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2015 | 62 | 2 | 273-280
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Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds

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The current knowledge and documentation on the origins and relationship between Gyimesi Racka reared in Hungary and the Romanian Turcana is rather controversial. Lack of information and scientific reliable proofs for the divergent theories found in the two countries motivated us to implement a trial using molecular methods to assess the genetic distance and diversity in the two breeds. Hair follicles were collected from Gyimesi Racka (2 phenotypes) and from Turcana (6 ecotypes). The 599 bp segment of the D-loop region of the mitochondrial DNA was sequenced. Altogether, 42 haplotypes were identified, while 23 were found in both populations. Populations were highly diverse according to the haplotype and nucleotide diversity indices. AMOVA analysis showed that most of the variation was observed within populations (98%), indicating a weak genetic structure between the two breeds. Animals were grouped into seven groups based on their phenotype; however genetic distances among them were also low. Tajima's D, Fu's Fs, goodness-of-fit statistics, mismatch distribution and network analysis suggested recent demographic expansion. Current comprehensive mtDNA study indicates that there is very low level of genetic differentiation between the Gyimesi Racka and Turcana populations therefore they are de facto one trans-boundary breed.
Physical description
  • Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary
  • Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary
  • Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary
  • Department of Animal Production Engineering, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania', Timisoara, 300645, Romania
  • Department of Animal Production Engineering, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania', Timisoara, 300645, Romania
  • Research and Development Station for Sheep and Goats, Caransebes, 325400, Romania
  • Árnyasi M, Komlósi I, Kent MP, Czeglédi L, Gulyás G, Jávor A (2013) Investigation of polymorphisms and association of the ABCG2 gene with milk production traits in sheep. Livest Sci 154: 64-68.
  • Bandelt HJ, Forster P, Rohl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16: 37-48.
  • Cinkulov M, Tapio M, Ozerov M, Kiselyova T, Marzanov N, Pihler I, Olsaker I, Vegara M, Kantanen J (2008) Genetic differentiation between the Old and New types of Serbian Tsigai sheep. Genet Sel Evol 40: 321-331.
  • Cinkulov M, Popovski Z, Porcu K, Tanaskovska B, Hodzic A, Bytyqi H, Mehmeti H, Margeta V, Djedovic R, Hoda A, Trailovic R, Brka M, Markovic B, Vazic B, Vegara M, Olsaker I, Kantanen J (2008) Genetic diversity and structure of the West Balkan Pramenka sheep types as revealed by microsatellite and mitochondrial DNA analysis. J Anim Breed Genet 125: 417-426.
  • Draganescu C, Grosu H (2010) Valachian (Zackel) heritage philetic sheep group - a taxonomic problem. Sci Pap Rom Acad 1: 1-8.
  • Excoffier L, Schneider S (1999) Why hunter-gatherer populations do not show signs of Pleistocene demographic expansions. Proc Nat Acad Sci USA 96: 10597-10602.
  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 1: 47-50.
  • FAO/IAEA Agriculture Biotechnology Laboratory (2005) Handbook of Laboratory Exercises, IAEA Laboratories. Seibersdorf, Austria.
  • Ilisiu E, Daraban S, Neascu G, Ilisiu V, Rahman G (2010) Improvement of lamb production in Romania by crossbreeding of local Tsigai breed with high performance breeds. Landbauf vTI Agric Forest Res 60: 259-266.
  • Juler C (2014) Dupa coada oilor: long-distance transhumance and its survival in Romania. Pastoral Res Pol Pract 4: 1-17.
  • Kawecka A, Piorkowska K (2011) Characteristics of the genetic structure of native sheep breeds. Ann Anim Sci 11: 371-382.
  • Kawecka A, Krupinski J (2014) Sheep in the Polish Carpathians: Genetic resources conservation of the Podhale Zackel and Coloured Mountain sheep. Geomat Landman Lans 1: 35-45.
  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111-120.
  • Kusza Sz, Nagy I, Sasvari Z, Stagel A, Nemeth T, Molnar A, Kume K, Bosze Z, Javor A, Kukovics S (2008) Genetic diversity and population structure of Tsigai and Zackel type of sheep breeds in the Central-, Eastern- and Southern-European regions. Small Rumin Res 78: 13-23.
  • Lancioni H, Di Lorenzo P, Ceccobelli S, Perego UA, Miglio A (2013) Phylogenetic relationships of three italian merino-derived sheep breeds evaluated through a complete mitogenome analysis. PLoS ONE 8: e73712.
  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.
  • Lawson Handley LJ, Byrne K, Santucci F, Townsend S, Taylor M, Bruford MW, Hewitt GM (2007) Genetic structure of European sheep breeds. Heredity 99: 620-631.
  • Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.
  • Ligda C, Altarayrah J, Georgoudis A, ECONOGENE Consortium (2009) Genetic analysis of Greek sheep breeds using microsatellite markers for setting conservation priorities. Small Rumin Res 83: 42-48.
  • Padeanu I (2010) Productia de carne la ovine. Mirton Press, Timisoara, Romania (in what language?).
  • Peter C, Bruford M, Perez T, Dalamitra S, Hewitt G, Erhardt G, ECONOGENE Consortium (2007) Genetic diversity and subdivision of 57 European and Middle-Eastern sheep breeds. Anim Genet 38: 37-44.
  • Petrovic MM, Aleksic S, Petrovic MP, Petrovic M, Pantelic V, Novakovic Z, Ruzic-Muslic D (2013) Potentials of Serbian livestock production - outlook and future. Biotech Anim Husb 29: 1-17.
  • Posada D, Crandall KA (2001) Intraspecific gene genealogies: trees grafting into networks Trends Ecol Evol 16: 37-45.
  • Rege JEO, Gibson JP (2003) Animal genetic resources and economic development: issues in relation to economic valuation. Ecol Econ 45: 319-330.
  • Rogers AR, Harpending HC (1992) Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 9: 552-569.
  • Savic M, Trailovic R, Petrujkic B, Beckei Z, Dimitrijevic B, Dimitrijevic V (2013) Determining the value of Vlashko-Vitoroga Zackel sheep for the conservation process. Acta Vet Beogr 63: 621-629.
  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30: 2725-2729.
  • Tapio M, Ozerov M, Tapio I, Toro MA, Marzanov N, Cinkulov M, Goncharenko G, Kiselyova T, Murawski M, Kantanen J (2010) Microsatellite-based genetic diversity and population structure of domestic sheep in northern Eurasia. BMC Genetics 11: 76.
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