Antimutagenic effects of aqueous fraction of Myristica fragrans (Houtt.) leaves on Salmonella typhimurium and Mus musculus

• Authors: Akeem Akinboro , Kamaruzaman Bin Mohamed , Mohd Asmawi , Taofeek Yekeen
• Languages of publication: EN

Abstracts

EN

Natural plant extracts offer a promising hope in the prevention/treatment of cancer arising from genetic mutations. This study evaluated in vitro and in vivo mutagenic and antimutagenic effects of aqueous fraction of Myristica fragrans (AFMF) leaves on TA100 strain of Salmonella typhimurium and Mus musculus (Male Swiss albino mice), respectively. The antioxidant activity of AFMF against 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and flavonoid contents were determined, followed by its phytochemical elucidation using the Ultra Performance Liquid Chromatography technique (UPLC). The mutagenicity of AFMF at 4, 20, 50, 100, 200, 500, and 1000 µg/well was <2.0 in S. typhimurium and the induced micronucleated polychromatic and normochromatic erythrocytes at 500, 1000, 2000, and 4000 mg/kg were not significantly different from the negative control (p≥0.05). The mutagenic activity of benzo[a]pyrene and cyclophosphamide was significantly suppressed above 50.0% throughout the tested concentrations. Fifty percent of the free radicals from DPPH were scavenged by AFMF at 0.11 mg/ml. Total phenolic and flavonoid contents of AFMF were 51.0 mg GAE/g and 27 mg QE/g, respectively. Rutin was elucidated by the UPLC technique, and thereby suspected to be the phytochemical responsible for the observed antimutagenic activity. Thus far, AFMF seems to contain a promising chemotherapeutic agent for the prevention of genetic damage that is crucial for cancer development.

Keywords

EN

benzo[a]pyrene; chromosome; aberrations; cyclophosphamide; micronucleus; Myristica fragrans; Salmonella typhimurium

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 779-785

Dates

published

2014

received

2014-04-19

revised

2014-10-24

accepted

2014-11-07

(unknown)

2014-12-16

Contributors

author

Akeem Akinboro

• School of Biological Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia

author

Kamaruzaman Bin Mohamed

• School of Biological Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia

author

Mohd Asmawi

• School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Pula Pinang, Malaysia

author

Taofeek Yekeen

• Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P.M.B.4000, Oyo State, Nigeria

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