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2015 | 62 | 2 | 311-316
Article title

Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold

Content
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Languages of publication
EN
Abstracts
EN
Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.
Publisher

Year
Volume
62
Issue
2
Pages
311-316
Physical description
Dates
published
2015
received
2015-03-12
revised
2015-05-02
accepted
2015-05-06
(unknown)
2015-05-18
Contributors
  • Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
author
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • Division of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland
  • Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
  • Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznań, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p311kz
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