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2015 | 62 | 1 | 161-166
Article title

The most widespread problems in the function-based microbial metagenomics

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EN
Abstracts
EN
Metagenomics is a powerful tool to better understand the microbial niches, especially these from extreme habitats like oceans and seas, hot springs or deserts. However, one who is going to face the metagenomic studies should realize the challenges which might occur in the course of experiments. This manuscript indicates common problems in function-driven metagenomics, especially factors that influence gene expression are taken into account. Codon usage bias, internal cell accumulation, correct protein folding or presence of proper initiation factors are discussed and possible ways to overcome these problems are proposed. Finally, the annotation process is described, including possible limitations that one should take under consideration. What is more, the most popular databases for metagenomic data are mentioned and discussed.
Publisher

Year
Volume
62
Issue
1
Pages
161-166
Physical description
Dates
published
2015
received
2014-10-10
revised
2014-10-27
accepted
2014-11-12
online
2015-02-13
Contributors
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
author
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
author
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p161kz
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