The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

• Authors: Iwona Dąbrowska , Bożena Dworecka-Kaszak , Anna Brillowska-Dąbrowska
• Languages of publication: EN

Abstracts

EN

Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.

Keywords

EN

dermatophytosis; animals; pan-dermatophytes; PCR

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 2
• Pages: 375-378

Dates

published

2014

received

2014-02-12

revised

2014-04-02

accepted

2014-04-28

(unknown)

2014-06-18

Contributors

author

Iwona Dąbrowska

• Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warszawa, Poland

author

Bożena Dworecka-Kaszak

• Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warszawa, Poland

author

Anna Brillowska-Dąbrowska

• Gdansk University of Technology, Department of Microbiology, Gdańsk, Poland

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