PL EN


Preferences help
enabled [disable] Abstract
Number of results
2014 | 61 | 2 | 275-280
Article title

N-acetyl-beta-glucosaminidase urine activity as a marker of early proximal tubule damage and a predictor of the long-term function of the transplanted kidneys

Content
Title variants
Languages of publication
EN
Abstracts
EN
Introduction: Ischaemia-reperfusion injury (IRI) is a factor leading to the damages of the transplanted kidney, what affects mainly the proximal tubules. Early monitoring of tubule damage can be an efficient tool to predict the allograft dysfunction. Present in proximal tubules, N-acetyl-beta-glucosaminidase (NAG) is a lysosomal enzyme whose excretion rises as a result of IRI or acute rejection. The aim of this study was to monitor the NAG urine activity to evaluate the early proximal tubule damage, and to try to predict the long-term function of the transplanted kidney. Material and methods: The study enrolled 87 Caucasian renal transplant recipients (61.7% males, 38.3% females, mean age 45.56±14.34 years). Urine samples were collected for NAG and creatinine analysis on the 1st day after transplantation, and then in the 3rd and 12th month. Protocol biopsies were performed in the 3rd and 12th month. Results: A significant positive correlation between NAG urine activity in the 3rd month after transplantation and creatinine concentration on the 14th (p=0.004) and 30th day (p=0.05), in the 3rd month (p=0.009) and after the 1st (p=0.005) and 2nd year (p=0.003) was observed. A statistically significantly higher urinary NAG activity in samples collected in the first 3 days and in the 3rd month after transplantation among patients with DGF (p=0.006 and p=0.03 respectively) was found. There was a significant positive correlation between NAG urine activity in the 3rd month and the grade of tubular atrophy in specimens collected in the 3rd (p=0.03) and 12th (p=0.04) month. Conclusions: Monitoring of NAG urine activity is useful in the evaluation of early proximal tubule damage and predicting the long-term function of the transplanted kidneys.
Keywords
EN
Publisher

