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2015 | 62 | 2 | 207-213
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Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver

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Quercetin, a member of the flavonoid family is a major antioxidant acquired in humans by food consumption, while Cadmium fluoride (CdF2) is one of the naturally occurring chemicals having adverse effects. The protective effect of quercetin on time dependent oxidative damage induced in mice liver by CdF2 was studied in the following groups of mice consisting of six mice each: (i) control group; (ii) mice treated with single i.p injection of 2 mg/kg bw CdF2 for 24 h; (iii) mice treated with single i.p injection of 2 mg/kg bw CdF2 for 48 h; (iv) mice treated with single i.p injection of quercetin (100 mg/kg bw); (v) mice treated with i.p injection of 100 mg/kg bw of quercetin followed by i.p injection of CdF2 (2 mg/kg bw) for 24 h; and (vi) mice treated with i.p injection of 100mg/kg bw of quercetin followed by CdF2 (2 mg/kg bw) for 48 h. Administration of quercetin two hours before CdF2 significantly reduced the biochemical alterations in reduced glutathione, ascorbic acid, lipid peroxidation, super oxide dismutase, catalase and total protein (p<0.05). Histopathology also showed the protective effect of quercetin. The livers treated with CdF2 were atrophic, markedly nodular, inflamed and necrotic. However, this effect was reduced to a minimum in the mice pre-treated for two hours with quercetin.
Physical description
  • Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
  • Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
  • Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
  • Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
  • Adachi K, Dote T, Dote E, Mitsui G, Kono K (2007) Strong acute toxicity, severe hepatic damage, renal injury and abnormal serum electrolytes after intravenous administration of cadmium fluoride in rats. J Occup Health 49: 235-241.
  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105: 121-126.
  • Asagba SO, Adaikpoh M, Kadiri H, Obi FO (2007) Influence of aqueous extract of Hibiscus sabdariffa L. petalon cadmium toxicity in rats. Biol Trace Elem Res 115: 47-57.
  • Bánhegyi G, Csala M, Braun L, Garzó T, Mandl J (1996) Ascorbate synthesis-dependent glutathione consumption in mouse liver. FEBS Lett 381: 39-41.
  • Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882-888.
  • Borm PJ, Schins RP, Albrecht C (2004) Inhaled particles and lung cancer, Part B: Paradigms and risk assessment. Int J Cancer 110: 3-14.
  • Braun L, Csala M, Poussu A, Garzó T, Mandl J, Bánhegyi G (1996) Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes. FEBS Lett 388: 173-176.
  • Choi YJ, Kang JS, Park JH, Lee YJ, Choi JS, Kang YH. (2003) Polyphenolic flavonoids differ in their antiapoptotic efficacy in hydrogen peroxide treated human vascular endothelial cells. J Nutr 133: 985-991.
  • Christen S, Thomas SR, Garner B, Stocker R (1994) Inhibition by interferon-gamma of human mononuclear cell-mediated low density lipoprotein oxidation. Participation of tryptophan metabolism along the kynurenine pathway. J Clin Invest 93: 2149-2158.
  • Ellis JK, Athersuch TJ, Thomas LD, Teichert F, Pérez-Trujillo M, Svendsen C, Spurgeon DJ, Singh R, Järup L, Bundy JG, Keun HC (2012) Metabolic profiling detects early effects of environmental and lifestyle exposure to cadmium in a human population. BMC Med 10: 61-70.
  • Ferrali M, Signorini C, Ciccoli L, Bambagioni S, Rossi V, Pompella A, Comporti M (2000) Protection of erythrocytes against oxidative damage and autologous immunoglobulin G (IgG) binding by iron chelatorfluor-benzoil-pyridoxalhydrazone. Biochem Pharmacol 59: 1365-1373.
  • Jagota SK, Dani HM (1982) A new colorimetric technique for the estimation of vitamin C using folin-phenol reagent. Anal Biochem 127: 178-182.
