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2014 | 61 | 1 | 47-53
Article title

The influence of L-phenylalanine, methyl jasmonate and sucrose concentration on the accumulation of phenolic acids in Exacum affine Balf. f. ex Regel shoot culture

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Phenolic acids are an important group of plant secondary metabolites with various, valuable therapeutic properties. Apart from plants growing in the open air, tissue cultures can be an alternative source of the secondary metabolites. The yield of their accumulation in in vitro cultures can be increased by different methods, including culture medium supplementation with precursors, elicitors and changing the standard amounts of the medium components. The purpose of this study was to investigate the influence of the precursor (L-phenylalanine), the elicitor (methyl jasmonate) and a higher sucrose concentration on the phenolic acids accumulation in the agitated shoot cultures of Exacum affine Balf. f. ex Regel (Gentianaceae). Qualitative and quantitative analyses of the phenolic acids in methanolic extracts from the biomass were conducted by applying the HPLC method. Fourteen phenolic acids and cinnamic acid were found in all samples. The total content of free phenolic acids increased from approximately 0.242% to 0.635% (2.6-fold) and the total content of the whole phenolic acids (free and bound) - from 0.712% to 1.160% (1.6-fold). The studies show that the best variant for the accumulation of most of the identified phenolic acids contained 6% of sucrose (double the standard amount), L-phenylalanine 1.6 gL-1 of medium and methyl jasmonate 100 μM. The analysis of the results in the experiment presented here showed that it is possible to increase the accumulation of the phenolic acids in Exacum affine shoot cultures - by adding the precursor (L-phenylalanine), the elicitor (methyl jasmonate) and by increasing the sucrose concentration.
Physical description
  • Chair and Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Chair and Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Institute of Applied Informatics, Cracow University of Technology, Kraków, Poland
  • Abd El-Mawla AMA, Schmidt W, Beerhues L (2001) Cinnamic acid is a precursor of benzoic acids in cell cultures of Hypericum androsaemum L. but not in cell cultures of Centaurium erythraea RAFN. Planta 212: 288-293.
  • Anderson TW, Darling DA (1954) A Test of Goodness of Fit. J Am Stat Assoc 49: 765-769.
  • Arora R (2010) Medicinal Plant Biotechnology. CABI, Wallingford, Oxfordshire, UK.
  • Bartlett MS (1937) Properties of sufficiency and statistical tests. J R Stat Soc 160: 268-282.
  • Beerhues L, Berger U (1995) Differential accumulation of xanthones in methyl-jasmonate and yeast-extract-treated cell cultures of Centaurium erythraea and Centaurium littorale. Planta 197: 608-612.
  • Box GEP, Cox DR (1964) An Analysis of Transformations. J Roy Stat Soc B 26: 211-252.
  • Cousins MM, Adelberg J, Chen F, Rieck J (2010) Secondary Metabolism-Inducing Treatments During in vitro Development of Turmeric (Curcuma longa L.) Rhizomes. J Herbs Spices Med Plants 15: 303-317.
  • Daniel M, Sabnis SD (1978) Chemical Systematics of Family Gentianaceae. Curr Sci 47: 109-111.
  • Ellnain-Wojtaszek M, Zgorka G (1999) High-performance liquid chromatography and thin-layer chromatography of phenolic acids from Ginkgo biloba L. - leaves collected within vegetative period. J Liq Chromatogr R T 22: 1457-1471.
  • Everitt BS, Landau S, Leese M, Stahl D (2011) Cluster Analysis. John Wiley & Sons, Ltd.
  • Fisher RA (1935) The Design of Experiments. Oliver and Boyd Press, Edinburgh.
  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158.
  • Giberti S, Bertea CM, Narayana R, Maffei ME, Forlani G (2012) Two phenylalanine ammonia lyase isoforms are involved in the elicitor-induced response of rice to the fungal pathogen Magnaporthe oryzae. J Plant Physiol 169: 249-254.
  • Grzegorczyk I, Wysokińska H (2009) The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L. Herba Pol 55: 238-243.
  • Hegnauer R (1966) Chemotaxonomie der Pflanzen, vol. 17. Birkhauser, Basel.
  • Jaremicz Z, Łuczkiewicz M, Kokotkiewicz A, Królicka A, Sowiński P (2014) Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors. Biotechnol Lett, DOI 10.1007/s10529-013-1426-9.
