Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer

• Authors: Anna Biernasiuk , Izabela Korona-Głowniak , Agnieszka Grzegorczyk , Anna Malm
• Languages of publication: EN

Abstracts

EN

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

Keywords

EN

RAPD-PCR; Candida albicans; upper respiratory tract; genetic diversity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 727-729

Dates

published

2014

received

2013-11-29

revised

2014-06-12

accepted

2014-10-17

(unknown)

2014-11-06

Contributors

author

Anna Biernasiuk

• Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland

author

Izabela Korona-Głowniak

• Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland

author

Agnieszka Grzegorczyk

• Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland

author

Anna Malm

• Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland

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