Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds

• Authors: Szilvia Kusza , Nikolett Sziszkosz , Krisztina Nagy , Amela Masala , Sándor Kukovics , Jávor András
• Languages of publication: EN

Abstracts

EN

Genetic polymorphism at the β-lactoglobulin (β-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of β-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the β-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the Rusty and the Zomborski Tsigai (59%, 60%). The C allele was found only in one individual from Serbian Cokanski flock. These results differ from those that refer to other native sheep breeds. In the Cokanski Tsigai, deviation from the Hardy-Weinberg equilibrium was detected. Genetic relationship based on β-lactoglobulin polymorphism was the closest between the Rusty and the Cokanski Tsigai among the studied populations and between sheep and goat among the other ruminants. Part of the promoter region (254 bp) of β-LG in studied sheep breeds were sequenced in order to identify polymorphisms, analyze haplotypes, and phylogenetic relationship among them. Sequencing analysis and alignment of the obtained sequences showed one haplotype. Analysis of more samples and longer parts of the promoter region of β-LG are needed to reconstruct a phylogenetic tree.

Keywords

EN

Tsigai group; Zackel sheep; β-LG; allele frequencies; genetic relationship; sequencing

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 1
• Pages: 109-112

Dates

published

2015

received

2014-06-25

revised

2014-11-12

accepted

2014-12-17

online

2015-03-02

Contributors

author

Szilvia Kusza

• University of Debrecen, Institute of Animal Science, Biotechnology and Nature Conservation, Hungary

author

Nikolett Sziszkosz

• University of Debrecen, Institute of Animal Science, Biotechnology and Nature Conservation, Hungary

author

Krisztina Nagy

• University of Debrecen, Institute of Animal Science, Biotechnology and Nature Conservation, Hungary

author

Amela Masala

• University Sarajevo, Department of Physiology, Bosnia and Herzegovina

author

Sándor Kukovics

• Research Institute for Animal Breeding and Nutrition, Hungary

author

Jávor András

• University of Debrecen, Institute of Animal Science, Biotechnology and Nature Conservation, Hungary

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