Year
Volume
61
Issue
2
Pages
275-280
Physical description
Dates
published
2014
received
2013-06-27
revised
2014-02-27
accepted
2014-05-06
(unknown)
2014-06-11
Contributors
  • Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Poland
  • Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Poland
author
  • Department of Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
  • Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Poland
  • Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
  • Department of Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
  • Clinical Department of Surgery and Transplantology, Pomeranian Medical University in Szczecin, Poland
author
  • Department of Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
  • Department of Pharmacology, Pomeranian Medical University, Szczecin, Poland
  • Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Poland
References
  • Bazzi C, Petrini C, Rizza V, Arrigo G, Napodano P, Paparella M, D'Amico G (2002) Urinary N-acetyl-beta-glucosaminidase excretion is a marker of tubular cell dysfunction and a predictor of outcome in primary glomerulonephritis. Nephrol Dial Transplant 17: 1890-1896.
  • Burne-Taney MJ, Yokota N, Rabb H (2005) Persistent renal and extrarenal immune changes after severe, ischemic injury. Kidney Int 67: 1002-1009.
  • Cecka JM (2002) The UNOS Renal Transplant Registry. Clin Transpl 1-20.
  • Cosio FG, Grande JP, Larson TS, Gloor JM, Velosa JA, Textor SC, Griffin MD, Stegall MD et al. (2005) Kidney allograft fibrosis and atrophy early after living donor transplantation. Am J Transplant 5: 1130-1136.
  • Doi K, Katagiri D, Negishi K, Hasegawa S, Hamasaki Y, Fujita T, Matsubara T, Ishii T, Yahagi N, Sugaya T, Noiri E (2012) Mild elevation of urinary biomarkers in prerenal acute kidney injury. Kidney Int 82: 1114-11120
  • Domański L, Kłoda K, Pawlik A, Wiśniewska M, Kwiatkowska E, Kurzawski M, Safranow K, Ciechanowski K (2013) Correlation between ICAM1 and VCAM1 gene polymorphisms and histopathological changes in kidney allograft biopsies. Arch Med Sci 9: 276-282.
  • Herget-Rosenthal S, Poppen D, Husing J, Marggraf G, Pietruck F, Jakob HG, Philipp T, Kribben A (2003) Prognostic value of tubular proteinurua and enzymuria in nonoliguric acute tubular necrosis. Clin Chem 50: 552-558.
  • Holdt-Lehmann B, Lehmann A, Korten G, Nagel H, Nizze H, Schuff-Werner P (2000) Diagnostic value of urinary alanine aminopeptidase and N-acetyl-beta-D-glucosaminidase in comparison to alpha 1-microglobulin as a marker in evaluating tubular dysfunction in glomerulonephritis patients. Clin Chim Acta 297: 93-102.
  • Hultberg B, Ravnskov U (1981) The excretion of N-acetyl-beta-glucosaminidase in gloerulonephritis. Clin Nephrol 15: 33-38.
  • Kłoda K, Domański L, Pawlik A, Kurzawski M, Safranow K, Ciechanowski K ( 2010) Effect of the ICAM1 and VCAM1 gene polymorphisms on delayed graft function and acute kidney allograft rejection. Ann Transplant 15: 15-20.
  • Kłoda K, Domański L, Pawlik A., Safranow K, Ciechanowski K (2013) The impact of ICAM1 and VCAM1 gene polymorphisms on long-term renal transplant function and recipient outcomes. Ann Transplant 18: 231-237.
  • Kuźniar J, Marchewka Z, Krasnowski R, Boratyńska M, Długosz A, Klinger M (2006) Enzymuria and low molecular weight protein excretion as the differentiating marker of complications in the early post kidney transplantation period. Int Urol Nephrol 38: 753-758.
  • Liu Y (2004) Epithelial to mesenchymal transition in renal fibrogenesis: pathologic significance, molecular mechanism, and therapeutic intervention. J Am Soc Nephrol 15: 1-12.
  • Maruhn D (1976) Rapid colorimetric assay of beta-galactosidase and N-acetyl-beta-glucosaminidase in human urine. Clin Chim Acta 73: 453-461.
  • Matteucci E, Carmellini M, Bertoni C, Boldrini E, Mosca F, Giampietro O (1998) Urinary excretion rates of multiple renal indicators after kidney transplantation: clinical significance for early graft outcome. Ren Fail 20: 325-30.
  • Meier-Kriesche HU, Schold JD, Srinivas TR, Kaplan B (2004) Lack of improvement in renal allograft survival despite a marked decrease in acute rejection rates over the most recent era. Am J Transplant 4: 378-383.
  • Nankivell BJ, Borrows RJ, Fung CL, O'Connell PJ, Allen RD, Chapman JR (2003) The natural history of chronic allograft nephropathy. N Engl J Med 349: 2326-2333.
  • Nankivell BJ, Borrows RJ, Fung CL, O'Connell PJ, Chapman JR, Allen RD (2004) Delta analysis of posttransplantation tubulointerstitial damage. Transplantation 78: 434-441.
  • Nauta FL, Bakker SJ, van Oeveren W, Navis G, van der Heide JJ, van Goor H, de Jong PE, Gansevoort RT (2011) Albuminuria, proteinuria, and novel urine biomarkers as predictors of long-term allograft outcomes in kidney transplant recipients. Am J Kidney Dis 57: 733-743.
  • Perez-Blanco FJ, Garbin-Fuentes I, Perez-Chica G, Moreno-Terribas G, Rodríguez-Cuartero A et al. (1997) Urinary activity of N-acetyl-beta-glucosaminidase and progression of retinopathy in non-insulin-dependent diabetes mellitus. Clin Nephrol 48: 388-389.
  • Pergande M, Jing K, Precht S, Fels LM, Herbort C, Stolte H et al. (1994) Changed excretion of urinary proteins and enzymes by chronic exposure to lead. Nephrol Dial Transplant 9: 613-618.
  • Peto K, Olah VA, Brath E, Nemeth N, Gulyas A, Szilasi M, Sarvari M, Furka I, Miko I (2005) Determination of urinary NAG to detect renal ischemia-reperfusion injury and the protective effect of allopurynol. Magy Seb 58: 134-137.
  • Price RG (1992) The role of NAG (N-acetyl-β-D-glucosaminidase) in the diagnosis of kidney disease including the monitoring of nephrotoxicity. Clin Nephrol 38 Suppl 1: S14-S19.
  • Sanchez-Bernal C, Viltos M, Cabezas JA, Price RG et al. (1991) Variation in the isoenzymes of N-acetyl-β-D-glucosaminidase and protein exctretion in aminoglycoside nephrotoxicity in the rat. Cell Bichem Funct 9: 209.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv61p275kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.