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2014 | 61 | 2 | 375-378
Article title

The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

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EN
Abstracts
EN
Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.
Keywords
Publisher

Year
Volume
61
Issue
2
Pages
375-378
Physical description
Dates
published
2014
received
2014-02-12
revised
2014-04-02
accepted
2014-04-28
(unknown)
2014-06-18
Contributors
  • Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warszawa, Poland
  • Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warszawa, Poland
  • Gdansk University of Technology, Department of Microbiology, Gdańsk, Poland
References
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv61p375kz
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