Sampling, metadata and DNA extraction - important steps in metagenomic studies

• Authors: Agnieszka Felczykowska , Anna Krajewska , Sylwia Zielińska , Joanna Łoś
• Languages of publication: EN

Abstracts

EN

Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.

Keywords

EN

sampling; DNA extraction; DNA purification; soil; water; wastewater; metadata

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 1
• Pages: 151-160

Dates

published

2015

received

2014-10-10

revised

2014-10-30

accepted

2014-11-12

online

2015-02-13

Contributors

author

Agnieszka Felczykowska

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

author

Anna Krajewska

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

author

Sylwia Zielińska

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

author

Joanna Łoś

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

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