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2015 | 62 | 2 | 281-286
Article title

Quantification of 5-methyl-2'-deoxycytidine in the DNA

Content
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EN
Abstracts
EN
Methylation at position 5 of cytosine (Cyt) at the CpG sequences leading to formation of 5-methyl-cytosine (m5Cyt) is an important element of epigenetic regulation of gene expression. Modification of the normal methylation pattern, unique to each organism, leads to the development of pathological processes and diseases, including cancer. Therefore, quantification of the DNA methylation and analysis of changes in the methylation pattern is very important from a practical point of view and can be used for diagnostic purposes, as well as monitoring of the treatment progress. In this paper we present a new method for quantification of 5-methyl-2'deoxycytidine (m5C) in the DNA. The technique is based on conversion of m5C into fluorescent 3,N4-etheno-5-methyl-2'deoxycytidine (εm5C) and its identification by reversed-phase high-performance liquid chromatography (RP-HPLC). The assay was used to evaluate m5C concentration in DNA of calf thymus and peripheral blood of cows bred under different conditions. This approach can be applied for measuring of 5-methylcytosine in cellular DNA from different cells and tissues.
Year
Volume
62
Issue
2
Pages
281-286
Physical description
Dates
published
2015
received
2015-02-09
revised
2015-04-29
accepted
2015-05-20
(unknown)
2015-06-22
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p281kz
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