotem M, Oren M, Flaherty KT, Fisher DE (2014) Pathways and therapeutic targets in melanoma. Oncotarget 5: 1701-1752.
  • Takahashi N, Yamamoto E, Ino K, Miyoshi E, Nagasaka T, Kajiyama H, Shibata K, Nawa A, Kikkawa F (2009) High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary. Oncol Rep 22: 1027-1032.
  • Taniguchi N, Korekane H (2011) Branched N-glycans and their implications for cell adhesion, signaling and clinical applications for cancer biomarkers and in therapeutics. BMB Rep 44: 772-781.
  • Taniguchi N, Miyoshi E, Ko JH, Ikeda Y, Ihara Y (1999) Implication of N-acetylglucosaminyltransferases III and V in cancer: gene regulation and signaling mechanism. Biochim Biophys Acta 1455: 287-300.
  • Taniguchi N, Yoshimura M, Miyoshi E, Ihara Y, Nishikawa A, Fujii S (1996) Remodeling of cell surface glycoproteins by N-acetylglucosaminyltransferase III gene transfection: modulation of metastatic potentials and down regulation of hepatitis B virus replication. Glycobiology 6: 691-694.
  • ten Berge PJ, Danen EH, van Muijen GN, Jager MJ, Ruiter DJ (1993) Integrin expression in uveal melanoma differs from cutaneous melanoma. Invest Ophthalmol Vis Sci 34: 3635-3640.
  • Tian H, Miyoshi E, Kawaguchi N, Shaker M, Ito Y, Taniguchi N, Tsujimoto M, Matsuura N (2008) The implication of N-acetylglucosaminyltransferase V expression in gastric cancer. Pathobiology 75: 288-294.
  • van den Bosch T, Kilic E, Paridaens D, de Klein A (2010) Genetics of uveal melanoma and cutaneous melanoma: two of a kind? Dermatol Res Pract 2010: 360136.
  • Yamamoto E, Ino K, Miyoshi E, Shibata K, Takahashi N, Kajiyama H, Nawa A, Nomura S, Nagasaka T, Kikkawa F (2007) Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis. Br J Cancer 97: 1538-1544.
  • Yoshimura M, Nishikawa A, Ihara Y, Nishiura T, Nakao H, Kanayama Y, Matuzawa Y, Taniguchi N (1995a) High expression of UDP-N-acetylglucosamine: beta-D mannoside beta-1,4-N-acetylglucosaminyltransferase III (GnT-III) in chronic myelogenous leukemia in blast crisis. Int J Cancer 60: 443-449.
  • Yoshimura M, Nishikawa A, Ihara Y, Taniguchi S, Taniguchi N (1995b) Suppression of lung metastasis of B16 mouse melanoma by N-acetylglucosaminyltransferase III gene transfection. Proc Natl Acad Sci U S A 92: 8754-8758.
  • Zhang Y, Zhao JH, Zhang XY, Guo HB, Liu F, Chen HL (2004) Relations of the type and branch of surface N-glycans to cell adhesion, migration and integrin expressions. Mol Cell Biochem 260: 137-146.
  • Zhao Y, Nakagawa T, Itoh S, Inamori K, Isaji T, Kariya Y, Kondo A, Miyoshi E, Miyazaki K, Kawasaki N, Taniguchi N, Gu J (2006) N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha3beta1 integrin-mediated cell migration. J Biol Chem 281: 32122-32130.
  • Zhao YY, Takahashi M, Gu JG, Miyoshi E, Matsumoto A, Kitazume S, Taniguchi N (2008) Functional roles of N-glycans in cell signaling and cell adhesion in cancer. Cancer Sci 99: 1304-1310.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p323kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.