Preferences help
enabled [disable] Abstract
Number of results
2014 | 61 | 3 | 593-596
Article title

Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules

Title variants
Languages of publication
Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
Physical description
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Alexander DJ (2007) An overview of the epidemiology of avian influenza. Vaccine 25: 5637-5644.
  • Capua I, Cattoli G (2013) Prevention and control of highly pathogenic avian influenza with particular reference to H5N1. Virus Res 178: 114-120.
  • Chen HY, Zhang HY, Li XS, Cui BA, Wang SJ, Geng JW, Li K (2011) Interleukin-18-mediated enhancement of the protective effect of an infectious laryngotracheitis virus glycoprotein B plasmid DNA vaccine in chickens. J Med Microbiol 60: 110-116.
  • Gao H, Li K, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X (2013a) DNA prime-protein boost vaccination enhances protective immunity against infectious bursal disease virus in chickens. Vet Microbiol 164: 9-17.
  • Gao R, Cao B, Hu Y, Feng Z, Wang D, Hu W, Chen J, Jie Z, Qiu H, Xu K, Xu X, Lu H, Zhu W, Gao Z, Xiang N, Shen Y, He Z, Gu Y, Zhang Z, Yang Y, Zhao X, Zhou L, Li X, Zou S, Zhang Y, Yang L, Guo J, Dong J, Li Q, Dong L, Zhu Y, Bai T, Wang S, Hao P, Yang W, Han J, Yu H, Li D, Gao GF, Wu G, Wang Y, Yuan Z, Shu Y (2013b) Human infection with a novel avian-origin influenza A (H7N9) virus. N Engl J Med 368: 1888-1897.
  • Graham-Rowe D (2011) Epidemiology: Racing against the flu. Nature 480: S2-S3.
  • Haygreen EA, Kaiser P, Burgess SC, Davison TF (2006) In ovo DNA immunisation followed by a recombinant fowlpox boost is fully protective to challenge with virulent IBDV. Vaccine 24: 4951-4961.
  • Jalilian B, Omar AR, Bejo MH, Alitheen NB, Rasoli M, Matsumoto S (2010) Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant. Genet Vaccines Ther 8: 4.
  • Jiang Y, Yu K, Zhang H, Zhang P, Li C, Tian G, Li Y, Wang X, Ge J, Bu Z, Chen H (2007) Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Antiviral Res 75: 234-241.
  • Li J, Huang Y, Liang X, Lu M, Li L, Yu L, Deng R (2003) Plasmid DNA encoding antigens of infectious bursal disease viruses induce protective immune responses in chickens: factors influencing efficacy. Virus Res 98: 63-74.
  • Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X (2013) Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal disease virus in chickens. Vaccine 31: 1799-1805.
  • Lim KL, Jazayeri SD, Yeap SK, Alitheen NB, Bejo MH, Ideris A, Omar AR (2012) Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses. BMC Vet Res 8: 132.
  • Liniger M, Summerfield A, Ruggli N (2012) MDA5 can be exploited as efficacious genetic adjuvant for DNA vaccination against lethal H5N1 influenza virus infection in chickens. PLoS One 7: e49952.
  • Mahmood MS, Hussain I, Siddique M, Akhtar M, Ali S (2007) DNA vaccination with VP2 gene of very virulent infectious bursal disease virus (vvIBDV) delivered by transgenic E. coli DH5alpha given orally confers protective immune responses in chickens. Vaccine 25: 7629-7635.
  • Shan S, Jiang Y, Bu Z, Ellis T, Zeng X, Edwards J, Tian G, Li Y, Ge J, Chen H, Fenwick S (2011) Strategies for improving the efficacy of a H6 subtype avian influenza DNA vaccine in chickens. J Virol Methods 173: 220-226.
  • Song H, Qiu B, Yan R, Xu L, Song X, Li X (2013) The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens. Res Vet Sci 94: 562-567.
  • Song H, Song X, Xu L, Yan R, Shah MA, Li X (2010) Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase. Vet Parasitol 173: 219-227.
  • Stachyra A, Góra-Sochacka A, Sawicka R, Florys K, Sączyńska V, Olszewska M, Pikuła A, Śmietanka K, Minta Z, Szewczyk B, Zagórski W, Sirko A (2014) Highly immunogenic prime-boost DNA vaccination protects chickens against challenge with homologous and heterologous H5N1 virus. Trials Vaccinol 3: 40-46.
  • Xu Q, Song X, Xu L, Yan R, Shah MA, Li X (2008) Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Vet Parasitol 156: 319-323.
  • Yang T, Wang HN, Wang X, Tang JN, Gao R, Li J, Guo ZC, Li YL (2009) Multivalent DNA vaccine enhanced protection efficacy against infectious bronchitis virus in chickens. J Vet Med Sci 71: 1585-1590.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.