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2014 | 61 | 2 | 305-310
Article title

The effect of thyme and tea tree oils on morphology and metabolism of Candida albicans

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Abstracts
EN
Members of Candida species cause significant problems in medicine and in many industrial branches also. In order to prevent from Candida sp. development, essential oils are more and more frequently applied as natural, non-toxic, non-pollutive and biodegradable agents with a broad spectrum of antimicrobial activity. The aim of the research was to determine changes in morphology and metabolic properties of Candida albicans in the presence of thyme and tea tree oils. Changes of enzymatic activity of isolates were observed in the presence of both tested essential oils, and they were primarily associated with loss or decrease of activity of all enzymes detected for control. Furthermore, only for 3 out of 11 isolates additional activity of N-acetyl-β-glucosaminidase, α-mannosidase, α-fucosidase and trypsin was detected. Vivid changes in biochemical profiles were found after treatment with tea tree oil and they were related to loss of ability to assimilate d-xylose, d-sorbitol and d-trehalose. The main differences in morphology of isolates compared to the control strain concerned formation of pseudohyphae structures. Both examined essential oils caused changes in cell and colony morphology, as well as in the metabolism of Candida albicans. However, the extent of differences depends on the type and concentration of an essential oil. The most important finding is the broad spectrum of changes in yeast enzymatic profiles induced by thyme and tea tree oils. It can be supposed that these changes, together with loss of ability to assimilate saccharides could significantly impact Candida albicans pathogenicity.
Year
Volume
61
Issue
2
Pages
305-310
Physical description
Dates
published
2014
received
2013-10-30
revised
2014-01-22
accepted
2014-04-03
(unknown)
2014-06-13
References
  • Abaci O (2011) Investigation of extracellular phospholipase and proteinase activities of Candida species isolated from individuals denture wearers and genotypic distribution of Candida albicans strains. Curr Microbiol 62: 1308-1314.
  • Adorian B, Buchbauer G (2010) Biological properties of essential oils: an updated review. Flavour Fragr J 25: 407-426.
  • Batish DR, Singh HP, Kohli RK, Kaur S (2008) Eucalyptus essential oil as a natural pesticide. For Ecol Manage 256: 2166-2174.
  • Batura-Gabryel H, Młynarczyk W (2000) Hydrolitic activity of Candida strains and oral candidiasis in lung cancer and COPD patients. Mikol Lek 7: 77-82 (in Polish).
  • Budzyńska A, Sadowska B, Lipowczan G, Maciąg A, Kalemba D, Różalska B (2013) Activity of selected essential oils against Candida spp. strains. Evaluation of new aspects of their specific pharmacological properties, with special reference to Lemon balm. Adv Microbiol 3: 317-325.
  • Budzyńska A, Więckowska-Szakiel M, Sadowska B, Kalemba D, Różalska B (2011) Antibiofilm activity of selected plant essential oils and their major components. Pol J Microbiol 60: 35-41.
  • Burt S (2004) Essential oils: their antibacterial properties and potential applications in food - a review. Int J Food Microbiol 94: 223-253.
  • Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, Wyllie SG (2000) The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol 88: 170-175.
  • Custódio JBA, Ribeiro MV, Silva FSG, Machado M, Sousa MC (2011) The essential oils component p-cymene induces proton leak through Fo-ATP synthase and uncoupling of mitochondrial respiration. J Exp Pharmacol 3: 69-76.
  • Donaldson JR, Warner SL, Cates RG, Young DG (2005) Assessment of antimicrobial activity of fourteen essential oils when using dilution and diffusion methods. Pharm Biol 43: 687-695.
  • Dorman HJ, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88: 308-316.
  • Ernst JF (2000) Transcription factors in Candida albicans - environmental control of morphogenesis. Microbiology 146: 1763-1774.
  • Gasch AP (2007) Comparative genomics of the environmental stress response in ascomycete fungi. Yeast 24: 961-976.
  • Granek JA, Magwene PM (2010) Environmental and genetic determinants of colony morphology in yeast. PLoS Genet 6: e1000823.
  • Hagerty JA, Ortiz J, Reich D, Manzarbeitia C (2003) Fungal infections in solid organ transplant patients. Surg Infect (Larchmt) 4: 263-271.
  • Kalemba D, Kunicka A (2003) Antibacterial and antifungal properties of essential oils. Curr Med Chem 10: 813-829.
  • Kojic EM, Darouiche RO (2004) Candida infections of medical devices. Clin Microbiol Rev 17: 255-267.
  • Kotzekidou P, Giannakidis P, Boulamatisis A (2008) Antimicrobial activity of some plant extracts and essential oils against foodborne pathogens in vitro and on the fate of inoculated pathogens in chocolate. Food Sci Technol 41: 119-127.
  • Kourkoumpetis TK, Velmahos GC, Ziakas PD, Tampakakis E, Manolakaki D, Coleman JJ, Mylonakis E (2010) The effect of cumulative length of hospital stay on the antifungal resistance of Candida strains isolated from critically ill surgical patients. Mycopathologia 171: 85-91.
