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2015 | 62 | 2 | 215-219
Article title

A compound C-terminal nuclear localization signal of human SA2 stromalin

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Languages of publication
EN
Abstracts
EN
Stromalins are evolutionarily conserved multifunctional proteins with the best known function in sister chromatid cohesion. Human SA2 stromalin, likely involved in the establishment of cohesion, contains numerous potential nuclear localization (NLS) and nuclear export signals (NES). Previously we have found that the C-terminus of SA2 contains NLS(s) functional in human cells. However, the identity of this signal remained unclear since three NLS-like sequences are present in that region. Here we analyzed the functionality of these putative signals by expressing GFP-tagged C-terminal part of SA2 or its fragments in a human cell line and in the yeast Saccharomyces cerevisiae. We found that in human cells the nuclear import is dependent on a unique compound di- or tripartite signal containing unusually long linkers between clusters of basic amino acids. Upon expression of the same SA2 fragment in yeast this signal is also functional and can be easily studied in more detail.
Publisher

Year
Volume
62
Issue
2
Pages
215-219
Physical description
Dates
published
2015
received
2014-10-10
revised
2015-03-12
accepted
2015-05-05
(unknown)
2015-05-15
Contributors
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Department of Medical Genetics, Institute of Mother and Child, Warsaw, Poland
author
  • Institute of Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
References
  • Fontes MR, Teh T, Kobe B (2000) Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha. J Mol Biol 297: 1183-1194.
  • Gandhi R, Gillespie PJ, Hirano T (2006) Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Curr Biol 16: 2406-2417.
  • Gould CM, Diella F, Via A, Puntervoll P, Gemund C et al. (2009) ELM: the status of the 2010 eukaryotic linear motif resource. Nucleic Acids Res 38: (Database issue) D167-D180.
  • Horton P, Nakai K (1997) Better prediction of protein cellular localization sites with the nearest neighbors classifier. Proc Int Conf Intell Syst Mol Biol 5: 147-152.
  • Haering CH, Lowe J, Hochwagen A, Nasmyth K (2002) Molecular architecture of SMC proteins and the yeast cohesin complex. Mol Cell 9: 773-788.
  • Hauf S, Roitinger E, Koch B, Dittrich CM, Mechtler K, Peters JM (2005) Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2. PLoS Biol 3: e69.
  • Kalderon D, Richardson WD, Markham AF, Smith AE (1984a) Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311: 33-38.
  • Kalderon D, Roberts BL, Richardson WD, Smith AE (1984b) A short amino acid sequence able to specify nuclear location. Cell 39: 499-509.
  • Kong X, Ball AR Jr, Pham HX, Zeng W, Chen HY, Schmiesing JA, Kim JS, Berns M, Yokomori K (2014) Distinct functions of human cohesin-SA1 and cohesin-SA2 in double-strand break repair. Mol Cell Biol 34: 685-698.
  • Lange A, McLane LM, Mills RE, Devine SE, Corbett AH (2010) Expanding the definition of the classical bipartite nuclear localization signal. Traffic 11: 311-323.
  • Losada A, Yokochi T, Kobayashi R, Hirano T (2000) Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes. J Cell Biol 150: 405-416.
  • Losada A (2014) Cohesin in cancer: chromosome segregation and beyond. Nat Rev Cancer 14: 389-393.
  • Marfori M, Mynott A, Ellis JJ, Mehdi AM, Saunders NF, Curmi PM, Forwood JK, Bodén M, Kobe B (2011) Molecular basis for specificity of nuclear import and prediction of nuclear localization. Biochim Biophys Acta 1813: 1562-1577.
  • Marston AL (2014) Chromosome segregation in budding yeast: sister chromatid cohesion and related mechanisms. Genetics 196: 31-63.
  • Murayama Y, Uhlmann F (2014) Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 505: 367-371.
  • Nasmyth K, Haering CH (2009) Cohesin: its roles and mechanisms. Annu Rev Genet 43: 525-558.
  • Neville M, Rosbash M (1999) The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae. EMBO J 18: 3746-3756.
  • Sherman F (1991) Getting started with yeast. Methods Enzymol 194: 3-21.
  • Sumara I, Vorlaufer E, Gieffers C, Peters BH, Peters JM (2000) Characterization of vertebrate cohesin complexes and their regulation in prophase. J Cell Biol 151: 749-761.
  • Sutani T, Kawaguchi T, Kanno R, Itoh T, Shirahige K (2009) Budding yeast Wpl1(Rad61)-Pds5 complex counteracts sister chromatid cohesion-establishing reaction. Curr Biol 19: 492-497.
  • Tarnowski LJ, Kowalec P, Milewski M, Jurek M, Plochocka D, Fronk J, Kurlandzka A (2012) Nuclear import and export signals of human cohesins SA1/STAG1 and SA2/STAG2 expressed in Saccharomyces cerevisiae. PLoS One 7: e38740.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p215kz
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