Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease

• Authors: Rukhshan Khurshid , Mahjabeen Saleem , Gul-e-Raana , Muhammad Akhthar
• Languages of publication: EN

Abstracts

EN

The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.

Keywords

EN

phosphorylation sites; HER2/c-erbB-2; cell growth and disease

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 699-703

Dates

published

2014

received

2013-06-06

revised

2014-10-09

accepted

2014-10-30

(unknown)

2014-11-14

Contributors

author

Rukhshan Khurshid

• Department of Biochemistry, Fatima Jinnah Medical College, Lahore, Pakistan

author

Mahjabeen Saleem

• Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

author

Gul-e-Raana

• Department of Biochemistry, Gujranwala Medical College, Gujranwala, Pakistan

author

Muhammad Akhthar

• Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

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