  • Kwaśniewska J, Loga G, Woźniacka A, Dziankowska-Bartkowiak B, Sysa-Jędrzejewska A (2001) Fungi in patients with bollous and conective tissue diseases-activity of selected hydrolases in Candida albicans strains. Mikol Lek 8: 85-90 (in Polish).
  • Lass-Flörl C (2009) The changing face of epidemiology of invasive fungal disease in Europe. Mycoses 52: 197-205.
  • Louie A, Dixon DM, El-Maghrabi EA, Burnett JW, Baltch AL, Smith RP (1994) Relationship between Candida albicans epidermolytic proteinase activity and virulence in mice. J Med Vet Mycol 32: 59-64.
  • Low WL, Martin C, Hill DJ, Kenward MA (2013) Antimicrobial efficacy of liposome-encapsulated silver ions and tea tree oil against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Lett Appl Microbiol 57: 33-39.
  • Madhani HD (2000) Interplay of intrinsic and extrinsic signals in yeast differentiation. Proc Natl Acad Sci USA 97: 13461-13463.
  • Manolakaki D, Velmahos G, Kourkoumpetis T, Chang Y, Alam HB, De Moya MM, Mylonakis E (2010) Candida infection and colonization among trauma patients. Virulence 1: 367-375.
  • Merson-Davies LA, Hopwood V, Robert T, Marot-Leblond A, Senet JM, Odds FC (1991) Reaction of Candida albicans cells of different morphology index with monoclonal antibodies specific for hyphal form. J Med Microbiol 35: 321-324.
  • Merson-Davies LA, Odds FC (1989) A morphology index for characterization of cell shape in Candida albicans. J Gen Microbiol 135: 3143-3152.
  • Nawrot U, Pajączkowska M, Fleischer M, Przondo-Mordarska H, Samet A, Piasecka-Pazik D, Komarnicka J, Sulik-Tyszka B, Swoboda-Kopeć E, Cieślik J, Mikucka A, Gospodarek E, Ozorowski T, Mól A, Tryniszewska E, Kłosowska W, Krawczyk M, Golec K, Szymaniak L, Giedrys-Kalemba S, Bilska I, Prawda-Zołotar J, Juszczyk-Grudzińska M, Wróblewska M, Burdynowski K (2013) Candidaemia in polish hospitals - a multicentre survey. Mycoses 56: 576-581.
  • Ninomiya K, Maruyama N, Inoue S, Ishibashi H, Takizawa T, Oshima H, Abe S (2012) The essential oil of Melaleuca alternifolia (tea tree oil) and its main component, terpinen-4-ol protect mice from experimental oral candidiasis. Biol Pharm Bull 35: 861-865.
  • Nowicki R, Korting HC (1995) Differences in the hydrolitc activity of dermatophytes. Mikol Lek 2: 209-213 (in Polish).
  • Reynolds TB (2006) The Opi1p transcription factor effects of FLO11, mat formation, and invasive growth in Saccharomyces cerevisiae. Eukaryot Cell 5: 1266-1275.
  • Rustchenko-Bulgac EP (1991) Variations of Candida albicans electrophoretic karyotypes. J Bacteriol 173: 6586-6596.
  • Samaranayake LP, Fidel PL, Naglik JR, Sweet SP, Teanpaisan R, Coogan MM, Blignaut E, Wanzala P (2002) Fungal infections associated with HIV infection. Oral Dis 8: 151-160.
  • Schaller M, Borelli C, Korting HC, Hube B (2005) Hydrolytic enzymes as virulence factors of Candida albicans. Mycoses 48: 365-377.
  • Sudbery PE (2011) Growth of Candida albicans hyphae. Nat Rev Microbiol 9: 737-748.
  • Tullio V, Mandras N, Allizond V, Nostro A, Roana J, Merlino C, Banche G, Scalas D, Cuffini AM (2012) Positive interaction of thyme (red) essential oil with human polymorphonuclear granulocytes in eradicating intracellular Candida albicans. Planta Med 78: 1633-1635.
  • Uribe S, Ramirez T, Pena A (1985) Effects of β-pinene on yeast membrane functions. J Bacteriol 161: 1195-1200.
  • Voordeckers K, De Maeyer D, van der Zande E, Vinces MD, Meert W, Cloots L, Ryan O, Marchal K, Verstrepen KJ (2012) Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology. Mol Microbiol 86: 225-239.
  • Vopálenská I, Hůlková M, Janderová B, Palková Z (2005) The morphology of Saccharomyces cerevisiae colonies is affected by cell adhesion and the budding pattern. Res Microbiol 156: 921-931.
  • Wróblewska J, Ciok-Pater E, Gospodarek E, Sękowska A (2011) Effect of different carbon sources on lipolytic activity of Candida spp. Mikol Lek 18: 71-73 (in Polish).
  • Zhu W, Filler SG (2010) Interactions of Candida albicans with epithelial cells. Cell Microbiol 12: 273-82.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv61p305kz
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