  • Jeeshna MV, Paulsamy S (2011) Evaluation of certain flavonoids of medicinal importance in the wild and micropropagated plants of the endangered medicinal species, Exacum bicolor Roxb. J Appl Pharm Sci 01: 99-102.
  • Jolliffe IT (2010) Principal component analysis. Second edition. Springer-Verlag New York Inc., New York.
  • Keil M, Härtle B, Guillaume A, Psiorz M (2000) Production of amarogentin in root cultures of Swertia chirata. Planta Med 66: 452-457.
  • Kempthorne O, Hinkelmann K (2007) Design and analysis of experiments. Vol. 1. Introduction to experimental design. John Wiley & Sons, Hoboken, NJ, USA.
  • Khadem S, Marles RJ (2010) Monocyclic phenolic acids; hydroxy- and polyhydroxybenzoic acids: occurrence and recent bioactivity studies. Molecules 15: 7985-8005.
  • Kikowska M, Budzianowski J, Krawczyk A, Thiem B (2012) Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L. Acta Physiol Plant 34: 2425-2433.
  • Krolicka A, Szpitter A, Gilgenast E, Romanik G, Kaminski A, Lojkowska E (2008) Stimulation of antibacterial naphthoquinones and flavonoids accumulation in carnivorous plants grown in vitro by addition of elicitors. Enzyme Microb Technol 42: 216-221.
  • Kruskal WH, Wallis WA (1952) Use of Ranks in One-Criterion Variance Analysis. J Am Stat Assoc 47: 583-621.
  • Kumar V, Chauhan RS, Sood H (2013) In vitro production and efficient quantification of major phytopharmaceuticals in an endangered medicinal herb, Swertia chirata. Int J Biotech Bioeng Res 4: 495-506.
  • Levene H (1960) Robust tests for equality of variances. In In Contributions to Probability and Statistics: Essays in Honor of Harold Hotelling. Olkin I, Ghurye SG, Hoeffding W, Madow WG and Mann HB, eds, pp 278-292. Stanford University Press, Stanford.
  • Mothana RAA, Grunert R, Lindequist U, Bednarski PJ (2007) Study of the anticancer potential of Yemeni plants used in folk medicine. Pharmazie 62: 305-307.
  • Mothana RAA, Lindequist U (2005) Antimicrobial activity of some medicinal plants of the island Soqotra. J Ethnopharmacol 96: 177-181.
  • Mothana RAA, Mentel R, Reiss C, Lindequist U (2006) Phytochemical screening and antiviral activity of some medicinal plants from the Island Soqotra. Phytother Res 20: 298-302.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473-497.
  • Namdeo AG (2007) Plant cell elicitation for production of secondary metabolites. Pharmacogn Rev 1: 69-79.
  • Qu JG, Zhang W, Yu XJ (2011) A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera. Plant Cell Tiss Org 107: 261-269.
  • Riedel H, Akumo DN, Saw NMMT, Kutuk O, Neubauer P, Smeta I (2012) Elicitation and precursor feeding influence phenolic acids composition in Vitis vinifera suspension culture. Afr J Biotechnol 11: 3000-3008.
  • Riseman A (2006) Exacum. In Flower Breeding and Genetics. Anderson NO, eds, pp 601-622. Springer Netherlands.
  • Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52: 591.
  • Skrzypczak-Pietraszek E, Pietraszek J (2012) Chemical profile and seasonal variation of phenolic acid content in bastard balm (Melittis melissophyllum L., Lamiaceae). J Pharm Biomed Anal 66: 154-161.
  • Skrzypczak-Pietraszek E, Szewczyk A, Piekoszewska A, Ekiert H (2005) Biotransformation of hydroquinone to arbutin in plant in vitro cultures preliminary results. Acta Physiol Plant 27: 79-87.
  • Thiem B, Krawczyk A (2010) Enhanced isoflavones accumulation in methyl jasmonate-treated in vitro cultures of kudzu (Puerara lobata Ohwi). Herba Pol 56: 48-56.
  • Weremczuk-Jeżyna I, Grzegorczyk-Karolak I, Frydrych B, Królicka A, Wysokińska H (2013) Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential. Acta Physiol Plant 35: 2095-2103.
  • Yuan YM, Wohlhauser S, Moller M, Klackenberg J, Callmander MW, Kupfer P (2005) Phylogeny and biogeography of Exacum (Gentianaceae): A disjunctive distribution in the Indian Ocean Basin resulted from long distance dispersal and extensive radiation. Syst Biol 54: 21-34.
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