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2015 | 62 | 1 | 119-122
Article title

Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification

Content
Title variants
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EN
Abstracts
EN
Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.
Year
Volume
62
Issue
1
Pages
119-122
Physical description
Dates
published
2015
received
2014-07-18
revised
2014-11-13
accepted
2014-11-21
online
2015-02-24
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv62p119kz
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