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2015 | 62 | 2 | 173-176
Article title

Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation

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EN
Abstracts
EN
Histone modifications are involved in the DNA damage response (DDR). Here, by utilizing an ELISA immunoassay we assessed the methylation at H3K9 (H3K9me2 and H3K9me3) in two cell lines with differential sensitivity to radiation-induced apoptosis, HeLa (sensitive) and MCF-7 (resistant). We found that DNA damage induction by γ-irradiation leads to considerable accumulation (up to 5-fold) of H3K9me2 and H3K9me3, but not of H4K20me3 (control modification) in MCF-7 cells (p<0.05). Interestingly, a lower dose (2 Gy) was more effective than 5 Gy. In HeLa cells a smaller effect (approx. 1.5-1.8-fold) was evident only at 5 Gy. In conclusion, our findings reveal that DNA damage leads to specific accumulation of H3K9me2 and H3K9me3 in a cell-type specific manner.
Publisher

Year
Volume
62
Issue
2
Pages
173-176
Physical description
Dates
published
2015
received
2014-05-14
revised
2015-03-10
accepted
2015-05-08
(unknown)
2015-05-26
Contributors
  • Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey
author
  • Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey
author
  • Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey
author
  • Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p173kz
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