  • Klaassen CD, Liu J, Choudhuri S (1999) Metallothionein: an intracellular protein to protect against cadmium toxicity. Annu Rev Pharmacol Toxicol 39: 267-297.
  • Knaapen AM, Borm PJ, Albrecht C, Schins RP (2004) Inhaled particles and lung cancer. Part A: Mechanisms. Int J Cancer 109: 799-809.
  • Lushchak VI (2012) Glutathione homeostasis and functions: potential targets for medical interventions. J Amino Acids 12: 1-26.
  • Markwell MA, Haas SM, Bieber LL, Tolbert NE (1978) A modification of the Lowry Procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87: 206-210.
  • Mãrtensson J, Meister A (1992) Glutathione deficiency increases hepatic ascorbic acid synthesis in adult mice. Proc Natl Acad Sci USA 89: 11566-11568.
  • Morales AI, Vicente-Sanchez C, Sandoval JM, Egido J, Mayoral P, Arévalo MA, Fernández-Tagarro M, López-Novoa JM, Pérez-Barriocanal F (2006) Protective effect of quercetin on experimental chronic cadmium nephrotoxicity in rats is based on its antioxidant properties. Food Chem Toxicol 44: 2092-2100.
  • Siddiqi NJ, Zargar S (2014) Effect of quercetin on cadmium fluoride induced alterations in hydroxyproline/collagen content in mice liver. Connective Tissue Research 55: 234-238.
  • Nishikimi M, Appaji N, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazinemethosulfate and molecular oxygen. Biochem Biophys Res Commun 46: 849-854.
  • Nordberg GF (1984) Chelating agents and cadmium toxicity: problems and prospects. Environ Health Perspect 54: 213-218.
  • Pavanato A, Tuñón MJ, Sánchez-Campos S, Marroni CA, Llesuy S, González-Gallego J, Marroni N (2003) Effects of quercetin on liver damage in rats with carbon tetrachloride-induced cirrhosis. Dig Dis Sci 48: 824-829.
  • Policard A, Collet A (1953) Recherches experimentales sur la nocivité des poussières de spathfluor (fluorine). Arch Mal Prof 14: 117-122.
  • Rashid K, Sinha K, Sil PC (2013) An update on oxidative stress-mediated organ pathophysiology. Food Chem Toxicol 62: 584-600.
  • Renugadevi J, Prabu SM (2009) Naringenin protects against cadmium induced oxidative renal dysfunction in rats. Toxicology 256: 128-134.
  • Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20: 933-956.
  • Zargar S, Siddiqi NJ, Khan TH, Elredah IE (2014) Effect of cadmium fluoride and quercetin on in vivo activity of indoleamine 2,3-dioxygenase in mice liver and kidney. Fluoride 47: 31-42.
  • Schins RP (2002) Mechanisms of genotoxicity of particles and fibers. Inhal Toxicol 14: 57-78.
  • Soni MG, Kachole MS, Pawar SS (1984) Alterations in drug metabolising enzymes and lipid peroxidation in different rat tissues by fluoride. Toxicol Lett 21: 167-72.
  • Utley HG, Berheim F, Hochstein P (1967) Effect of sulfhydryl reagent on peroxiation in microsomes. Arch Biochem Biophys 118: 29-32.
  • Van Maanen JM, Borm PJ, Knaapen A, van Herwijnen M, Schilderman PA, Smith KR, Aust AE, Tomatis M, Fubini B (1999) In vitro effects of coal fly ashes: Hydroxyl radical generation, iron release, and DNA damage and toxicity in rat lung epithelial cells. Inhal Toxicol 11: 1123-1141.
  • Wolfe RR (2001) Control of muscle protein breakdown: effects of activity and nutritional states. Int J Sport Nutr Exerc Metab 11 (Suppl): S164.
  • Zhong BZ, Whong WZ, Ong TM (1997) Detection of mineral-dust-induced DNA damage in two mammalian cell lines using the alkaline single cell gel/comet assay. Mutat Res 393: 181-187.
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