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2014 | 61 | 1 | 85-90
Article title

Determination of lysosomal exoglycosidases in human saliva

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EN
Abstracts
EN
Background: Currently we observe a growing interest in human saliva as a non-invasive material for diagnosis and monitoring of general and oral diseases. Methods: The aim of our study was adaptation of the Marciniak et al. (Marciniak J, Zalewska A, Popko J, Zwierz K, 2006, Clin Chem Lab Med 44: 933-937) method for determination of HEX and GLU activity in synovial fluid, and for determination of: HEX and GLU, as well as MAN, GAL, and FUC activity in human saliva. Results: Under optimal conditions, 10 μl of saliva for HEX, and 30 μl for GLU, MAN, GAL and FUC, were sufficient for determination of human salivary exoglycosidases activity with variation coefficient ranging from 0.89 for GLU to 0.99 for GAL. Conclusion: The adapted method for exoglycosidases activity determination in human saliva is sufficiently sensitive and precise to use in clinical diagnosis.
Publisher

Year
Volume
61
Issue
1
Pages
85-90
Physical description
Dates
published
2014
received
2013-09-08
revised
2014-01-20
accepted
2014-02-25
(unknown)
2014-03-18
Contributors
  • Medical Institute, College of Computer Science and Business Administration, Lomza, Poland
author
  • Department of Conservative Dentistry, Medical University in Bialystok, Poland
  • The Institute of Health Care, The Higher Vocational School, Suwalki, Poland
  • Department of Psychiatry, Medical University in Bialystok, Poland
  • Department of Conservative Dentistry, Medical University in Bialystok, Poland
  • Medical College of the Universal Education Society, Lomza, Poland
  • Medical College of the Universal Education Society, Lomza, Poland
References
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  • Marciniak J, Zalewska A, Popko J, Zwierz K (2006) Optimization of an enzymatic method for the determination of lysosomal N-acetyl-beta-D-hexosaminidase and beta-glucuronidase in synovial fluid. Clin Chem Lab Med 44: 933-937.
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  • Popko J, Marciniak J, Ilendo E, Knaś M, Guszczyn T, Stasiak-Barmuta A et al (2008) Profile of exoglycosidases in synovial cell cultures derived from human synovial membrane. Cell Biochem Biophys 51: 89-95.
  • Popko J, Marciniak J, Zalewska A, Górska A, Zwierz K, Sierakowski S, Urban M (2006) Activity of N-acetyl-beta-hexosaminidase and its isoenzymes in serum and synovial fluid from patients with different artropathies. Clin Exp Rheumatol 24: 690-693.
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv61p85kz
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